##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1196650
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18815944511762	32.0	32.0	32.0	32.0	32.0
2	31.279516984916224	32.0	32.0	32.0	32.0	32.0
3	31.36836585467764	32.0	32.0	32.0	32.0	32.0
4	31.471013245309823	32.0	32.0	32.0	32.0	32.0
5	31.389249989554173	32.0	32.0	32.0	32.0	32.0
6	34.906961935403	36.0	36.0	36.0	36.0	36.0
7	34.9335862616471	36.0	36.0	36.0	36.0	36.0
8	34.879457652613546	36.0	36.0	36.0	32.0	36.0
9	34.98655663727907	36.0	36.0	36.0	36.0	36.0
10	34.82939539547905	36.0	36.0	36.0	32.0	36.0
11	35.01060460452096	36.0	36.0	36.0	36.0	36.0
12	34.89237872393766	36.0	36.0	36.0	32.0	36.0
13	34.95760665190323	36.0	36.0	36.0	36.0	36.0
14	34.9000025069987	36.0	36.0	36.0	32.0	36.0
15	34.86391258931183	36.0	36.0	36.0	32.0	36.0
16	34.883165503697825	36.0	36.0	36.0	32.0	36.0
17	34.836786863326786	36.0	36.0	36.0	32.0	36.0
18	34.839184389754735	36.0	36.0	36.0	32.0	36.0
19	34.83084193373167	36.0	36.0	36.0	32.0	36.0
20	34.80959094137801	36.0	36.0	36.0	32.0	36.0
21	34.81269711277316	36.0	36.0	36.0	32.0	36.0
22	34.79265700079388	36.0	36.0	36.0	32.0	36.0
23	34.72426106213179	36.0	36.0	36.0	32.0	36.0
24	34.7058797476288	36.0	36.0	36.0	32.0	36.0
25	34.674362595579325	36.0	36.0	36.0	32.0	36.0
26	34.61653031379267	36.0	36.0	36.0	32.0	36.0
27	34.60036518614466	36.0	36.0	36.0	32.0	36.0
28	34.57284001169933	36.0	36.0	36.0	32.0	36.0
29	34.52534408557222	36.0	36.0	36.0	32.0	36.0
30	34.50729035223332	36.0	36.0	36.0	32.0	36.0
31	34.50428696778506	36.0	36.0	36.0	32.0	36.0
32	34.471339155141436	36.0	36.0	36.0	32.0	36.0
33	34.43560773826933	36.0	36.0	36.0	32.0	36.0
34	34.42154013287093	36.0	36.0	36.0	32.0	36.0
35	34.37428654995195	36.0	36.0	36.0	32.0	36.0
36	34.34727948857226	36.0	36.0	36.0	32.0	36.0
37	34.33590189278402	36.0	36.0	36.0	32.0	36.0
38	33.89120879120879	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	18.0
22	98.0
23	267.0
24	786.0
25	1978.0
26	4184.0
27	8053.0
28	14116.0
29	22606.0
30	34317.0
31	49592.0
32	71432.0
33	113316.0
34	274368.0
35	601512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.183782148462505	18.444989712157707	12.041706294220589	26.3295218451592
2	15.97835624451594	20.68123511469519	36.1276898006936	27.212718840095267
3	17.868883967743283	24.437554840596665	28.702377470438307	28.99118372122174
4	11.923546503611334	15.945110053156775	36.04691099896461	36.084432444267286
5	14.224400157774047	36.974197926207204	32.942853704681745	15.858548211337
6	34.53371417492863	35.9889457885694	16.262593511207974	13.214746525293988
7	30.395088263510285	30.58570468744255	20.781201426656548	18.238005622390617
8	28.67770383821805	32.140949990097134	19.330315395909988	19.851030775774824
9	27.484616741030578	13.80720094468211	18.443614495488443	40.264567818798874
10	16.02691543049396	26.02468417740718	31.000712831042332	26.947687561056526
11	38.2741203292038	21.01808147192341	21.63458645611173	19.07321174276106
12	24.83152884232905	23.9441304180692	28.041840346836654	23.1825003927651
13	29.436284156841314	18.860668416553224	25.3881463988187	26.314901027786764
14	23.877324196715833	19.80620900012535	24.649646931015752	31.666819872143066
15	25.47570300422011	26.898842602264654	22.239418376300506	25.386036017214725
16	26.2716134806447	25.378703362473036	23.33466204375719	25.01502111312507
17	24.27017089374504	25.775707182551287	24.782852128859734	25.171269794843937
18	25.67592863410354	23.99005557180462	26.211590690678143	24.122425103413697
19	25.641749885095894	24.956670705720136	25.27088121004471	24.130698199139264
20	25.822588058329504	23.592946976977395	25.10750846112063	25.476956503572474
21	27.55726402874692	23.910082313124136	24.10989010989011	24.422763548238834
22	25.921614507165835	23.97543141269377	24.95867630468391	25.14427777545648
23	24.031608266083037	23.601657628928784	25.73820727715479	26.628526827833394
24	24.752517444532653	24.987005390047216	25.068232148080057	25.192245017340078
25	25.05674183218457	24.158482694994685	25.121506073632343	25.663269399188398
26	24.674236137747997	25.385221564552346	25.555864752320858	24.384677545378803
27	25.776731522777357	24.350833034582404	24.641895827431433	25.2305396152088
28	24.46412902686667	24.26365269711277	25.986211507124057	25.286006768896502
29	24.38515856766807	24.649479797768773	25.889274223874985	25.07608741068817
30	24.5029039401663	24.744327915430578	26.138219195253416	24.614548949149707
31	25.11143609242469	24.711653365645763	24.59006392846697	25.586846613462583
32	24.8487861947938	24.577946768060837	24.571679271299043	26.001587765846324
33	24.337024192537502	24.15050348890653	25.418459867129066	26.094012451426902
34	25.378013621359628	24.33560355993816	25.65712614381816	24.62925667488405
35	26.402791125224585	23.996991601554342	25.463418710566998	24.13679856265408
36	24.61471608239669	25.08895667070572	24.974219696653158	25.322107550244432
37	25.932060335102157	24.775163999498602	24.830568670872854	24.462206994526387
38	24.64557276193771	24.382755511432343	25.413216406816037	25.558455319813916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	77.0
2	100.0
3	100.0
4	382.5
5	665.0
6	665.0
7	914.0
8	1163.0
9	1224.5
10	1286.0
11	1286.0
12	1596.0
13	1906.0
14	2459.5
15	3013.0
16	3013.0
17	4530.0
18	6047.0
19	6047.0
20	6959.5
21	7872.0
22	8397.5
23	8923.0
24	8923.0
25	10125.5
26	11328.0
27	11328.0
28	14730.0
29	18132.0
30	21903.0
31	25674.0
32	25674.0
33	33430.0
34	41186.0
35	41186.0
36	44750.5
37	48315.0
38	55950.0
39	63585.0
40	63585.0
41	67238.0
42	70891.0
43	81657.5
44	92424.0
45	92424.0
46	94930.0
47	97436.0
48	97436.0
49	103938.0
50	110440.0
51	111176.0
52	111912.0
53	111912.0
54	106437.0
55	100962.0
56	100962.0
57	98208.5
58	95455.0
59	85602.0
60	75749.0
61	75749.0
62	74052.5
63	72356.0
64	60015.0
65	47674.0
66	47674.0
67	40221.0
68	32768.0
69	32768.0
70	26481.0
71	20194.0
72	15655.0
73	11116.0
74	11116.0
75	8146.5
76	5177.0
77	5177.0
78	5356.0
79	5535.0
80	4297.0
81	3059.0
82	3059.0
83	2940.5
84	2822.0
85	2822.0
86	1785.5
87	749.0
88	639.0
89	529.0
90	529.0
91	311.0
92	93.0
93	65.0
94	37.0
95	37.0
96	24.0
97	11.0
98	11.0
99	11.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007688129361133163
2	0.0
3	0.0
4	8.356662349057786E-5
5	1.6713324698115572E-4
6	1.6713324698115572E-4
7	5.013997409434672E-4
8	0.002256298834245602
9	0.004763297538962938
10	0.0010863661053775123
11	0.005515397150378139
12	5.013997409434672E-4
13	8.356662349057786E-5
14	0.0
15	0.0
16	8.356662349057786E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	8.356662349057786E-5
24	0.0
25	1.6713324698115572E-4
26	8.356662349057786E-5
27	8.356662349057786E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.356662349057786E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1196650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.64429515812316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16682109878863	36.28914505686648
2	14.324504697698275	13.649618596221167
3	4.531668276507861	6.477244227739315
4	1.8485370375175547	3.522889769048359
5	0.9082189017753675	2.163572471218604
6	0.47681274156037273	1.3630444196433777
7	0.32650256115668974	1.0889189075542738
8	0.21169581731771786	0.8068878403220375
9	0.1459619262439358	0.6258827786232853
>10	0.813266533129414	7.213830818216698
>50	0.10388358462332936	3.5044746829912974
>100	0.11814512769733579	12.36233095135322
>500	0.016046575988915106	5.223825243500332
>1k	0.007935119994518458	5.708334236701567
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3785	0.3162996699118372	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2868	0.23966907617097732	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2222	0.185685037396064	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2112	0.17649270881210044	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2010	0.1679689132160615	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1910	0.15961225086700373	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1882	0.15727238540926752	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1876	0.15677098566832406	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1714	0.14323319266285045	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1665	0.13913842811181215	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1647	0.13763422888898175	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1631	0.13629716291313249	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1621	0.1354614966782267	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1596	0.13337233109096228	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1584	0.13236953160907533	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1578	0.13186813186813187	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1544	0.12902686666945223	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1538	0.12852546692850877	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1526	0.12752266744662183	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1494	0.12484853549492334	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1455	0.12158943717879078	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1411	0.11791250574520536	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1385	0.11573977353445035	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1339	0.11189570885388375	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1339	0.11189570885388375	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1323	0.11055864287803452	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1307	0.10922157690218526	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1302	0.10880374378473237	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1299	0.10855304391426066	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1259	0.10521037897463753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.356662349057786E-5	0.0
5	0.0	0.0	0.0	8.356662349057786E-5	0.0
6	0.0	0.0	0.0	8.356662349057786E-5	0.0
7	0.0	0.0	0.0	8.356662349057786E-5	0.0
8	0.0	0.0	0.0	8.356662349057786E-5	0.0
9	0.0	0.0	0.0	8.356662349057786E-5	0.0
10	0.0	0.0	0.0	8.356662349057786E-5	0.0
11	0.0	0.0	0.0	8.356662349057786E-5	0.0
12	0.0	0.0	0.0	8.356662349057786E-5	0.0
13	0.0	0.0	0.0	8.356662349057786E-5	8.356662349057786E-5
14	0.0	0.0	0.0	8.356662349057786E-5	8.356662349057786E-5
15	0.0	0.0	0.0	8.356662349057786E-5	1.6713324698115572E-4
16	0.0	0.0	0.0	8.356662349057786E-5	1.6713324698115572E-4
17	0.0	0.0	0.0	8.356662349057786E-5	1.6713324698115572E-4
18	0.0	0.0	0.0	1.6713324698115572E-4	1.6713324698115572E-4
19	0.0	0.0	0.0	1.6713324698115572E-4	1.6713324698115572E-4
20	0.0	0.0	0.0	1.6713324698115572E-4	2.506998704717336E-4
21	0.0	0.0	0.0	3.3426649396231143E-4	3.3426649396231143E-4
22	0.0	0.0	0.0	5.013997409434672E-4	3.3426649396231143E-4
23	0.0	0.0	0.0	8.356662349057786E-4	5.013997409434672E-4
24	0.0	0.0	0.0	0.00116993272886809	5.013997409434672E-4
25	0.0	0.0	0.0	0.0014206325993398237	5.013997409434672E-4
26	0.0	0.0	0.0	0.0020891655872644467	5.013997409434672E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	415	0.0	18.508415	7
ATACCGT	455	0.0	17.936384	6
TCGATCT	45	0.008852411	17.777105	30
AACGCTT	290	0.0	17.654503	30
CGCTTCG	300	0.0	17.599335	32
ACCGTCG	445	0.0	17.260658	8
TAAACGC	300	0.0	17.066021	28
CGTCGTA	470	0.0	17.022766	10
CATCGTT	395	0.0	17.012014	28
GCACCGT	85	4.978143E-6	16.943367	6
CCGTCGT	460	0.0	16.697113	9
ACGCTTC	310	0.0	16.515503	31
AAGACGG	515	0.0	16.46746	5
ACCCGTT	195	0.0	16.409636	30
ATCGTTT	420	0.0	16.380331	29
CAAGACG	530	0.0	16.30195	4
AAACGCT	335	0.0	16.238192	29
TCTTATA	120	3.1099262E-8	16.000063	2
TCGTTTA	420	0.0	15.999394	30
GTCGTAG	495	0.0	15.83977	11
>>END_MODULE