FastQCFastQC Report
Thu 2 Feb 2017
SRR4062973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196650
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32700.2732628588141896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22010.18393013830276186No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21140.17665984205908158No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20300.16964024568587305No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19600.16379058204153263No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18290.1528433543642669No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18110.1513391551414365No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18050.15083775540049305No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18020.1505870555300213No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17600.14707725734341703No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16460.13755066226549117No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15340.12819120043454643No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15240.12735553419964066No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15240.12735553419964066No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14890.12443070237747045No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14350.11991810470897922No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14250.11908243847407346No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14230.11891530522709229No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14090.11774537249822421No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13880.11599047340492208No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA13580.11348347470020474No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13350.11156144235992145No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13220.11047507625454393No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13190.11022437638407219No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13020.10880374378473237No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT12800.10696527806793966No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12800.10696527806793966No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12580.10512681235114694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12390.10353904650482597No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12300.10278694689341078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGG400.00448294120.00049810
ACCGTCG3100.018.06278
CGACGAG450.00885560717.7759928
ACCGTAA550.001365609717.452798
TACCGTC3150.017.2681057
GTATTAG3550.017.1458171
AAGACGG5150.017.0835195
CGCAAGA5050.016.7981132
CTAGCGG4250.016.56373229
TCTAGCG4300.016.37044728
CCGTCGT3450.016.2322869
ATACCGT3450.016.2282166
GCGCAAG5350.016.1675011
CGTATAT2300.016.00039910
CGAGCCG4900.016.00039715
AGCGTAT2300.015.9983928
CATCGTT3100.015.99839128
TACACTA600.00244566915.9963865
TAGCGGC4550.015.82324630
GTCGTAG3450.015.76850611
Produced by FastQC (version 0.11.5)