FastQCFastQC Report
Thu 2 Feb 2017
SRR4062971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831308
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT86270.30470015978480613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT53810.1900535017737385No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30150.10648788475150002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29970.10585213618581943No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG29630.10465127778397829No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29540.10433340350113798No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28370.10020103782421412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG507.2000845E-419.1997689
GTAATAC1850.016.4316523
ACCGTCG6100.015.9983938
TACCGTC6400.015.7484187
GTATTAC1807.2759576E-1215.1253551
ATACCGT6800.015.0565136
GTATCAA60650.014.9984611
TAATACT4850.014.8425594
CGTCGTA6900.014.37689110
GGTATAG1604.84215E-914.0131961
CTAGCGG6200.013.93482329
TAGCGGC6350.013.60565430
CCGTCGT7300.013.5888779
TCTAGCG6500.013.5378228
AACCGCG3800.013.472337
TAATACG850.001611605513.1739834
TAACGAA4150.013.10850613
CGCGGTC4050.013.03710910
GTATAGG2109.640644E-1112.9645891
CAAGACG9150.012.93744854
Produced by FastQC (version 0.11.5)