##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2831308
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.186136231028204	32.0	32.0	32.0	32.0	32.0
2	31.289028604447132	32.0	32.0	32.0	32.0	32.0
3	31.36895456093085	32.0	32.0	32.0	32.0	32.0
4	31.47277654003026	32.0	32.0	32.0	32.0	32.0
5	31.396836727053362	32.0	32.0	32.0	32.0	32.0
6	34.91712487655882	36.0	36.0	36.0	36.0	36.0
7	34.94177743996767	36.0	36.0	36.0	36.0	36.0
8	34.88512447250529	36.0	36.0	36.0	32.0	36.0
9	34.98793773054715	36.0	36.0	36.0	36.0	36.0
10	34.84054295753058	36.0	36.0	36.0	32.0	36.0
11	35.01554158007536	36.0	36.0	36.0	36.0	36.0
12	34.914608725013316	36.0	36.0	36.0	32.0	36.0
13	34.967607904191276	36.0	36.0	36.0	36.0	36.0
14	34.911828384619405	36.0	36.0	36.0	32.0	36.0
15	34.87997031760585	36.0	36.0	36.0	32.0	36.0
16	34.88739692043395	36.0	36.0	36.0	32.0	36.0
17	34.86171938905976	36.0	36.0	36.0	32.0	36.0
18	34.8563557903273	36.0	36.0	36.0	32.0	36.0
19	34.85095722542373	36.0	36.0	36.0	32.0	36.0
20	34.83660873348996	36.0	36.0	36.0	32.0	36.0
21	34.83315061448631	36.0	36.0	36.0	32.0	36.0
22	34.82115086030909	36.0	36.0	36.0	32.0	36.0
23	34.760889666542816	36.0	36.0	36.0	32.0	36.0
24	34.73596514402531	36.0	36.0	36.0	32.0	36.0
25	34.72678069641311	36.0	36.0	36.0	32.0	36.0
26	34.65893149032179	36.0	36.0	36.0	32.0	36.0
27	34.63949029918327	36.0	36.0	36.0	32.0	36.0
28	34.608400781546905	36.0	36.0	36.0	32.0	36.0
29	34.57956040105845	36.0	36.0	36.0	32.0	36.0
30	34.5574974534738	36.0	36.0	36.0	32.0	36.0
31	34.550936528275976	36.0	36.0	36.0	32.0	36.0
32	34.52332702764941	36.0	36.0	36.0	32.0	36.0
33	34.486507296274375	36.0	36.0	36.0	32.0	36.0
34	34.479024535656315	36.0	36.0	36.0	32.0	36.0
35	34.4468817945628	36.0	36.0	36.0	32.0	36.0
36	34.426891034108614	36.0	36.0	36.0	32.0	36.0
37	34.4157520128506	36.0	36.0	36.0	32.0	36.0
38	33.99370397003788	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	21.0
21	72.0
22	282.0
23	737.0
24	2031.0
25	4848.0
26	10247.0
27	19439.0
28	33593.0
29	52406.0
30	78875.0
31	112919.0
32	160954.0
33	257266.0
34	628850.0
35	1468760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.029279108236025	18.322940888281185	11.948692063723062	26.699087939759732
2	15.793336507366911	20.98436482360803	36.66997726845684	26.552321400568218
3	17.84288392502688	25.152544336398584	28.668163265882768	28.336408472691772
4	12.281752661139418	16.4341826704708	35.99822837941218	35.2858362889776
5	14.311063516271288	36.8473771468008	33.10617079185365	15.735388545074253
6	33.75783782791021	35.964985707983715	16.782991282099644	13.494185182006435
7	29.748432870929598	30.723252847110082	21.130459168464434	18.397855113495886
8	27.978337437536293	32.61029427347131	19.56482733385042	19.846540955141982
9	27.760119327872967	14.092585064024293	18.3113396446937	39.835955963409035
10	15.748787390628516	26.599667784060344	31.541123819487293	26.11042100582385
11	37.27817924102783	21.27172820009812	22.396068623195138	19.054023935678913
12	25.18779359304913	23.82824144385971	28.397061420548862	22.586903542542295
13	29.526072996124398	19.510571288605487	25.136606810791225	25.826748904478897
14	23.904675860061854	19.605602781470612	25.238829544507347	31.25089181396019
15	25.209832346039356	26.75759754855353	22.437191573647233	25.595378531759877
16	25.807093059273416	25.713955729923644	23.47696449302671	25.00198671777623
17	24.274504928464157	25.62780170860959	24.980291794463902	25.11740156846235
18	25.059018658514017	24.645111022891186	26.03199651892341	24.26387379967139
19	25.72538911344156	24.752693807950248	25.53907946433239	23.982837614275805
20	25.909191087652772	24.047507371151426	24.971426633909132	25.071874907286666
21	27.06048977415008	24.125730007893885	24.590780576447962	24.222999641508068
22	26.034301472782712	24.000152579709656	25.06640925904538	24.899136688462253
23	24.532812112589898	23.869972189473255	25.618355088930816	25.978860609006034
24	24.930950641894135	24.752623169220726	25.280117881911824	25.036308306973314
25	24.962420195895323	24.22269142036119	25.45106360735038	25.363824776393102
26	24.915092127161195	24.967541457928927	25.649841910299987	24.467524504609887
27	25.488581775130708	24.552300404018357	24.73853948017647	25.220578340674464
28	24.654258738364035	24.473176355239346	25.822340769707854	25.050224136688765
29	24.772366694121587	24.64814848826055	25.599581536166323	24.97990328145154
30	24.60463503087619	24.765514737358142	25.849218806290235	24.780631425475434
31	25.17009806068432	24.642356112439902	24.78412804258668	25.4034177842891
32	24.966941074584607	24.64747742033011	24.70995737659061	25.675624128494672
33	24.586975348496175	24.219371400073747	25.421077466669118	25.772575784760964
34	25.226573724935612	24.41373386434821	25.52046615910385	24.839226251612327
35	25.86359378774757	24.209658574764738	25.380142322912235	24.546605314575455
36	24.674743969924855	24.991982504199473	25.14516258916374	25.188110936711936
37	25.578319278580786	24.773496913793906	24.971532592003413	24.6766512156219
38	24.760004916455575	24.56666671375915	25.478330156945127	25.19499821284014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	629.0
1	493.0
2	357.0
3	357.0
4	1149.0
5	1941.0
6	1941.0
7	2274.0
8	2607.0
9	2577.0
10	2547.0
11	2547.0
12	3444.0
13	4341.0
14	5613.0
15	6885.0
16	6885.0
17	10600.0
18	14315.0
19	14315.0
20	17206.5
21	20098.0
22	20644.0
23	21190.0
24	21190.0
25	24412.0
26	27634.0
27	27634.0
28	35106.5
29	42579.0
30	52021.5
31	61464.0
32	61464.0
33	77741.0
34	94018.0
35	94018.0
36	104720.5
37	115423.0
38	132000.0
39	148577.0
40	148577.0
41	161919.5
42	175262.0
43	197617.5
44	219973.0
45	219973.0
46	225999.0
47	232025.0
48	232025.0
49	249293.0
50	266561.0
51	268359.0
52	270157.0
53	270157.0
54	259155.5
55	248154.0
56	248154.0
57	240640.5
58	233127.0
59	208967.0
60	184807.0
61	184807.0
62	173425.0
63	162043.0
64	133533.5
65	105024.0
66	105024.0
67	87469.0
68	69914.0
69	69914.0
70	55933.0
71	41952.0
72	32472.0
73	22992.0
74	22992.0
75	17199.0
76	11406.0
77	11406.0
78	10817.0
79	10228.0
80	7887.5
81	5547.0
82	5547.0
83	5268.5
84	4990.0
85	4990.0
86	3164.0
87	1338.0
88	1118.5
89	899.0
90	899.0
91	542.0
92	185.0
93	120.5
94	56.0
95	56.0
96	45.0
97	34.0
98	34.0
99	31.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008653244366208127
2	0.0
3	0.0
4	7.063872952006634E-5
5	7.063872952006634E-5
6	2.472355533202322E-4
7	5.651098361605307E-4
8	0.002260439344642123
9	0.004132365676923881
10	6.004292009205639E-4
11	0.004838752972124545
12	2.8255491808026537E-4
13	2.472355533202322E-4
14	0.0
15	0.0
16	1.7659682380016586E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.059580942800995E-4
22	3.531936476003317E-5
23	2.11916188560199E-4
24	0.0
25	0.0
26	1.4127745904013268E-4
27	3.531936476003317E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2831308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.07816402501072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46426418592297	35.320269435075254
2	15.008422906597419	14.431548365202376
3	5.065938351984141	7.306830450818583
4	2.211749629279128	4.253474458349543
5	1.1529527001327189	2.771592451502993
6	0.7015965457354114	2.0238884283148826
7	0.4725275855099702	1.5902781133743438
8	0.31852375410961725	1.2251229836755488
9	0.24611473807507286	1.0649470271531312
>10	1.194123658331418	10.066035867043869
>50	0.07486975562443832	2.5164982884835316
>100	0.06883465246018669	7.049183322329414
>500	0.012624789325864092	4.0885736998135185
>1k	0.007309088557048264	5.718607831347061
>5k	1.4765835468784374E-4	0.5731492775159428
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9749	0.3443284870455634	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6398	0.22597329573469221	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3773	0.13325996323960515	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3264	0.11528240657674828	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3114	0.1099845018627433	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3073	0.10853640790758194	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2841	0.10034231528325424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7659682380016586E-4
2	0.0	0.0	0.0	0.0	1.7659682380016586E-4
3	0.0	0.0	0.0	0.0	1.7659682380016586E-4
4	0.0	0.0	0.0	0.0	1.7659682380016586E-4
5	0.0	0.0	0.0	0.0	2.1191618856019904E-4
6	7.063872952006634E-5	0.0	0.0	0.0	2.1191618856019904E-4
7	7.063872952006634E-5	0.0	0.0	0.0	2.1191618856019904E-4
8	7.063872952006634E-5	0.0	0.0	0.0	2.1191618856019904E-4
9	7.063872952006634E-5	0.0	0.0	0.0	2.1191618856019904E-4
10	7.063872952006634E-5	3.531936476003317E-5	0.0	0.0	2.1191618856019904E-4
11	7.063872952006634E-5	3.531936476003317E-5	0.0	3.531936476003317E-5	2.1191618856019904E-4
12	7.063872952006634E-5	3.531936476003317E-5	0.0	3.531936476003317E-5	2.8255491808026537E-4
13	7.063872952006634E-5	3.531936476003317E-5	0.0	3.531936476003317E-5	4.238323771203981E-4
14	7.063872952006634E-5	3.531936476003317E-5	0.0	3.531936476003317E-5	4.238323771203981E-4
15	7.063872952006634E-5	3.531936476003317E-5	0.0	7.063872952006634E-5	4.238323771203981E-4
16	1.7659682380016586E-4	3.531936476003317E-5	0.0	1.4127745904013268E-4	4.238323771203981E-4
17	1.7659682380016586E-4	3.531936476003317E-5	0.0	2.1191618856019904E-4	4.238323771203981E-4
18	1.7659682380016586E-4	3.531936476003317E-5	0.0	2.1191618856019904E-4	4.5915174188043126E-4
19	1.7659682380016586E-4	3.531936476003317E-5	0.0	2.472355533202322E-4	4.5915174188043126E-4
20	1.7659682380016586E-4	3.531936476003317E-5	0.0	3.1787428284029854E-4	4.944711066404644E-4
21	1.7659682380016586E-4	3.531936476003317E-5	0.0	4.238323771203981E-4	5.297904714004976E-4
22	1.7659682380016586E-4	3.531936476003317E-5	0.0	4.944711066404644E-4	5.297904714004976E-4
23	1.7659682380016586E-4	3.531936476003317E-5	0.0	0.0010949003075610283	5.651098361605307E-4
24	1.7659682380016586E-4	3.531936476003317E-5	0.0	0.0018719263322817582	5.651098361605307E-4
25	1.7659682380016586E-4	3.531936476003317E-5	0.0	0.002260439344642123	5.651098361605307E-4
26	1.7659682380016586E-4	3.531936476003317E-5	0.0	0.0029668266398427865	5.651098361605307E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	45	0.008855718	17.776964	15
TACCGTC	705	0.0	15.888884	7
ATACCGT	745	0.0	15.035257	6
ACCGTCG	715	0.0	14.995233	8
CATCGTT	670	0.0	14.805292	28
ACGAACG	320	0.0	14.499336	15
TCGTTTA	710	0.0	14.421875	30
CGTTTAT	700	0.0	14.39934	31
GTATCAA	6105	0.0	14.389072	1
ATCGTTT	705	0.0	14.297218	29
CGAACGA	325	0.0	13.783984	16
TATACCG	70	0.0068019317	13.7158375	5
CGTCGTA	775	0.0	13.625904	10
TAACGAA	335	0.0	13.372994	13
GCATCGT	755	0.0	13.350382	27
CTTTCGT	875	0.0	13.34796	27
AACCGCG	420	0.0	13.335314	7
TTTCGTT	915	0.0	13.289008	28
CGACTTT	930	0.0	13.246705	24
TACGCTA	745	0.0	13.101922	9
>>END_MODULE