##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062971_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2831308
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223049911913503	32.0	32.0	32.0	32.0	32.0
2	30.800960192250365	32.0	32.0	32.0	32.0	32.0
3	30.848758241773766	32.0	32.0	32.0	32.0	32.0
4	30.9253334501227	32.0	32.0	32.0	32.0	32.0
5	30.82845207939228	32.0	32.0	32.0	32.0	32.0
6	34.48443899427402	36.0	36.0	36.0	32.0	36.0
7	34.416842674834385	36.0	36.0	36.0	32.0	36.0
8	34.39947473040729	36.0	36.0	36.0	32.0	36.0
9	34.549834210901814	36.0	36.0	36.0	32.0	36.0
10	34.241528650362305	36.0	36.0	36.0	32.0	36.0
11	34.54124807332865	36.0	36.0	36.0	32.0	36.0
12	34.34774033768138	36.0	36.0	36.0	32.0	36.0
13	34.439713023097454	36.0	36.0	36.0	32.0	36.0
14	34.335829235109706	36.0	36.0	36.0	32.0	36.0
15	34.26949134463647	36.0	36.0	36.0	32.0	36.0
16	34.29144868732049	36.0	36.0	36.0	32.0	36.0
17	34.214833921283024	36.0	36.0	36.0	32.0	36.0
18	34.240205940152045	36.0	36.0	36.0	32.0	36.0
19	34.2320288008228	36.0	36.0	36.0	32.0	36.0
20	34.21039568990728	36.0	36.0	36.0	32.0	36.0
21	34.19929446036955	36.0	36.0	36.0	32.0	36.0
22	34.174518279184035	36.0	36.0	36.0	32.0	36.0
23	34.125277433610194	36.0	36.0	36.0	32.0	36.0
24	34.10680046112962	36.0	36.0	36.0	32.0	36.0
25	34.08357727241261	36.0	36.0	36.0	32.0	36.0
26	34.04151438133894	36.0	36.0	36.0	32.0	36.0
27	34.04240089739442	36.0	36.0	36.0	32.0	36.0
28	34.02821840647503	36.0	36.0	36.0	32.0	36.0
29	33.99944972429704	36.0	36.0	36.0	32.0	36.0
30	33.97292170261942	36.0	36.0	36.0	32.0	36.0
31	33.986424295767186	36.0	36.0	36.0	32.0	36.0
32	33.941128270043386	36.0	36.0	36.0	32.0	36.0
33	33.90592228044423	36.0	36.0	36.0	32.0	36.0
34	33.908469159837075	36.0	36.0	36.0	32.0	36.0
35	33.87070075032459	36.0	36.0	36.0	32.0	36.0
36	33.83538880263115	36.0	36.0	36.0	32.0	36.0
37	33.83202675229964	36.0	36.0	36.0	32.0	36.0
38	33.238531095875125	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	5.0
8	4.0
9	6.0
10	18.0
11	37.0
12	16.0
13	19.0
14	368.0
15	753.0
16	955.0
17	1177.0
18	1471.0
19	1878.0
20	2615.0
21	3850.0
22	5768.0
23	8736.0
24	13336.0
25	19812.0
26	28927.0
27	41027.0
28	56886.0
29	76092.0
30	101558.0
31	136982.0
32	185841.0
33	277898.0
34	613521.0
35	1251750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.68233157419024	17.132054548052047	11.303967761145051	25.881646116612657
2	16.822787256997685	19.94227772914777	37.394341634281666	25.840593379572873
3	18.890519663789558	24.39719318324317	28.350580102544537	28.361707050422737
4	12.809052737465526	15.538076710068266	35.95473631267237	35.698134239793845
5	14.795621935812456	36.33020484957954	33.41327313948342	15.46090007512458
6	33.68363165908534	35.54418975587548	17.153916167867706	13.61826241717147
7	29.991978969430022	30.48584982130161	21.19438125219201	18.32778995707636
8	28.11528273231378	32.41503902800834	19.721506021968708	19.74817221770918
9	27.893174235740304	14.24789725716296	18.44829693194156	39.41063157515517
10	15.924492609222835	26.357795485393808	31.549511372695004	26.168200532688353
11	37.24989068479003	21.318408540078593	22.464469907765512	18.96723086736586
12	25.328963982880982	23.679684886853344	28.431742026541755	22.559609103723922
13	29.56657631781507	19.52665585494823	25.106442203993883	25.800325623242813
14	24.04047019062374	19.631567681211944	25.217960157144663	31.110001971019653
15	25.21491712433928	26.66244145288004	22.617541137543427	25.50510028523726
16	25.83868400499761	25.71275468013775	23.48742631898163	24.96113499588301
17	24.26332605126089	25.60299657752016	25.031052433747742	25.10262493747121
18	25.041723490086497	24.67144848769347	26.043687717204982	24.24314030501505
19	25.760904081244107	24.731930261070627	25.547875310880364	23.959290346804902
20	25.952562354143772	24.116438436000927	24.9648638157172	24.966135394138096
21	27.199768855300384	24.05074021758826	24.543406212437663	24.206084714673697
22	26.163834134848884	24.01738549925029	24.901071846758942	24.917708519141886
23	24.577598195416268	23.932175514559628	25.595573033398296	25.89465325662581
24	24.94558210020241	24.85479728846609	25.20090996760713	24.99871064372437
25	24.989985198582737	24.323497514845574	25.309964285840447	25.37655300073124
26	24.880342209446795	25.137197119805453	25.629004857670022	24.353455813077733
27	25.539968456877233	24.59604139107644	24.7655547686455	25.09843538340083
28	24.6796595986972	24.559636809202264	25.703350147591298	25.05735344450924
29	24.69369935168747	24.82393408504713	25.53469711858743	24.947669444677967
30	24.54478987856825	24.962101143676577	25.75402123466209	24.739087743093084
31	25.127307599249065	24.853989654325915	24.67148334501887	25.34721940140615
32	24.91798217790167	24.826568687983293	24.5651148037577	25.690334330357334
33	24.557884883700392	24.405750516592782	25.25896752088448	25.777397078822347
34	25.333847651586122	24.603098908769265	25.30505854609012	24.757994893554493
35	25.83594389171729	24.377811030898556	25.334744942440544	24.45150013494361
36	24.67117836026149	25.12277161829631	24.959182101872713	25.24686791956949
37	25.567631702670806	24.928956625834463	24.884838204786476	24.618573466708256
38	24.648259195170272	24.80921948915858	25.376766121549217	25.16575519412193
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1181.0
1	901.5
2	622.0
3	622.0
4	1293.0
5	1964.0
6	1964.0
7	2331.5
8	2699.0
9	2528.5
10	2358.0
11	2358.0
12	3290.5
13	4223.0
14	5454.5
15	6686.0
16	6686.0
17	10121.5
18	13557.0
19	13557.0
20	16535.0
21	19513.0
22	20258.0
23	21003.0
24	21003.0
25	24454.0
26	27905.0
27	27905.0
28	35111.0
29	42317.0
30	52081.0
31	61845.0
32	61845.0
33	77634.5
34	93424.0
35	93424.0
36	104363.0
37	115302.0
38	132129.0
39	148956.0
40	148956.0
41	161768.5
42	174581.0
43	196694.5
44	218808.0
45	218808.0
46	225668.0
47	232528.0
48	232528.0
49	249040.0
50	265552.0
51	267690.5
52	269829.0
53	269829.0
54	259022.5
55	248216.0
56	248216.0
57	239709.5
58	231203.0
59	207810.0
60	184417.0
61	184417.0
62	172166.5
63	159916.0
64	132652.5
65	105389.0
66	105389.0
67	87735.5
68	70082.0
69	70082.0
70	56235.5
71	42389.0
72	33068.5
73	23748.0
74	23748.0
75	17960.5
76	12173.0
77	12173.0
78	11378.5
79	10584.0
80	8301.0
81	6018.0
82	6018.0
83	5947.0
84	5876.0
85	5876.0
86	3929.5
87	1983.0
88	1725.5
89	1468.0
90	1468.0
91	1075.0
92	682.0
93	612.5
94	543.0
95	543.0
96	489.5
97	436.0
98	436.0
99	884.0
100	1332.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12065095002027332
2	0.05478033474281145
3	0.012114542112691378
4	0.002684271721762521
5	3.8851301236036485E-4
6	2.472355533202322E-4
7	3.531936476003317E-5
8	2.8255491808026537E-4
9	2.11916188560199E-4
10	3.1787428284029854E-4
11	0.0016246907789615257
12	0.002507674897962355
13	0.01515200748205423
14	0.010101338321369486
15	0.02408780676634262
16	0.012538374489811776
17	0.0216154512331403
18	0.005968972644445606
19	0.008300050718607795
20	0.006392805021566004
21	0.006392805021566004
22	0.007523024693887066
23	0.010419212604209785
24	0.015469881764894527
25	0.01758904365049652
26	0.01861330522853748
27	0.009288992931888724
28	0.006322166292045938
29	0.009394951026168824
30	0.0031081040988829188
31	0.0061102501034857384
32	0.0076289827881671655
33	0.009112396108088559
34	0.013280081149772473
35	0.017518404920976453
36	0.015611159223934664
37	0.010277935145169654
38	0.006180888833005805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2831308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.09759287782421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50922879803036	37.828306028422055
2	13.827961420204215	13.854951631193016
3	4.5804719426076845	6.884118557071692
4	2.109829298656738	4.227894769832426
5	1.0910728112916495	2.7330060750076095
6	0.6507233495696145	1.9559804065699584
7	0.42332393288387166	1.4845257031538912
8	0.3184790091273304	1.276402539151509
9	0.22478993269260825	1.0135291077961017
>10	1.1103817163498282	9.70127209608828
>50	0.07053877447909629	2.4553154780002444
>100	0.06511913271018997	6.953784838368751
>500	0.01148604229931988	3.943672672314988
>1k	0.006452036106379401	5.189676740426212
>5k	1.4180299134899786E-4	0.4975633566033591
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8627	0.30470015978480613	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5381	0.1900535017737385	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3015	0.10648788475150002	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2997	0.10585213618581943	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2963	0.10465127778397829	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2954	0.10433340350113798	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2837	0.10020103782421412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.531936476003317E-5	0.0
3	0.0	0.0	0.0	3.531936476003317E-5	0.0
4	0.0	0.0	0.0	3.531936476003317E-5	0.0
5	0.0	0.0	0.0	3.531936476003317E-5	3.531936476003317E-5
6	3.531936476003317E-5	0.0	0.0	3.531936476003317E-5	3.531936476003317E-5
7	3.531936476003317E-5	0.0	0.0	3.531936476003317E-5	3.531936476003317E-5
8	3.531936476003317E-5	0.0	0.0	3.531936476003317E-5	3.531936476003317E-5
9	3.531936476003317E-5	0.0	0.0	3.531936476003317E-5	3.531936476003317E-5
10	3.531936476003317E-5	0.0	0.0	3.531936476003317E-5	3.531936476003317E-5
11	3.531936476003317E-5	0.0	0.0	1.0595809428009952E-4	3.531936476003317E-5
12	3.531936476003317E-5	0.0	0.0	1.0595809428009952E-4	1.4127745904013268E-4
13	3.531936476003317E-5	0.0	0.0	1.0595809428009952E-4	2.1191618856019904E-4
14	3.531936476003317E-5	0.0	0.0	1.0595809428009952E-4	2.1191618856019904E-4
15	3.531936476003317E-5	0.0	0.0	1.4127745904013268E-4	2.472355533202322E-4
16	1.0595809428009952E-4	0.0	0.0	2.1191618856019904E-4	2.472355533202322E-4
17	1.0595809428009952E-4	0.0	0.0	2.8255491808026537E-4	2.472355533202322E-4
18	1.0595809428009952E-4	0.0	0.0	2.8255491808026537E-4	2.8255491808026537E-4
19	1.0595809428009952E-4	0.0	0.0	3.1787428284029854E-4	3.1787428284029854E-4
20	1.0595809428009952E-4	0.0	0.0	3.885130123603649E-4	3.1787428284029854E-4
21	1.0595809428009952E-4	0.0	0.0	4.944711066404644E-4	3.531936476003317E-4
22	1.0595809428009952E-4	0.0	0.0	5.651098361605307E-4	3.531936476003317E-4
23	1.0595809428009952E-4	0.0	0.0	0.001236177766601161	3.531936476003317E-4
24	1.0595809428009952E-4	0.0	0.0	0.0019778844265618575	3.531936476003317E-4
25	1.0595809428009952E-4	3.531936476003317E-5	0.0	0.0024017168036822556	3.531936476003317E-4
26	1.0595809428009952E-4	3.531936476003317E-5	0.0	0.0031434234636429524	3.885130123603649E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACG	50	7.2000845E-4	19.199768	9
GTAATAC	185	0.0	16.431652	3
ACCGTCG	610	0.0	15.998393	8
TACCGTC	640	0.0	15.748418	7
GTATTAC	180	7.2759576E-12	15.125355	1
ATACCGT	680	0.0	15.056513	6
GTATCAA	6065	0.0	14.998461	1
TAATACT	485	0.0	14.842559	4
CGTCGTA	690	0.0	14.376891	10
GGTATAG	160	4.84215E-9	14.013196	1
CTAGCGG	620	0.0	13.934823	29
TAGCGGC	635	0.0	13.605654	30
CCGTCGT	730	0.0	13.588877	9
TCTAGCG	650	0.0	13.53782	28
AACCGCG	380	0.0	13.47233	7
TAATACG	85	0.0016116055	13.173983	4
TAACGAA	415	0.0	13.108506	13
CGCGGTC	405	0.0	13.037109	10
GTATAGG	210	9.640644E-11	12.964589	1
CAAGACG	915	0.0	12.9374485	4
>>END_MODULE