FastQCFastQC Report
Thu 2 Feb 2017
SRR4062971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831308
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT97490.3443284870455634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT63980.22597329573469221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT37730.13325996323960515No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32640.11528240657674828No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31140.1099845018627433No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC30730.10853640790758194No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28410.10034231528325424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA450.00885571817.77696415
TACCGTC7050.015.8888847
ATACCGT7450.015.0352576
ACCGTCG7150.014.9952338
CATCGTT6700.014.80529228
ACGAACG3200.014.49933615
TCGTTTA7100.014.42187530
CGTTTAT7000.014.3993431
GTATCAA61050.014.3890721
ATCGTTT7050.014.29721829
CGAACGA3250.013.78398416
TATACCG700.006801931713.71583755
CGTCGTA7750.013.62590410
TAACGAA3350.013.37299413
GCATCGT7550.013.35038227
CTTTCGT8750.013.3479627
AACCGCG4200.013.3353147
TTTCGTT9150.013.28900828
CGACTTT9300.013.24670524
TACGCTA7450.013.1019229
Produced by FastQC (version 0.11.5)