FastQCFastQC Report
Thu 2 Feb 2017
SRR4062972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314466
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53560.2314140713235796No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC52360.22622929003925743No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT49560.2141314670425057No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT42850.18513989836100422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36730.15869751381096114No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35770.15454968878350342No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC35210.15213012418415306No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC35060.15148202652361278No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA34750.15014262469182957No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC33620.14526028898242618No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG32730.14141490952988725No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31630.13666219335259192No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30550.13199589019670194No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG30440.13152061857897243No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG29920.12927388002243284No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA27690.11963882813573411No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC27560.11907714349659923No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG27540.11899073047519386No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG27400.11838583932535626No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG26930.11635513332233008No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG26130.1128986124661153No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT25860.11173203667714281No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG25710.11108393901660253No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25230.10901002650287367No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT23780.10274508245098438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2650.022.3404374
TCTAGCG8050.018.68270128
CTAGCGG8050.018.68270129
TAAACGC6750.017.30321328
AAACGCT7100.017.12627429
CAAGACG10050.016.8762364
AACGCTT7100.016.67558330
GTAATAC1354.4383341E-1016.5928383
GTATCAA38900.016.576441
GACGGAC10650.016.2266467
AAGACGG10750.016.22495
TAGCGGC9300.016.17158330
GCGCAAG10600.015.8496351
ACGCTTC7400.015.78333731
ACCGTCG7150.015.6655858
CGCAAGA10550.015.6210842
GTAAACG7550.015.46975927
TACCGTC7400.015.3525757
GCGAAAG11350.015.22423718
ACACGCT10850.015.1898229
Produced by FastQC (version 0.11.5)