FastQCFastQC Report
Thu 2 Feb 2017
SRR4062974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027101
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27900.27163832962873175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18270.17787929327300822No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11320.11021311438699796No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11310.1101157529785289No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11260.10962894593618348No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11170.10875269325996177No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11070.10777907917527098No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11030.10738963354139466No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10910.1062212966397657No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10600.10320309297722424No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10470.1019373946671262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10390.10115850339937357No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10370.10096378058243541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGC308.417595E-426.66593732
GATACTA450.00884856417.77815611
TAGACTG904.37356E-717.7746945
TCTAGCG2150.017.11497728
TTCTATA908.545618E-616.0065772
CTACGCA600.00244370215.9980044
CATCGTT2700.015.40623628
CGGTCCA2600.015.38494410
GAACTCG859.4328294E-515.05841231
AAACGCT2450.015.01999729
CTAGCGG2450.015.01999729
CGATTTG2150.014.88258828
ATCGTTT2800.014.85673629
CGCCGGT2600.014.7681077
CGAGCCG2750.014.54576415
AAGACGG3300.014.54293255
CGCAAGA3200.014.50595952
GCGCAAG3000.014.41013051
TAGCGGC2800.014.28532330
GTCTTAC901.5247932E-414.2322271
Produced by FastQC (version 0.11.5)