FastQCFastQC Report
Thu 2 Feb 2017
SRR4062972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314466
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50460.21802005300574734No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT50450.21797684649504465No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC47810.20657032766953587No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT42120.18198582307970823No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA34850.1505746897988564No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC34060.14716137545334432No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC34000.1469021363891282No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG33210.14348882204361613No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC32630.14098284442286038No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG32140.13886572539842884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31570.1364029542883758No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG30990.1338969766676201No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC30050.12983556466156773No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG29690.12828013027627108No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG28950.1250828484842724No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27500.1188179044323831No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC27410.11842904583605894No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG27090.11704643749357302No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25760.11129997157011595No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25240.10905323301357635No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA25200.10888040697076562No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC25050.10823230931022534No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT25050.10823230931022534No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG24760.10697932049984749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8500.019.9515828
CTAGCGG8550.019.64778329
TAGCGGC9050.018.5626730
GCTTACG450.00885748817.7761828
CAAGACG9100.017.4035264
ATACCGT6000.017.3302786
AGCGGCG9900.017.29212431
CGGTCCA10000.017.2795710
TATACCG652.1047407E-417.2273625
AAGACGG9550.016.7506145
TTTATAC1750.016.4560223
CGCAAGA9750.016.4158762
TACCGTC6400.016.248547
GTATCAA40350.016.2341731
GTCCTAT7400.016.0158651
ACCGTCG6300.015.9985638
GCGCAAG10200.015.8588471
CCGGTCC11200.015.7138939
CGCCGGT10250.015.4522717
TATACTG2600.015.3815745
Produced by FastQC (version 0.11.5)