##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1027101
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20767869956314	32.0	32.0	32.0	32.0	32.0
2	31.309840025469743	32.0	32.0	32.0	32.0	32.0
3	31.38498356052618	32.0	32.0	32.0	32.0	32.0
4	31.481171764023205	32.0	32.0	32.0	32.0	32.0
5	31.420306279518762	32.0	32.0	32.0	32.0	32.0
6	34.9364921268697	36.0	36.0	36.0	36.0	36.0
7	34.96125113304339	36.0	36.0	36.0	36.0	36.0
8	34.90567432024699	36.0	36.0	36.0	36.0	36.0
9	34.99787362683904	36.0	36.0	36.0	36.0	36.0
10	34.85754662881255	36.0	36.0	36.0	32.0	36.0
11	35.03348940367111	36.0	36.0	36.0	36.0	36.0
12	34.9260374588283	36.0	36.0	36.0	32.0	36.0
13	34.980612422731554	36.0	36.0	36.0	36.0	36.0
14	34.923677418287006	36.0	36.0	36.0	32.0	36.0
15	34.89951426393315	36.0	36.0	36.0	32.0	36.0
16	34.90586709583575	36.0	36.0	36.0	32.0	36.0
17	34.87683781828662	36.0	36.0	36.0	32.0	36.0
18	34.87535403042155	36.0	36.0	36.0	32.0	36.0
19	34.865148607585816	36.0	36.0	36.0	32.0	36.0
20	34.853510024817425	36.0	36.0	36.0	32.0	36.0
21	34.846945918658434	36.0	36.0	36.0	32.0	36.0
22	34.82448561533871	36.0	36.0	36.0	32.0	36.0
23	34.774697911889874	36.0	36.0	36.0	32.0	36.0
24	34.74370874918825	36.0	36.0	36.0	32.0	36.0
25	34.72471645923819	36.0	36.0	36.0	32.0	36.0
26	34.672189979369115	36.0	36.0	36.0	32.0	36.0
27	34.64585079753598	36.0	36.0	36.0	32.0	36.0
28	34.626093246915346	36.0	36.0	36.0	32.0	36.0
29	34.58933152630559	36.0	36.0	36.0	32.0	36.0
30	34.56579051135186	36.0	36.0	36.0	32.0	36.0
31	34.55366025347069	36.0	36.0	36.0	32.0	36.0
32	34.53397767113458	36.0	36.0	36.0	32.0	36.0
33	34.49087772283349	36.0	36.0	36.0	32.0	36.0
34	34.463832670788946	36.0	36.0	36.0	32.0	36.0
35	34.4446612358473	36.0	36.0	36.0	32.0	36.0
36	34.41091966612826	36.0	36.0	36.0	32.0	36.0
37	34.40332255542542	36.0	36.0	36.0	32.0	36.0
38	33.98083538035694	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	33.0
22	76.0
23	230.0
24	712.0
25	1634.0
26	3618.0
27	6990.0
28	12089.0
29	18788.0
30	28426.0
31	40977.0
32	58289.0
33	92418.0
34	226016.0
35	536792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02078079388046	18.424137736293652	12.09467130669149	26.46041016313439
2	15.670610777323748	20.91673554986316	36.84155696469967	26.571096708113416
3	17.798931166457827	24.85325201708498	29.039889942663866	28.307926873793328
4	12.004381267646773	16.44192386330445	36.34933307370265	35.20436179534612
5	14.017914516600136	37.06124038555155	33.46694576964268	15.453899328205628
6	33.667445883989764	36.15026399597313	16.811621859236546	13.370668260800567
7	29.583155648243885	30.90737203265901	21.201287511026212	18.308184808070894
8	27.839933792566267	32.923593700557404	19.709368838692402	19.52710366818392
9	27.46884581269613	14.035960582979332	18.63204906388957	39.863144540434966
10	15.560010437275093	26.817955228763264	31.81032199772561	25.811712336236038
11	37.24977775873703	21.241298998964982	22.448850430899764	19.060072811398225
12	24.536970776802537	24.01267652943496	28.875323120061925	22.575029573700583
13	29.499075065719015	19.459935741407847	25.361113815597314	25.679875377275824
14	23.59660831797457	19.832908350785367	25.382411272114425	31.188072059125638
15	25.18953832193718	27.019835439747407	22.6279596651157	25.162666573199715
16	25.60517417469168	25.875157360376438	23.879443209577246	24.640225255354633
17	23.914590678034582	25.81469592571714	25.536826465946387	24.73388693030189
18	24.87106915483482	24.653076961272554	26.651614592917344	23.824239290975278
19	25.39798909746948	25.074651859943664	25.88674336798426	23.640615674602593
20	25.58034701553207	24.16889867695582	25.51326500509687	24.737489302415245
21	26.762632655048197	24.378249440171356	24.6880537435498	24.17106416123065
22	25.379003622818008	24.370242069669874	25.403538697752214	24.8472156097599
23	24.046636160062313	24.086749099406095	25.90624087235907	25.960373868172525
24	24.519691831669913	25.22215439377432	25.522124893267556	24.736028881288206
25	24.74561848468356	24.470279885385928	25.68934445462414	25.09475717530637
26	24.440634367993024	25.22332273067595	26.033077564913288	24.302965336417742
27	25.165319832538213	24.804498101450687	25.25440560802259	24.77577645798851
28	24.307930768249665	24.586871203513578	26.167144224375207	24.93805380386155
29	24.274341082327833	24.927636133155357	26.028306855898297	24.76971592861851
30	24.42456973559562	24.983716304433546	26.221861335934832	24.369852624036
31	24.859969954269346	24.856075497930583	25.033662706978184	25.250291840821887
32	24.438102971372825	25.064234189237478	25.053329711488935	25.44433312790076
33	24.22936011161512	24.53731424660282	25.763775909087812	25.46954973269425
34	24.849454922154685	24.65979489845692	25.977386839268974	24.513363340119422
35	25.712271724007667	24.587747456189803	25.679753013578992	24.020227806223534
36	24.58005590492074	25.262072571246648	25.27648205970007	24.881389464132546
37	25.33412001351376	25.113791146148234	25.295078088717666	24.257010751620335
38	24.56839645604128	24.812189660208354	25.662447668191994	24.956966215558367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	272.0
1	221.5
2	171.0
3	171.0
4	549.5
5	928.0
6	928.0
7	1061.0
8	1194.0
9	1145.5
10	1097.0
11	1097.0
12	1414.5
13	1732.0
14	2267.0
15	2802.0
16	2802.0
17	4144.0
18	5486.0
19	5486.0
20	6381.0
21	7276.0
22	7798.5
23	8321.0
24	8321.0
25	9597.5
26	10874.0
27	10874.0
28	13924.5
29	16975.0
30	20449.0
31	23923.0
32	23923.0
33	30125.5
34	36328.0
35	36328.0
36	40272.0
37	44216.0
38	50229.0
39	56242.0
40	56242.0
41	60324.5
42	64407.0
43	72981.5
44	81556.0
45	81556.0
46	84974.0
47	88392.0
48	88392.0
49	92723.5
50	97055.0
51	97166.0
52	97277.0
53	97277.0
54	92367.5
55	87458.0
56	87458.0
57	83907.5
58	80357.0
59	71545.5
60	62734.0
61	62734.0
62	59414.0
63	56094.0
64	45920.0
65	35746.0
66	35746.0
67	29601.0
68	23456.0
69	23456.0
70	18858.0
71	14260.0
72	11156.0
73	8052.0
74	8052.0
75	5917.0
76	3782.0
77	3782.0
78	3772.5
79	3763.0
80	2862.5
81	1962.0
82	1962.0
83	1957.5
84	1953.0
85	1953.0
86	1219.5
87	486.0
88	424.5
89	363.0
90	363.0
91	214.5
92	66.0
93	44.0
94	22.0
95	22.0
96	16.0
97	10.0
98	10.0
99	11.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00924933380456255
2	0.0
3	0.0
4	9.736140846907947E-5
5	9.736140846907947E-5
6	1.9472281693815895E-4
7	2.920842254072384E-4
8	0.0025313966201960664
9	0.003115565071010543
10	0.0012656983100980332
11	0.006231130142021086
12	5.841684508144768E-4
13	9.736140846907947E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	9.736140846907947E-5
22	0.0
23	9.736140846907947E-5
24	0.0
25	1.9472281693815895E-4
26	0.0
27	9.736140846907947E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.736140846907947E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1027101.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.46247484611725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79358982430381	48.40318162523111
2	11.004721469297435	12.867265041746556
3	2.72504395828914	4.779384415981279
4	1.1032140881066295	2.5798650350326406
5	0.5944064716578417	1.737523669883294
6	0.34421727487828674	1.2074276264502521
7	0.24601636937265584	1.0067908064327387
8	0.16777630155654757	0.7846894247619397
9	0.1327933198236207	0.6987083507928745
>10	0.719683025974018	7.7894164310571155
>50	0.07691902106440267	3.179968716012872
>100	0.08091886142142074	9.769027929023851
>500	0.008192198413318518	3.153648222216427
>1k	0.0025078158408117913	2.0431027053770334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3397	0.33073670456946297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2490	0.2424299070880079	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1409	0.13718222453293297	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1298	0.12637510819286515	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1265	0.12316218171338554	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1199	0.1167363287544263	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1163	0.11323131804953943	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1151	0.11206298114791048	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1149	0.11186825833097232	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1099	0.10700018790751833	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1091	0.1062212966397657	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1080	0.10515032114660583	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1062	0.1033978157941624	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1041	0.10135322621631174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.736140846907947E-5	0.0
10	0.0	0.0	0.0	9.736140846907947E-5	0.0
11	0.0	0.0	0.0	9.736140846907947E-5	0.0
12	0.0	0.0	0.0	9.736140846907947E-5	9.736140846907947E-5
13	0.0	0.0	0.0	9.736140846907947E-5	9.736140846907947E-5
14	0.0	0.0	0.0	9.736140846907947E-5	9.736140846907947E-5
15	0.0	0.0	0.0	9.736140846907947E-5	2.920842254072384E-4
16	0.0	0.0	0.0	1.9472281693815895E-4	2.920842254072384E-4
17	0.0	0.0	0.0	1.9472281693815895E-4	2.920842254072384E-4
18	0.0	0.0	0.0	1.9472281693815895E-4	2.920842254072384E-4
19	0.0	0.0	0.0	2.920842254072384E-4	2.920842254072384E-4
20	0.0	0.0	0.0	2.920842254072384E-4	2.920842254072384E-4
21	0.0	0.0	0.0	5.841684508144768E-4	3.894456338763179E-4
22	0.0	0.0	0.0	7.788912677526358E-4	3.894456338763179E-4
23	0.0	0.0	0.0	0.0013630597185671127	4.868070423453974E-4
24	0.0	0.0	0.0	0.0025313966201960664	4.868070423453974E-4
25	0.0	0.0	0.0	0.0035050107048868614	4.868070423453974E-4
26	0.0	0.0	0.0	0.004673347606515815	5.841684508144768E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	260	0.0	20.30658	29
TCTAGCG	275	0.0	19.198948	28
TAGCGGC	270	0.0	18.961926	30
GCTCGTT	45	0.00884996	17.77767	12
ACTAATC	55	0.0013650124	17.45359	27
TTCGGAA	305	0.0	17.310528	31
TAAACGC	270	0.0	17.184244	28
TTTCGGA	315	0.0	16.760988	30
GTTTTCG	325	0.0	16.737545	28
TCCAATA	230	0.0	16.695549	2
GTAAACG	280	0.0	16.570522	27
CGCTTCG	275	0.0	16.290016	32
CGCCGGT	355	0.0	16.228413	7
TTAGAGT	160	1.8189894E-11	16.000683	4
TAAGACT	100	1.3134522E-6	16.000683	4
GTCCTAG	130	4.798494E-9	15.999903	1
TTTTCGG	330	0.0	15.999124	29
AACGCTT	285	0.0	15.718438	30
GTCCTAC	155	1.891749E-10	15.483777	1
AAACGCT	300	0.0	15.46582	29
>>END_MODULE