##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062972_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2314466 Sequences flagged as poor quality 0 Sequence length 38 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28666439688464 32.0 32.0 32.0 32.0 32.0 2 30.91354074762818 32.0 32.0 32.0 32.0 32.0 3 30.963779550012834 32.0 32.0 32.0 32.0 32.0 4 31.02469468119212 32.0 32.0 32.0 32.0 32.0 5 30.9310908002105 32.0 32.0 32.0 32.0 32.0 6 34.59442134816411 36.0 36.0 36.0 32.0 36.0 7 34.53232927163329 36.0 36.0 36.0 32.0 36.0 8 34.506636520043934 36.0 36.0 36.0 32.0 36.0 9 34.65832982640488 36.0 36.0 36.0 32.0 36.0 10 34.37330770899205 36.0 36.0 36.0 32.0 36.0 11 34.62815958411141 36.0 36.0 36.0 32.0 36.0 12 34.45007660514347 36.0 36.0 36.0 32.0 36.0 13 34.54847511261777 36.0 36.0 36.0 32.0 36.0 14 34.435688405014375 36.0 36.0 36.0 32.0 36.0 15 34.37478796404873 36.0 36.0 36.0 32.0 36.0 16 34.393750869531026 36.0 36.0 36.0 32.0 36.0 17 34.3137043274777 36.0 36.0 36.0 32.0 36.0 18 34.33866991349193 36.0 36.0 36.0 32.0 36.0 19 34.33373659409989 36.0 36.0 36.0 32.0 36.0 20 34.31413984910558 36.0 36.0 36.0 32.0 36.0 21 34.29489653336882 36.0 36.0 36.0 32.0 36.0 22 34.278997401560446 36.0 36.0 36.0 32.0 36.0 23 34.2211408592738 36.0 36.0 36.0 32.0 36.0 24 34.20475781454556 36.0 36.0 36.0 32.0 36.0 25 34.18939703586054 36.0 36.0 36.0 32.0 36.0 26 34.13146358598485 36.0 36.0 36.0 32.0 36.0 27 34.12630084002098 36.0 36.0 36.0 32.0 36.0 28 34.11850076864383 36.0 36.0 36.0 32.0 36.0 29 34.08831670026693 36.0 36.0 36.0 32.0 36.0 30 34.06363670928845 36.0 36.0 36.0 32.0 36.0 31 34.080784509256134 36.0 36.0 36.0 32.0 36.0 32 34.039595310538154 36.0 36.0 36.0 32.0 36.0 33 34.004903506899645 36.0 36.0 36.0 32.0 36.0 34 34.0175915308326 36.0 36.0 36.0 32.0 36.0 35 33.97743712804595 36.0 36.0 36.0 32.0 36.0 36 33.94197711264715 36.0 36.0 36.0 32.0 36.0 37 33.944733688029984 36.0 36.0 36.0 32.0 36.0 38 33.326413954666 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 14.0 10 11.0 11 29.0 12 6.0 13 6.0 14 189.0 15 398.0 16 648.0 17 756.0 18 982.0 19 1247.0 20 1849.0 21 2732.0 22 4261.0 23 6195.0 24 9506.0 25 14096.0 26 20919.0 27 30384.0 28 42326.0 29 57760.0 30 78740.0 31 107718.0 32 148130.0 33 225709.0 34 506609.0 35 1053243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.43908917092753 16.651396783375294 11.041942432972567 25.867571612724618 2 17.658382434950266 18.338859516779568 36.464164825806336 27.538593222463824 3 20.078084657250702 21.8758466913409 27.19930168895342 30.84676696245498 4 12.952837966059514 14.17336817599693 34.49043468642467 38.38335917151889 5 15.63013213887133 35.36861097993095 32.69351498557546 16.30774189562226 6 36.719851092824165 33.93337198882505 15.680188311581697 13.66658860676909 7 32.38142189170202 29.074525182050632 19.346233645255538 19.197819280991816 8 29.511640497169324 31.996450148479365 18.20582139355868 20.286087960792635 9 27.205706032025574 14.086464117162384 18.066523422495845 40.6413064283162 10 16.6771514737779 25.302489565600617 29.868003767617502 28.152355193003984 11 39.267716974308605 20.56179634493632 20.88874467310136 19.28174200765371 12 25.956268806072558 22.790065035957298 27.047863561025437 24.205802596944707 13 29.59663636776116 18.210758524132896 25.044497691391925 27.14810741671402 14 24.017724811711684 19.286340833178496 23.66188906220968 33.03404529290014 15 25.9010540081394 26.629555185141808 21.265345652351478 26.20404515436731 16 27.11509784459719 25.09222285934291 22.390158977215325 25.402520318844573 17 24.92923502958948 25.50408427319298 23.935889313646772 25.630791383570774 18 26.33695939604797 23.75978457938191 25.163933292890118 24.739322731679998 19 26.189558502253806 24.162383161416994 24.585274881778712 25.06278345455048 20 26.50902996131529 23.463770188705173 23.95708343289328 26.070116417086258 21 27.859311030213995 23.30212913052402 23.67606105451914 25.162498784742855 22 26.684293124039677 23.271287857707044 24.464379231257162 25.580039786996117 23 24.79046226851682 23.160827581677264 25.25687465674788 26.79183549305804 24 25.487737213912975 24.36361247902043 24.454617579800153 25.694032727266443 25 25.666005213266864 23.77991232861558 24.467783531884592 26.086298926232963 26 25.20729835405885 25.19748873838835 24.908645253183156 24.686567654369643 27 26.088444442331966 23.919743817647664 24.159903970506708 25.831907769513663 28 25.125921265776075 23.787960557272626 25.293312184062604 25.792805992888695 29 24.786797889238606 24.321676786637145 25.2700675298457 25.621457794278545 30 24.88717402528944 24.525309960875475 25.414350556622544 25.173165457212544 31 25.793187839946906 24.404623018650735 23.851115570281188 25.95107357112117 32 25.558857219893007 23.971912280102888 23.721601281758232 26.747629218245873 33 24.884964669494604 23.86378710696892 24.72763773236618 26.52361049117029 34 26.187565600168348 23.872995049280338 24.659397660280465 25.280041690270842 35 26.63652709903492 23.62389553191425 24.983028127718363 24.75654924133246 36 24.820862022921713 24.69973700790532 24.32279319556569 26.156607773607277 37 26.471252909666077 24.2287737510203 24.161322646589483 25.13865069272414 38 25.194408568884175 23.86002496622128 24.856815825939897 26.08875063895465 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 66.0 1 107.0 2 148.0 3 148.0 4 392.0 5 636.0 6 636.0 7 818.5 8 1001.0 9 1081.0 10 1161.0 11 1161.0 12 1595.5 13 2030.0 14 2883.0 15 3736.0 16 3736.0 17 5586.5 18 7437.0 19 7437.0 20 9097.5 21 10758.0 22 11556.5 23 12355.0 24 12355.0 25 14211.0 26 16067.0 27 16067.0 28 21051.0 29 26035.0 30 32661.0 31 39287.0 32 39287.0 33 52500.5 34 65714.0 35 65714.0 36 74526.5 37 83339.0 38 96972.0 39 110605.0 40 110605.0 41 118261.5 42 125918.0 43 147013.5 44 168109.0 45 168109.0 46 172135.0 47 176161.0 48 176161.0 49 192868.5 50 209576.0 51 218671.5 52 227767.0 53 227767.0 54 220665.0 55 213563.0 56 213563.0 57 208896.5 58 204230.0 59 185213.5 60 166197.0 61 166197.0 62 159234.0 63 152271.0 64 128386.5 65 104502.0 66 104502.0 67 88242.5 68 71983.0 69 71983.0 70 58292.5 71 44602.0 72 34627.0 73 24652.0 74 24652.0 75 18671.0 76 12690.0 77 12690.0 78 12238.0 79 11786.0 80 9188.5 81 6591.0 82 6591.0 83 6679.5 84 6768.0 85 6768.0 86 4543.5 87 2319.0 88 1964.0 89 1609.0 90 1609.0 91 1160.5 92 712.0 93 625.0 94 538.0 95 538.0 96 484.5 97 431.0 98 431.0 99 773.5 100 1116.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11778094817551867 2 0.053403247228518365 3 0.013437224828534962 4 0.0026788036635664557 5 3.024455749187934E-4 6 1.7282604281073906E-4 7 0.0 8 1.296195321080543E-4 9 1.296195321080543E-4 10 2.592390642161086E-4 11 0.0014258148531885973 12 0.0023331515779449774 13 0.013005159721508116 14 0.008468476097726213 15 0.02320189624734172 16 0.01015353001513092 17 0.02022064700885647 18 0.004752716177295324 19 0.0069994547338349325 20 0.004968748730808748 21 0.006092118009078552 22 0.0061353245197812364 23 0.008857334694050378 24 0.013394018317832278 25 0.015986408959993363 26 0.017714669388100755 27 0.007647552394375204 28 0.005314400816430226 29 0.009937497461617496 30 0.0026355971528637706 31 0.0057896724341597585 32 0.00626494405188929 33 0.007647552394375204 34 0.011104073250589984 35 0.014819833171020874 36 0.014171735510480605 37 0.008511682608428899 38 0.0060057049876731826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 2314466.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.57407179782944 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93779664336432 30.62684919272246 2 14.832606519355041 12.629689098079488 3 5.567985829293728 7.11155485396944 4 2.6511831108995083 4.514866404505139 5 1.4224035585605554 3.0278755613822588 6 0.8268581382938796 2.112163064780593 7 0.5739109347082376 1.7103607737879538 8 0.38068754686125966 1.2965935162088662 9 0.2764506424732865 1.0592666551092396 >10 1.3116799801051753 9.852494265402154 >50 0.09305551862697457 2.779480596242576 >100 0.09529004824966086 8.783357944906257 >500 0.017952912341243642 5.272998639870686 >1k 0.011934606499576738 8.784219905155025 >5k 2.0401036751413224E-4 0.43822952787790387 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 5046 0.21802005300574734 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 5045 0.21797684649504465 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 4781 0.20657032766953587 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT 4212 0.18198582307970823 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA 3485 0.1505746897988564 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC 3406 0.14716137545334432 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC 3400 0.1469021363891282 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 3321 0.14348882204361613 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC 3263 0.14098284442286038 No Hit GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG 3214 0.13886572539842884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 3157 0.1364029542883758 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 3099 0.1338969766676201 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC 3005 0.12983556466156773 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 2969 0.12828013027627108 No Hit GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG 2895 0.1250828484842724 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG 2750 0.1188179044323831 No Hit ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC 2741 0.11842904583605894 No Hit ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG 2709 0.11704643749357302 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG 2576 0.11129997157011595 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 2524 0.10905323301357635 No Hit GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA 2520 0.10888040697076562 No Hit CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC 2505 0.10823230931022534 No Hit GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT 2505 0.10823230931022534 No Hit GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG 2476 0.10697932049984749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.296195321080543E-4 2 0.0 0.0 0.0 0.0 1.296195321080543E-4 3 0.0 0.0 0.0 0.0 1.296195321080543E-4 4 0.0 0.0 0.0 0.0 1.296195321080543E-4 5 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 6 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 7 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 8 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 9 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 10 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 11 0.0 0.0 0.0 4.3206510702684765E-5 1.296195321080543E-4 12 0.0 0.0 0.0 4.3206510702684765E-5 4.7527161772953244E-4 13 0.0 0.0 0.0 4.3206510702684765E-5 4.7527161772953244E-4 14 0.0 0.0 0.0 8.641302140536953E-5 4.7527161772953244E-4 15 0.0 0.0 0.0 8.641302140536953E-5 4.7527161772953244E-4 16 0.0 0.0 0.0 8.641302140536953E-5 5.184781284322172E-4 17 0.0 0.0 0.0 1.296195321080543E-4 5.184781284322172E-4 18 0.0 0.0 0.0 1.296195321080543E-4 5.184781284322172E-4 19 0.0 0.0 0.0 1.296195321080543E-4 5.184781284322172E-4 20 0.0 0.0 0.0 2.1603255351342382E-4 5.184781284322172E-4 21 0.0 0.0 0.0 3.456520856214781E-4 5.616846391349019E-4 22 0.0 0.0 0.0 5.184781284322172E-4 5.616846391349019E-4 23 0.0 0.0 0.0 9.505432354590649E-4 5.616846391349019E-4 24 0.0 0.0 0.0 0.001296195321080543 6.048911498375868E-4 25 0.0 0.0 0.0 0.0015986408959993363 6.048911498375868E-4 26 0.0 0.0 0.0 0.0021603255351342384 6.048911498375868E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAGCG 850 0.0 19.95158 28 CTAGCGG 855 0.0 19.647783 29 TAGCGGC 905 0.0 18.56267 30 GCTTACG 45 0.008857488 17.776182 8 CAAGACG 910 0.0 17.403526 4 ATACCGT 600 0.0 17.330278 6 AGCGGCG 990 0.0 17.292124 31 CGGTCCA 1000 0.0 17.27957 10 TATACCG 65 2.1047407E-4 17.227362 5 AAGACGG 955 0.0 16.750614 5 TTTATAC 175 0.0 16.456022 3 CGCAAGA 975 0.0 16.415876 2 TACCGTC 640 0.0 16.24854 7 GTATCAA 4035 0.0 16.234173 1 GTCCTAT 740 0.0 16.015865 1 ACCGTCG 630 0.0 15.998563 8 GCGCAAG 1020 0.0 15.858847 1 CCGGTCC 1120 0.0 15.713893 9 CGCCGGT 1025 0.0 15.452271 7 TATACTG 260 0.0 15.381574 5 >>END_MODULE