FastQCFastQC Report
Thu 2 Feb 2017
SRR4062973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196650
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37850.3162996699118372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28680.23966907617097732No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22220.185685037396064No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21120.17649270881210044No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20100.1679689132160615No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19100.15961225086700373No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18820.15727238540926752No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18760.15677098566832406No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17140.14323319266285045No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16650.13913842811181215No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16470.13763422888898175No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16310.13629716291313249No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16210.1354614966782267No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15960.13337233109096228No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15840.13236953160907533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15780.13186813186813187No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15440.12902686666945223No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15380.12852546692850877No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15260.12752266744662183No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14940.12484853549492334No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14550.12158943717879078No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14110.11791250574520536No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13850.11573977353445035No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13390.11189570885388375No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13390.11189570885388375No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13230.11055864287803452No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13070.10922157690218526No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13020.10880374378473237No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT12990.10855304391426066No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12590.10521037897463753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC4150.018.5084157
ATACCGT4550.017.9363846
TCGATCT450.00885241117.77710530
AACGCTT2900.017.65450330
CGCTTCG3000.017.59933532
ACCGTCG4450.017.2606588
TAAACGC3000.017.06602128
CGTCGTA4700.017.02276610
CATCGTT3950.017.01201428
GCACCGT854.978143E-616.9433676
CCGTCGT4600.016.6971139
ACGCTTC3100.016.51550331
AAGACGG5150.016.467465
ACCCGTT1950.016.40963630
ATCGTTT4200.016.38033129
CAAGACG5300.016.301954
AAACGCT3350.016.23819229
TCTTATA1203.1099262E-816.0000632
TCGTTTA4200.015.99939430
GTCGTAG4950.015.8397711
Produced by FastQC (version 0.11.5)