##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2314466
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176982941205445	32.0	32.0	32.0	32.0	32.0
2	31.21607100730795	32.0	32.0	32.0	32.0	32.0
3	31.332801173143178	32.0	32.0	32.0	32.0	32.0
4	31.44600698390039	32.0	32.0	32.0	32.0	32.0
5	31.3400641875923	32.0	32.0	32.0	32.0	32.0
6	34.87886968311481	36.0	36.0	36.0	36.0	36.0
7	34.90725765684179	36.0	36.0	36.0	36.0	36.0
8	34.83369727617515	36.0	36.0	36.0	32.0	36.0
9	34.9623757704801	36.0	36.0	36.0	32.0	36.0
10	34.78669636970256	36.0	36.0	36.0	32.0	36.0
11	34.97898521732443	36.0	36.0	36.0	36.0	36.0
12	34.86921994101447	36.0	36.0	36.0	32.0	36.0
13	34.93572513054847	36.0	36.0	36.0	36.0	36.0
14	34.875938985493846	36.0	36.0	36.0	32.0	36.0
15	34.83179532557402	36.0	36.0	36.0	32.0	36.0
16	34.84954239984515	36.0	36.0	36.0	32.0	36.0
17	34.79899769536472	36.0	36.0	36.0	32.0	36.0
18	34.81389313992947	36.0	36.0	36.0	32.0	36.0
19	34.8121484610273	36.0	36.0	36.0	32.0	36.0
20	34.79156142280768	36.0	36.0	36.0	32.0	36.0
21	34.78777437214459	36.0	36.0	36.0	32.0	36.0
22	34.777788483390985	36.0	36.0	36.0	32.0	36.0
23	34.714612355506624	36.0	36.0	36.0	32.0	36.0
24	34.68313943691547	36.0	36.0	36.0	32.0	36.0
25	34.668565448790346	36.0	36.0	36.0	32.0	36.0
26	34.597564189752624	36.0	36.0	36.0	32.0	36.0
27	34.58242290014198	36.0	36.0	36.0	32.0	36.0
28	34.55027423172343	36.0	36.0	36.0	32.0	36.0
29	34.50983250564061	36.0	36.0	36.0	32.0	36.0
30	34.487143902740414	36.0	36.0	36.0	32.0	36.0
31	34.49217054819557	36.0	36.0	36.0	32.0	36.0
32	34.47101534436021	36.0	36.0	36.0	32.0	36.0
33	34.43406945705834	36.0	36.0	36.0	32.0	36.0
34	34.43130337624316	36.0	36.0	36.0	32.0	36.0
35	34.39549425223788	36.0	36.0	36.0	32.0	36.0
36	34.369680090353455	36.0	36.0	36.0	32.0	36.0
37	34.36700647147117	36.0	36.0	36.0	32.0	36.0
38	33.90480655148963	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	10.0
21	51.0
22	172.0
23	545.0
24	1503.0
25	3719.0
26	8173.0
27	15702.0
28	27495.0
29	43877.0
30	65853.0
31	96375.0
32	140199.0
33	228127.0
34	549516.0
35	1133147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73040427260314	17.92384672554747	11.730473408576318	26.61527559327307
2	16.57349902742144	19.353794784628505	35.71273027990042	28.359975908049634
3	18.933913913619815	22.638872206375034	27.616867130474155	30.810346749530993
4	12.372416409335557	15.03536673517788	34.6164531470151	37.97576370847147
5	15.176609001742522	35.93637055334218	32.253572426951735	16.633448017963563
6	36.73227871864878	34.33483274521995	15.300525435057722	13.632363101073553
7	32.111635688198135	29.313527967899166	19.334081384964165	19.24075495893853
8	29.4305447836485	32.17544033995778	17.993825626888984	20.400189249504734
9	27.064233661165733	13.922175740172158	17.778303953432303	41.235286645229806
10	16.452727502902427	25.560231986702632	29.8173685850116	28.169671925383344
11	39.432948820818204	20.536053395346183	20.699122305571436	19.331875478264177
12	25.719617413831912	22.915117528545547	26.957530895989084	24.407734161633453
13	29.600602818018697	18.225393968276872	24.981215945124205	27.192787268580226
14	23.899033297529538	19.20291764925473	23.59632848354653	33.3017205696692
15	25.867953990250882	26.814435813703895	21.111176400949503	26.206433795095712
16	27.224618745420102	25.175850650517788	22.151781146736347	25.44774945732576
17	24.978807206500335	25.60646818747824	23.765741212011758	25.64898339400968
18	26.382629945741265	23.688358351343247	25.001663450662054	24.927348252253438
19	26.20198352449334	24.275750864346247	24.41033914518511	25.111926465975305
20	26.600736411768416	23.35510653429344	23.84541401774751	26.198743036190635
21	27.87650709775261	23.305256290330593	23.607615582866885	25.21062102904991
22	26.697012052461368	23.30560194256556	24.44448285024833	25.552903154724742
23	24.73624539957882	23.095993798990865	25.24638555310046	26.92137524832985
24	25.48345925150769	24.296144337397912	24.437300007863584	25.78309640323081
25	25.59438142292063	23.81781534825543	24.51365650377085	26.074146725053087
26	25.28628658730488	25.082308474013853	24.811360211262738	24.82004472741853
27	26.24860334954154	23.76085887630235	24.185103604892014	25.805434169264096
28	25.13340010179454	23.77049392818905	25.322817444715106	25.773288525301304
29	24.957290364170394	24.187825615066284	25.230355511811364	25.624528508951954
30	24.920219177987494	24.376465240794204	25.4368826329702	25.26643294824811
31	25.773850209940434	24.299255206168507	23.965009639372536	25.96188494451852
32	25.64034209186914	23.914112369764776	23.81292272169909	26.632622816666995
33	24.893474347862533	23.786480337149044	24.724882543100655	26.59516277188777
34	26.126069685188725	23.83081021713	24.73335101919838	25.309769078482898
35	26.6133527128936	23.569454033889457	24.955821342806505	24.861371910410433
36	24.862452073178005	24.650394518649225	24.3699842641888	26.117169143983972
37	26.588249730175338	24.149674266115813	24.147600353602083	25.114475650106765
38	25.274221040283606	23.647581622534798	24.88821390688561	26.18998343029599
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	52.5
2	70.0
3	70.0
4	269.5
5	469.0
6	469.0
7	708.5
8	948.0
9	1077.5
10	1207.0
11	1207.0
12	1614.5
13	2022.0
14	2930.5
15	3839.0
16	3839.0
17	5816.0
18	7793.0
19	7793.0
20	9303.0
21	10813.0
22	11643.5
23	12474.0
24	12474.0
25	14262.0
26	16050.0
27	16050.0
28	20885.5
29	25721.0
30	32180.5
31	38640.0
32	38640.0
33	52545.5
34	66451.0
35	66451.0
36	74643.0
37	82835.0
38	96534.5
39	110234.0
40	110234.0
41	118041.0
42	125848.0
43	147559.5
44	169271.0
45	169271.0
46	172145.0
47	175019.0
48	175019.0
49	192323.5
50	209628.0
51	218306.5
52	226985.0
53	226985.0
54	220569.0
55	214153.0
56	214153.0
57	210324.0
58	206495.0
59	186829.5
60	167164.0
61	167164.0
62	160517.0
63	153870.0
64	129246.5
65	104623.0
66	104623.0
67	88790.5
68	72958.0
69	72958.0
70	58823.5
71	44689.0
72	34653.5
73	24618.0
74	24618.0
75	18557.5
76	12497.0
77	12497.0
78	12054.0
79	11611.0
80	9066.0
81	6521.0
82	6521.0
83	6124.0
84	5727.0
85	5727.0
86	3741.5
87	1756.0
88	1418.0
89	1080.0
90	1080.0
91	649.0
92	218.0
93	145.0
94	72.0
95	72.0
96	50.5
97	29.0
98	29.0
99	31.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008036410990699367
2	0.0
3	0.0
4	1.296195321080543E-4
5	1.296195321080543E-4
6	1.296195321080543E-4
7	3.888585963241629E-4
8	0.0026355971528637706
9	0.0031108687705933033
10	9.937497461617495E-4
11	0.00462309664518727
12	3.024455749187934E-4
13	1.296195321080543E-4
14	0.0
15	0.0
16	8.641302140536953E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	4.3206510702684765E-5
22	4.3206510702684765E-5
23	1.7282604281073906E-4
24	0.0
25	4.3206510702684765E-5
26	8.641302140536953E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.3206510702684765E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2314466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.47992418171516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.72860209108275	28.226085259440147
2	15.840844067784243	12.82472333676557
3	6.055502580755974	7.353788560535469
4	2.859893750488907	4.630731287502078
5	1.5921198752363315	3.2224445918884252
6	0.9458850664068678	2.2973613463659968
7	0.6143752362631234	1.7408904088138202
8	0.41331040831430327	1.3384619193661382
9	0.304252528915705	1.1084507372342554
>10	1.4066492785626747	10.01704710872459
>50	0.10345449901332773	2.915188128078496
>100	0.10168630954603307	8.848093786993966
>500	0.0200975740410633	5.579418471717726
>1k	0.013111786272779261	9.436507392827382
>5k	2.14947315947201E-4	0.4608076637460069
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5356	0.2314140713235796	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5236	0.22622929003925743	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4956	0.2141314670425057	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4285	0.18513989836100422	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3673	0.15869751381096114	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3577	0.15454968878350342	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3521	0.15213012418415306	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3506	0.15148202652361278	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3475	0.15014262469182957	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3362	0.14526028898242618	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3273	0.14141490952988725	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3163	0.13666219335259192	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3055	0.13199589019670194	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	3044	0.13152061857897243	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2992	0.12927388002243284	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2769	0.11963882813573411	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2756	0.11907714349659923	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2754	0.11899073047519386	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2740	0.11838583932535626	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2693	0.11635513332233008	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2613	0.1128986124661153	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2586	0.11173203667714281	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2571	0.11108393901660253	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2523	0.10901002650287367	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2378	0.10274508245098438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.3206510702684765E-5
2	0.0	0.0	0.0	0.0	4.3206510702684765E-5
3	0.0	0.0	0.0	0.0	4.3206510702684765E-5
4	0.0	0.0	0.0	0.0	4.3206510702684765E-5
5	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
6	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
7	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
8	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
9	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
10	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
11	0.0	0.0	0.0	4.3206510702684765E-5	4.3206510702684765E-5
12	0.0	0.0	0.0	4.3206510702684765E-5	3.024455749187934E-4
13	0.0	0.0	0.0	4.3206510702684765E-5	3.888585963241629E-4
14	0.0	0.0	0.0	8.641302140536953E-5	3.888585963241629E-4
15	0.0	0.0	0.0	1.296195321080543E-4	4.7527161772953244E-4
16	0.0	0.0	0.0	1.296195321080543E-4	5.184781284322172E-4
17	0.0	0.0	0.0	1.7282604281073906E-4	5.184781284322172E-4
18	0.0	0.0	0.0	1.7282604281073906E-4	5.184781284322172E-4
19	0.0	0.0	0.0	1.7282604281073906E-4	5.184781284322172E-4
20	0.0	0.0	0.0	2.592390642161086E-4	6.480976605402715E-4
21	0.0	0.0	0.0	4.3206510702684765E-4	7.777171926483258E-4
22	0.0	0.0	0.0	6.048911498375868E-4	7.777171926483258E-4
23	0.0	0.0	0.0	0.0010369562568644344	7.777171926483258E-4
24	0.0	0.0	0.0	0.0013826083424859125	7.777171926483258E-4
25	0.0	0.0	0.0	0.0015554343852966516	7.777171926483258E-4
26	0.0	0.0	0.0	0.0021171190244315533	7.777171926483258E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	265	0.0	22.340437	4
TCTAGCG	805	0.0	18.682701	28
CTAGCGG	805	0.0	18.682701	29
TAAACGC	675	0.0	17.303213	28
AAACGCT	710	0.0	17.126274	29
CAAGACG	1005	0.0	16.876236	4
AACGCTT	710	0.0	16.675583	30
GTAATAC	135	4.4383341E-10	16.592838	3
GTATCAA	3890	0.0	16.57644	1
GACGGAC	1065	0.0	16.226646	7
AAGACGG	1075	0.0	16.2249	5
TAGCGGC	930	0.0	16.171583	30
GCGCAAG	1060	0.0	15.849635	1
ACGCTTC	740	0.0	15.783337	31
ACCGTCG	715	0.0	15.665585	8
CGCAAGA	1055	0.0	15.621084	2
GTAAACG	755	0.0	15.469759	27
TACCGTC	740	0.0	15.352575	7
GCGAAAG	1135	0.0	15.224237	18
ACACGCT	1085	0.0	15.189822	9
>>END_MODULE