FastQCFastQC Report
Thu 2 Feb 2017
SRR4062970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887103
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62770.3326262530450113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40880.2166283451406733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22320.11827653286545568No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19950.10571759994022585No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19270.10211419302497002No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19020.10078941107083185No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18990.10063043723633527No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18990.10063043723633527No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18920.10025949828917659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2650.018.11409216
ACGAACG2650.018.11409215
CGATAAC2650.018.11361310
ATACGAC450.00886064417.7748783
TAACGAA2750.017.45539913
CCGATAA2750.016.8726589
AACGAAC2900.016.55253214
ATAACGA2900.016.55209412
TCTAGCG4850.016.49346528
TACCGTC3400.016.469217
CTAGCGG4800.016.332429
CGCCAAA600.002441888716.00078214
GATAACG3000.016.00035911
CGTCGTA3500.016.00035710
ATCGTTT4000.015.99908629
ACCGTCG3550.015.7733298
GTAGGAC8700.015.6296333
GTCCTAC7850.015.5051751
TAGCGGC4850.015.50467930
CATCGTT4150.015.42039728
Produced by FastQC (version 0.11.5)