FastQCFastQC Report
Thu 2 Feb 2017
SRR4062970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887103
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT76130.40342260067415503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT51660.27375294300311115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29130.15436359329617938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23420.1241055734636636No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21620.1145671433938688No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20580.10905605046465403No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20530.10879109407382638No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19900.1054526435493982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5350.018.54144329
TCTAGCG5600.017.99940328
GTCCTAC7900.017.8245561
TAGCGGC5650.017.55694230
AACCGCG2800.017.1441067
CGAACGA1505.456968E-1217.06655316
ACGTTTT3950.017.01209629
TTATACC1151.843E-816.6955414
ACGAACG1551.0913936E-1116.5160215
GTAGGAC8250.016.0964373
GACGCGC703.6989021E-415.99946926
AGCGGCG6250.015.87147431
TAGGACG5550.015.5674644
CGCGGTC3100.015.48499910
GAACCGC3450.015.3054636
CGCCGGT6850.015.1835887
TAGCGTA3700.015.1362387
CGCGGTT6050.015.07547910
CGGTCCA6900.015.07356210
GTTTTCG5750.015.0255928
Produced by FastQC (version 0.11.5)