##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062973_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1196650
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25655371244725	32.0	32.0	32.0	32.0	32.0
2	30.922402540425352	32.0	32.0	32.0	32.0	32.0
3	30.985583086115405	32.0	32.0	32.0	32.0	32.0
4	31.03276563740442	32.0	32.0	32.0	32.0	32.0
5	30.982665775289348	32.0	32.0	32.0	32.0	32.0
6	34.62604103121213	36.0	36.0	36.0	32.0	36.0
7	34.54514352567585	36.0	36.0	36.0	32.0	36.0
8	34.53445869719634	36.0	36.0	36.0	32.0	36.0
9	34.64609451385117	36.0	36.0	36.0	32.0	36.0
10	34.40995947018761	36.0	36.0	36.0	32.0	36.0
11	34.633373166757195	36.0	36.0	36.0	32.0	36.0
12	34.47232524129863	36.0	36.0	36.0	32.0	36.0
13	34.55109096226967	36.0	36.0	36.0	32.0	36.0
14	34.445005640747084	36.0	36.0	36.0	32.0	36.0
15	34.40081059624786	36.0	36.0	36.0	32.0	36.0
16	34.42259474365938	36.0	36.0	36.0	32.0	36.0
17	34.34651234696862	36.0	36.0	36.0	32.0	36.0
18	34.36754773743367	36.0	36.0	36.0	32.0	36.0
19	34.35513809384532	36.0	36.0	36.0	32.0	36.0
20	34.3296360673547	36.0	36.0	36.0	32.0	36.0
21	34.31233694062591	36.0	36.0	36.0	32.0	36.0
22	34.29543642669118	36.0	36.0	36.0	32.0	36.0
23	34.24657000793883	36.0	36.0	36.0	32.0	36.0
24	34.22911294029165	36.0	36.0	36.0	32.0	36.0
25	34.209458070446665	36.0	36.0	36.0	32.0	36.0
26	34.16359921447374	36.0	36.0	36.0	32.0	36.0
27	34.150190114068444	36.0	36.0	36.0	32.0	36.0
28	34.14522959929804	36.0	36.0	36.0	32.0	36.0
29	34.118667112355325	36.0	36.0	36.0	32.0	36.0
30	34.0793799356537	36.0	36.0	36.0	32.0	36.0
31	34.10136297162913	36.0	36.0	36.0	32.0	36.0
32	34.061217565704254	36.0	36.0	36.0	32.0	36.0
33	34.011967576150084	36.0	36.0	36.0	32.0	36.0
34	34.01260853215226	36.0	36.0	36.0	32.0	36.0
35	33.97454309948606	36.0	36.0	36.0	32.0	36.0
36	33.92506413738353	36.0	36.0	36.0	32.0	36.0
37	33.91761250156687	36.0	36.0	36.0	32.0	36.0
38	33.316709982033174	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	2.0
10	6.0
11	8.0
12	5.0
13	4.0
14	135.0
15	282.0
16	374.0
17	439.0
18	592.0
19	758.0
20	1042.0
21	1524.0
22	2268.0
23	3293.0
24	4930.0
25	7228.0
26	10830.0
27	15565.0
28	21801.0
29	29596.0
30	40209.0
31	54567.0
32	75250.0
33	112764.0
34	254185.0
35	558988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77895291725586	17.22231054362287	11.305632023294537	25.693104515826732
2	16.98677188291432	19.637161134536164	36.89215752671585	26.483909455833675
3	18.91567523746244	23.815362242531375	28.330094734246824	28.93886778575936
4	12.44603486862008	15.085082307793876	35.982771271412645	36.4861115521734
5	14.778388930393785	36.34408170837491	33.320296896492366	15.557232464738945
6	34.45638894042181	35.458606806022836	16.770540326880297	13.314463926675057
7	30.67563615092132	30.34162035682948	20.74474574854803	18.237997743701165
8	28.75999147616879	31.90612086291256	19.524086090695235	19.809801570223417
9	27.672882919622143	13.952223210167066	18.586083794064752	39.788810076146035
10	16.19445716520536	25.84770407907448	31.04131220312808	26.916526552592085
11	38.076264175225425	21.11763870202151	21.76570869859522	19.040388424157843
12	25.07188956959855	23.77883835146625	28.04190445310026	23.10736762583495
13	29.36242410059466	18.86216709779061	25.410431388609133	26.364977413005597
14	23.98635745572065	19.839689240157753	24.626865047958674	31.54708825616292
15	25.41828579930473	26.823953439192355	22.375333404102907	25.382427357400005
16	26.253358684523935	25.49758676166399	23.41472179854161	24.834332755270474
17	24.17002112977426	25.777990264255912	24.81820704504119	25.23378156092864
18	25.720047334588013	23.955864508084655	26.21729450738266	24.106793649944677
19	25.52870355745099	25.061029167805348	25.260685014278444	24.149582260465216
20	25.792400874154364	23.687076137507994	25.05463464776844	25.465888340569204
21	27.64728938393228	23.79378441599468	24.104505115860047	24.454421084213
22	25.945908903252118	23.90015159948319	24.958840823885524	25.195098673379164
23	24.113340035987065	23.78464256552847	25.669826608532425	26.43219078995204
24	24.82672102607563	25.11155630553264	24.97406979300131	25.087652875390415
25	25.073341479600618	24.26010775137949	25.051861410931263	25.614689358088626
26	24.61905195737628	25.398040659532622	25.70378536688111	24.279122016209985
27	25.653692410423645	24.525782061578756	24.60099837114903	25.21952715684857
28	24.43334430904025	24.380276487528644	25.814528381673803	25.37185082175731
29	24.20523123698856	24.93174085678492	25.882563351254404	24.980464554972116
30	24.31243951724103	25.043497445308542	26.049078155006345	24.59498488244408
31	25.02546831190429	24.97298562158142	24.478495050497877	25.523051016016414
32	24.68704322601835	24.757745257072084	24.486386516136527	26.068825000773042
33	24.173562179965586	24.28120489640642	25.299882746326734	26.24535017730126
34	25.30179791658309	24.590637996282478	25.47806260948637	24.629501477648066
35	26.254252162647834	24.229177984871914	25.385013999749255	24.131555852730997
36	24.4444370153006	25.351674775112855	24.758188332696747	25.445699876889798
37	25.81985058448228	25.023380127017397	24.72969731615364	24.427071972346678
38	24.49668636185096	24.763950291249152	25.320457641425076	25.418905705474813
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	139.5
2	152.0
3	152.0
4	438.0
5	724.0
6	724.0
7	956.5
8	1189.0
9	1193.0
10	1197.0
11	1197.0
12	1518.5
13	1840.0
14	2355.0
15	2870.0
16	2870.0
17	4128.5
18	5387.0
19	5387.0
20	6470.5
21	7554.0
22	8185.5
23	8817.0
24	8817.0
25	10119.5
26	11422.0
27	11422.0
28	14808.0
29	18194.0
30	21988.0
31	25782.0
32	25782.0
33	33417.0
34	41052.0
35	41052.0
36	45126.0
37	49200.0
38	56242.0
39	63284.0
40	63284.0
41	67470.5
42	71657.0
43	82114.5
44	92572.0
45	92572.0
46	95286.0
47	98000.0
48	98000.0
49	104156.5
50	110313.0
51	111034.0
52	111755.0
53	111755.0
54	106520.0
55	101285.0
56	101285.0
57	98056.5
58	94828.0
59	85060.5
60	75293.0
61	75293.0
62	73137.5
63	70982.0
64	59108.0
65	47234.0
66	47234.0
67	39854.5
68	32475.0
69	32475.0
70	26161.0
71	19847.0
72	15442.0
73	11037.0
74	11037.0
75	8234.0
76	5431.0
77	5431.0
78	5448.0
79	5465.0
80	4316.5
81	3168.0
82	3168.0
83	3241.5
84	3315.0
85	3315.0
86	2184.0
87	1053.0
88	921.5
89	790.0
90	790.0
91	571.5
92	353.0
93	312.5
94	272.0
95	272.0
96	234.0
97	196.0
98	196.0
99	367.0
100	538.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12702126770567834
2	0.0532319391634981
3	0.01211716040613379
4	0.0036769314335854263
5	3.3426649396231143E-4
6	3.3426649396231143E-4
7	0.0
8	4.178331174528893E-4
9	1.6713324698115572E-4
10	5.013997409434672E-4
11	0.0016713324698115573
12	0.0019220323402832906
13	0.013788492875945347
14	0.008273095725567208
15	0.023315087953871223
16	0.010445827936322233
17	0.01955458989679522
18	0.005181130656415828
19	0.0076045627376425855
20	0.005431830526887561
21	0.006434630008774496
22	0.006351063385283917
23	0.009108761960472988
24	0.014122759369907659
25	0.01604479171019095
26	0.018969623532361174
27	0.008440228972548364
28	0.005682530397359295
29	0.008941628713491832
30	0.00233986545773618
31	0.005431830526887561
32	0.006601763255755651
33	0.008273095725567208
34	0.012869260017548992
35	0.01671332469811557
36	0.013454226381983035
37	0.010111561442359921
38	0.006685329879246229
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1196650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.78221723622404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.98607239360558	38.82319597298367
2	13.315832794057346	13.257833616699982
3	4.137782355126507	6.179639402373676
4	1.667321434144151	3.320118313487069
5	0.8233692839197528	2.0494574278863684
6	0.4568079085612204	1.3644546323531908
7	0.2854747453197079	0.9948096050873
8	0.20014742517683254	0.797102607953918
9	0.13383609046818964	0.5996391596760917
>10	0.7619817962987816	7.007479642467882
>50	0.0979957394153025	3.4250444719795756
>100	0.11112026019824355	11.951737260398117
>500	0.014669896307812439	4.822162273509455
>1k	0.0075878774005926405	5.407325613143796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3270	0.2732628588141896	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2201	0.18393013830276186	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2114	0.17665984205908158	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2030	0.16964024568587305	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1960	0.16379058204153263	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1829	0.1528433543642669	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1811	0.1513391551414365	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1805	0.15083775540049305	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1802	0.1505870555300213	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1760	0.14707725734341703	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1646	0.13755066226549117	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1534	0.12819120043454643	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1524	0.12735553419964066	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1524	0.12735553419964066	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1489	0.12443070237747045	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1435	0.11991810470897922	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1425	0.11908243847407346	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1423	0.11891530522709229	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1409	0.11774537249822421	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1388	0.11599047340492208	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1358	0.11348347470020474	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1335	0.11156144235992145	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1322	0.11047507625454393	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1319	0.11022437638407219	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1302	0.10880374378473237	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1280	0.10696527806793966	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1280	0.10696527806793966	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1258	0.10512681235114694	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1239	0.10353904650482597	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1230	0.10278694689341078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.356662349057786E-5
7	0.0	0.0	0.0	0.0	8.356662349057786E-5
8	0.0	0.0	0.0	1.6713324698115572E-4	8.356662349057786E-5
9	0.0	0.0	0.0	1.6713324698115572E-4	8.356662349057786E-5
10	0.0	0.0	0.0	1.6713324698115572E-4	8.356662349057786E-5
11	0.0	0.0	0.0	1.6713324698115572E-4	8.356662349057786E-5
12	0.0	0.0	0.0	1.6713324698115572E-4	3.3426649396231143E-4
13	0.0	0.0	0.0	1.6713324698115572E-4	3.3426649396231143E-4
14	0.0	0.0	0.0	1.6713324698115572E-4	3.3426649396231143E-4
15	0.0	0.0	0.0	1.6713324698115572E-4	4.178331174528893E-4
16	0.0	0.0	0.0	1.6713324698115572E-4	4.178331174528893E-4
17	0.0	0.0	0.0	1.6713324698115572E-4	5.013997409434672E-4
18	0.0	0.0	0.0	1.6713324698115572E-4	5.013997409434672E-4
19	0.0	0.0	0.0	1.6713324698115572E-4	5.013997409434672E-4
20	0.0	0.0	0.0	2.506998704717336E-4	5.84966364434045E-4
21	0.0	0.0	0.0	4.178331174528893E-4	5.84966364434045E-4
22	0.0	0.0	0.0	5.84966364434045E-4	5.84966364434045E-4
23	0.0	0.0	0.0	9.192328583963565E-4	6.685329879246229E-4
24	0.0	0.0	0.0	0.0014206325993398237	6.685329879246229E-4
25	0.0	0.0	0.0	0.0017548990933021352	6.685329879246229E-4
26	0.0	0.0	0.0	0.002506998704717336	6.685329879246229E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGG	40	0.004482941	20.000498	10
ACCGTCG	310	0.0	18.0627	8
CGACGAG	45	0.008855607	17.77599	28
ACCGTAA	55	0.0013656097	17.45279	8
TACCGTC	315	0.0	17.268105	7
GTATTAG	355	0.0	17.145817	1
AAGACGG	515	0.0	17.083519	5
CGCAAGA	505	0.0	16.798113	2
CTAGCGG	425	0.0	16.563732	29
TCTAGCG	430	0.0	16.370447	28
CCGTCGT	345	0.0	16.232286	9
ATACCGT	345	0.0	16.228216	6
GCGCAAG	535	0.0	16.167501	1
CGTATAT	230	0.0	16.000399	10
CGAGCCG	490	0.0	16.000397	15
AGCGTAT	230	0.0	15.998392	8
CATCGTT	310	0.0	15.998391	28
TACACTA	60	0.002445669	15.996386	5
TAGCGGC	455	0.0	15.823246	30
GTCGTAG	345	0.0	15.768506	11
>>END_MODULE