FastQCFastQC Report
Thu 2 Feb 2017
SRR4062974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027101
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33970.33073670456946297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24900.2424299070880079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14090.13718222453293297No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12980.12637510819286515No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12650.12316218171338554No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11990.1167363287544263No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11630.11323131804953943No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11510.11206298114791048No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.11186825833097232No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10990.10700018790751833No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10910.1062212966397657No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10800.10515032114660583No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10620.1033978157941624No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10410.10135322621631174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2600.020.3065829
TCTAGCG2750.019.19894828
TAGCGGC2700.018.96192630
GCTCGTT450.0088499617.7776712
ACTAATC550.001365012417.4535927
TTCGGAA3050.017.31052831
TAAACGC2700.017.18424428
TTTCGGA3150.016.76098830
GTTTTCG3250.016.73754528
TCCAATA2300.016.6955492
GTAAACG2800.016.57052227
CGCTTCG2750.016.29001632
CGCCGGT3550.016.2284137
TTAGAGT1601.8189894E-1116.0006834
TAAGACT1001.3134522E-616.0006834
GTCCTAG1304.798494E-915.9999031
TTTTCGG3300.015.99912429
AACGCTT2850.015.71843830
GTCCTAC1551.891749E-1015.4837771
AAACGCT3000.015.4658229
Produced by FastQC (version 0.11.5)