##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062970_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1887103
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188105259755297	32.0	32.0	32.0	32.0	32.0
2	30.810681239974713	32.0	32.0	32.0	32.0	32.0
3	30.87108122874056	32.0	32.0	32.0	32.0	32.0
4	30.931767900321287	32.0	32.0	32.0	32.0	32.0
5	30.853577679649707	32.0	32.0	32.0	32.0	32.0
6	34.50518546152489	36.0	36.0	36.0	32.0	36.0
7	34.415072733178846	36.0	36.0	36.0	32.0	36.0
8	34.409791092484085	36.0	36.0	36.0	32.0	36.0
9	34.53001982403716	36.0	36.0	36.0	32.0	36.0
10	34.255998215253754	36.0	36.0	36.0	32.0	36.0
11	34.53202554391573	36.0	36.0	36.0	32.0	36.0
12	34.35388158462999	36.0	36.0	36.0	32.0	36.0
13	34.431226594414824	36.0	36.0	36.0	32.0	36.0
14	34.33718721235672	36.0	36.0	36.0	32.0	36.0
15	34.28863183408643	36.0	36.0	36.0	32.0	36.0
16	34.292295651058794	36.0	36.0	36.0	32.0	36.0
17	34.23341492223795	36.0	36.0	36.0	32.0	36.0
18	34.26060209750077	36.0	36.0	36.0	32.0	36.0
19	34.23761819042204	36.0	36.0	36.0	32.0	36.0
20	34.21526964876851	36.0	36.0	36.0	32.0	36.0
21	34.18931240107191	36.0	36.0	36.0	32.0	36.0
22	34.170673778802744	36.0	36.0	36.0	32.0	36.0
23	34.11678482838509	36.0	36.0	36.0	32.0	36.0
24	34.10510873015411	36.0	36.0	36.0	32.0	36.0
25	34.086249664167774	36.0	36.0	36.0	32.0	36.0
26	34.04402356416158	36.0	36.0	36.0	32.0	36.0
27	34.0392940925853	36.0	36.0	36.0	32.0	36.0
28	34.022089944216084	36.0	36.0	36.0	32.0	36.0
29	33.98524722815872	36.0	36.0	36.0	32.0	36.0
30	33.95773256679683	36.0	36.0	36.0	32.0	36.0
31	33.97864398498651	36.0	36.0	36.0	32.0	36.0
32	33.93212718118725	36.0	36.0	36.0	32.0	36.0
33	33.88196033814794	36.0	36.0	36.0	32.0	36.0
34	33.896598118915605	36.0	36.0	36.0	32.0	36.0
35	33.85608840640919	36.0	36.0	36.0	32.0	36.0
36	33.80956312400542	36.0	36.0	36.0	32.0	36.0
37	33.805693700873775	36.0	36.0	36.0	32.0	36.0
38	33.199968417198214	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	3.0
8	3.0
9	6.0
10	8.0
11	21.0
12	10.0
13	8.0
14	382.0
15	655.0
16	775.0
17	944.0
18	1163.0
19	1411.0
20	1940.0
21	2779.0
22	4010.0
23	6051.0
24	8784.0
25	13296.0
26	19139.0
27	27203.0
28	38148.0
29	50427.0
30	67840.0
31	90312.0
32	122704.0
33	181766.0
34	401343.0
35	845970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.595581123532554	17.331441732194484	11.480653621056195	25.59232352321677
2	16.234925465723247	19.969884069574164	38.55793472584164	25.23725573886095
3	18.982005081441926	24.59597024907969	28.396293112494476	28.025731556983907
4	12.38323056280378	15.596390560514495	36.61775086219139	35.402628014490325
5	14.259407831495768	36.95282385970416	33.768940457782264	15.018827851017807
6	33.38696070576166	36.04013786262066	17.284176321713364	13.288725109904318
7	29.773679550082853	30.735047318561836	21.172612199758042	18.318660931597268
8	27.64930316358434	33.3837104551958	19.76779184992846	19.1991945312914
9	27.731999080068974	14.464219664267569	18.688854526897913	39.11492672876554
10	15.64521129036959	26.838288480442163	31.95684372010948	25.55965650907877
11	36.97871623340286	21.459594546471994	22.790121415611065	18.771567804514085
12	25.179115360890957	23.76420999527305	29.106458115621614	21.950216528214376
13	29.53506305599648	19.956487519974136	25.307780572022164	25.20066885200722
14	23.56858228979137	20.24470526884245	25.541847759553516	30.644864681812667
15	24.762726076756643	27.815404969050768	22.866336838333588	24.555532115859005
16	25.032553756759178	26.079891715367015	24.655794038727443	24.23176048914636
17	23.545487166195738	26.10970388991979	25.843312413174985	24.50149653070949
18	24.299521567613287	25.038844809421963	27.244625849763327	23.417007773201426
19	25.266723618580457	25.137574908371718	26.065887570138823	23.529813902908998
20	25.525770923125755	24.58496092456191	25.784807664689446	24.104460487622887
21	26.59755833234852	24.41233096748312	24.920230059634687	24.069880640533675
22	25.465391176606193	24.875447876241218	25.447478776720878	24.211682170431715
23	23.775979547590065	24.6853995167779	26.186949399470137	25.351671536161895
24	24.45764595581669	25.44480902003953	25.89053227624666	24.207012747897124
25	24.471052023464516	24.930091945786543	25.997475041923636	24.601380988825312
26	23.82468485123452	25.909617072519197	26.481665613697363	23.78403246254892
27	24.6943572214052	25.38844946142533	25.60186546895081	24.31532784821866
28	23.885852918170812	25.36634792507064	26.160145924438954	24.587653232319592
29	23.959164724325323	25.477688778089753	26.094150293232598	24.46899620435233
30	23.966283900117165	25.533597131185115	26.393722268237585	24.106396700460138
31	24.66221587235639	25.352463481794423	25.257816093659578	24.727504552189608
32	24.015955628641063	25.64829677850834	25.33584033348472	24.999907259365877
33	23.673407739514772	25.27494249949654	26.166597772054224	24.885051988934464
34	24.52793390094151	25.41458980902184	26.14516671833968	23.91230957169697
35	25.00715501688584	25.353192833535086	26.098003591288478	23.5416485582906
36	23.8413260038626	25.929504370334723	25.724820490859713	24.504349134942963
37	24.73228717850878	25.779554933727628	25.398402679513065	24.089755208250526
38	23.97340310240611	25.78263655234509	25.79620315349284	24.447757191755958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1240.0
1	904.5
2	569.0
3	569.0
4	1184.0
5	1799.0
6	1799.0
7	2104.5
8	2410.0
9	2222.5
10	2035.0
11	2035.0
12	2650.5
13	3266.0
14	4188.0
15	5110.0
16	5110.0
17	7658.0
18	10206.0
19	10206.0
20	12267.5
21	14329.0
22	15177.0
23	16025.0
24	16025.0
25	19262.0
26	22499.0
27	22499.0
28	29170.5
29	35842.0
30	43800.0
31	51758.0
32	51758.0
33	63669.0
34	75580.0
35	75580.0
36	83936.0
37	92292.0
38	101749.0
39	111206.0
40	111206.0
41	117106.5
42	123007.0
43	135675.0
44	148343.0
45	148343.0
46	152554.5
47	156766.0
48	156766.0
49	164107.0
50	171448.0
51	170782.5
52	170117.0
53	170117.0
54	160967.0
55	151817.0
56	151817.0
57	146562.5
58	141308.0
59	126568.0
60	111828.0
61	111828.0
62	104209.0
63	96590.0
64	79929.0
65	63268.0
66	63268.0
67	51968.5
68	40669.0
69	40669.0
70	32824.5
71	24980.0
72	19577.0
73	14174.0
74	14174.0
75	10994.0
76	7814.0
77	7814.0
78	7435.0
79	7056.0
80	5459.0
81	3862.0
82	3862.0
83	3838.5
84	3815.0
85	3815.0
86	2561.0
87	1307.0
88	1103.5
89	900.0
90	900.0
91	678.0
92	456.0
93	388.5
94	321.0
95	321.0
96	295.0
97	269.0
98	269.0
99	545.5
100	822.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12082011421740096
2	0.05606477229912729
3	0.012558932925229837
4	0.0027025551864418634
5	2.6495639082763366E-4
6	3.7093894715868717E-4
7	0.0
8	1.589738344965802E-4
9	2.6495639082763366E-4
10	4.2393022532421387E-4
11	0.0016427296231313288
12	0.0027025551864418634
13	0.0152614881116717
14	0.009538430069794813
15	0.024640944346969933
16	0.01187004630907799
17	0.021514458935203852
18	0.00567006676371136
19	0.006941857439684002
20	0.005935023154538995
21	0.006041005710870048
22	0.0075247614995047965
23	0.009856377738787973
24	0.01494354044267854
25	0.017964043298113564
26	0.019871729312072525
27	0.00943244751346376
28	0.005246136538387147
29	0.010863212023932981
30	0.00275554646460739
31	0.005246136538387147
32	0.006358953379863207
33	0.008637578340980858
34	0.012611924203395361
35	0.01669225262214092
36	0.01542046194616828
37	0.01022731668594666
38	0.006146988267201101
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1887103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.674521848496106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56385852501029	41.09528497205829
2	13.980698342820322	15.008145973178852
3	4.4053728260445455	7.09368840006897
4	1.8730016773854492	4.021298778203806
5	0.9579648944396559	2.5709153828346785
6	0.5428903285410572	1.748362728036852
7	0.3315686716489049	1.2457752937487183
8	0.2271947491663955	0.975565562239623
9	0.17442228373111393	0.8425829411091234
>10	0.7938553618225562	7.578756862962593
>50	0.06625860206295599	2.503096637613466
>100	0.06820110077446698	7.886522245145308
>500	0.010537428882020722	3.7844471379061115
>1k	0.004075797963800468	3.3106308986623043
>5k	9.940970643415775E-5	0.3349261862312362
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6277	0.3326262530450113	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4088	0.2166283451406733	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2232	0.11827653286545568	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1995	0.10571759994022585	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1927	0.10211419302497002	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1902	0.10078941107083185	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1899	0.10063043723633527	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1899	0.10063043723633527	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1892	0.10025949828917659	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.299127816552673E-5	0.0
12	0.0	0.0	0.0	5.299127816552673E-5	0.0
13	0.0	0.0	0.0	1.589738344965802E-4	0.0
14	0.0	0.0	0.0	1.589738344965802E-4	0.0
15	0.0	0.0	0.0	2.1196511266210693E-4	0.0
16	0.0	0.0	0.0	2.6495639082763366E-4	0.0
17	0.0	0.0	0.0	3.179476689931604E-4	0.0
18	0.0	0.0	0.0	3.179476689931604E-4	0.0
19	0.0	0.0	0.0	4.2393022532421387E-4	0.0
20	0.0	5.299127816552673E-5	0.0	5.299127816552673E-4	0.0
21	0.0	5.299127816552673E-5	0.0	6.888866161518475E-4	0.0
22	0.0	5.299127816552673E-5	0.0	8.478604506484277E-4	0.0
23	0.0	5.299127816552673E-5	0.0	0.0019076860139589625	0.0
24	0.0	5.299127816552673E-5	0.0	0.0035504156370902915	0.0
25	0.0	5.299127816552673E-5	0.0	0.004504258644069773	0.0
26	0.0	1.0598255633105347E-4	0.0	0.005564084207380307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	265	0.0	18.114092	16
ACGAACG	265	0.0	18.114092	15
CGATAAC	265	0.0	18.113613	10
ATACGAC	45	0.008860644	17.774878	3
TAACGAA	275	0.0	17.455399	13
CCGATAA	275	0.0	16.872658	9
AACGAAC	290	0.0	16.552532	14
ATAACGA	290	0.0	16.552094	12
TCTAGCG	485	0.0	16.493465	28
TACCGTC	340	0.0	16.46921	7
CTAGCGG	480	0.0	16.3324	29
CGCCAAA	60	0.0024418887	16.000782	14
GATAACG	300	0.0	16.000359	11
CGTCGTA	350	0.0	16.000357	10
ATCGTTT	400	0.0	15.999086	29
ACCGTCG	355	0.0	15.773329	8
GTAGGAC	870	0.0	15.629633	3
GTCCTAC	785	0.0	15.505175	1
TAGCGGC	485	0.0	15.504679	30
CATCGTT	415	0.0	15.420397	28
>>END_MODULE