##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1887103
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.154961334913885	32.0	32.0	32.0	32.0	32.0
2	31.2744725645606	32.0	32.0	32.0	32.0	32.0
3	31.36751094137416	32.0	32.0	32.0	32.0	32.0
4	31.4690655465017	32.0	32.0	32.0	32.0	32.0
5	31.387085389615724	32.0	32.0	32.0	32.0	32.0
6	34.90402908585276	36.0	36.0	36.0	36.0	36.0
7	34.93558592191311	36.0	36.0	36.0	36.0	36.0
8	34.87188192695364	36.0	36.0	36.0	32.0	36.0
9	34.98717187138169	36.0	36.0	36.0	36.0	36.0
10	34.825732352712066	36.0	36.0	36.0	32.0	36.0
11	35.01214507104276	36.0	36.0	36.0	36.0	36.0
12	34.90650536828144	36.0	36.0	36.0	32.0	36.0
13	34.95694087710104	36.0	36.0	36.0	36.0	36.0
14	34.90392628277312	36.0	36.0	36.0	32.0	36.0
15	34.86667712361223	36.0	36.0	36.0	32.0	36.0
16	34.88219614933578	36.0	36.0	36.0	32.0	36.0
17	34.8521103511573	36.0	36.0	36.0	32.0	36.0
18	34.85219460728958	36.0	36.0	36.0	32.0	36.0
19	34.84239916952069	36.0	36.0	36.0	32.0	36.0
20	34.82801415714987	36.0	36.0	36.0	32.0	36.0
21	34.8177068236339	36.0	36.0	36.0	32.0	36.0
22	34.802114140033694	36.0	36.0	36.0	32.0	36.0
23	34.74189909082864	36.0	36.0	36.0	32.0	36.0
24	34.722240916367575	36.0	36.0	36.0	32.0	36.0
25	34.703704037352495	36.0	36.0	36.0	32.0	36.0
26	34.636338345071785	36.0	36.0	36.0	32.0	36.0
27	34.6196386736707	36.0	36.0	36.0	32.0	36.0
28	34.59090945221326	36.0	36.0	36.0	32.0	36.0
29	34.55446046135267	36.0	36.0	36.0	32.0	36.0
30	34.52986826898161	36.0	36.0	36.0	32.0	36.0
31	34.523345042639434	36.0	36.0	36.0	32.0	36.0
32	34.48285758646984	36.0	36.0	36.0	32.0	36.0
33	34.450928221723984	36.0	36.0	36.0	32.0	36.0
34	34.43695866097399	36.0	36.0	36.0	32.0	36.0
35	34.40965702455033	36.0	36.0	36.0	32.0	36.0
36	34.3808928288493	36.0	36.0	36.0	32.0	36.0
37	34.36108574889659	36.0	36.0	36.0	32.0	36.0
38	33.92902878115291	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	15.0
21	54.0
22	153.0
23	476.0
24	1330.0
25	3176.0
26	6656.0
27	12709.0
28	22359.0
29	35643.0
30	53526.0
31	76883.0
32	110444.0
33	176941.0
34	428118.0
35	958615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.9860296662245	18.546292463884402	12.17609372275663	26.29158414713447
2	15.314540496486146	21.05901005880975	37.670989697430244	25.955459747273864
3	17.996738916741695	25.310012225087874	28.777496511849115	27.91575234632132
4	11.847550128530617	16.561293286679714	36.59898076359534	34.99217582119433
5	13.719728684224473	37.44099411795877	33.46404536060622	15.375231837210535
6	33.3596170212315	36.45743579308379	16.96633250348948	13.216614682195239
7	29.54578935487187	30.924255126124876	21.187399912458176	18.342555606545076
8	27.577212783072913	33.612586364595046	19.491345787993797	19.318855064338244
9	27.57174342633451	14.25769606001366	18.574410570645938	39.596149943005884
10	15.417661806093935	27.125376704543346	31.925415282480053	25.531546206882666
11	37.10309500706674	21.30649223876753	22.772835500875196	18.81757725329053
12	25.05999975623857	23.831071209602506	29.072153913086336	22.036775121072594
13	29.605055375973716	19.92226167134757	25.314344761803824	25.158338190874886
14	23.455688428241594	20.21781534977158	25.601252289885608	30.725243932101215
15	24.761923435021828	27.762978491370106	22.87718264450854	24.597915429099523
16	25.03096015426821	26.09412416810317	24.56802834821417	24.30688732941445
17	23.449965370199717	26.20302124473333	25.758583394759054	24.588429990307894
18	24.28341219318712	24.946333083037864	27.327125228458648	23.443129495316366
19	25.205831372214448	25.06222500838587	26.201484497666527	23.530459121733156
20	25.525421770830736	24.453514196098464	25.942887060218762	24.078176972852038
21	26.48325630147454	24.411079869725896	25.068915339849863	24.036748488949698
22	25.301003707799737	24.82413519558816	25.707075872382163	24.16778522422994
23	23.65164731686043	24.573273580241416	26.37259624428819	25.40248285860996
24	24.416579275217092	25.265870490375992	26.07827977593168	24.23927045847524
25	24.469914434892463	24.786060735487926	26.159225181906002	24.58479964771361
26	23.88565965927668	25.677188791496807	26.606867775632807	23.830283773593706
27	24.70458687204673	25.150720442922296	25.675069140370187	24.469623544660784
28	23.871510988006484	25.199366436278254	26.39416078507638	24.534961790638878
29	24.016177177398372	25.168737477498578	26.28049449341133	24.53459085169172
30	23.960218387655576	25.31631818718957	26.629812999078485	24.093650426076373
31	24.58710520835376	25.092642002052884	25.540948215333238	24.779304574260124
32	24.149291268150176	25.456904048162716	25.513604715799826	24.880199967887286
33	23.670038148421153	24.939179260485517	26.401314607628727	24.989467983464603
34	24.52865582853718	25.120568405646114	26.35876261126181	23.992013154554893
35	25.03567637802494	25.094973618292165	26.190303337973603	23.67904666570929
36	23.940770588568828	25.649262387903576	25.9534323245737	24.4565346989539
37	24.817087355592143	25.476987742587447	25.564105403891574	24.141819497928836
38	24.11761526403978	25.399845901281438	25.99197075727756	24.49056807740122
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	651.0
1	482.5
2	314.0
3	314.0
4	991.5
5	1669.0
6	1669.0
7	1980.5
8	2292.0
9	2221.5
10	2151.0
11	2151.0
12	2756.0
13	3361.0
14	4437.0
15	5513.0
16	5513.0
17	8297.0
18	11081.0
19	11081.0
20	13171.5
21	15262.0
22	15829.5
23	16397.0
24	16397.0
25	19340.5
26	22284.0
27	22284.0
28	28920.0
29	35556.0
30	43454.0
31	51352.0
32	51352.0
33	63872.5
34	76393.0
35	76393.0
36	84212.0
37	92031.0
38	101699.5
39	111368.0
40	111368.0
41	117522.0
42	123676.0
43	136152.0
44	148628.0
45	148628.0
46	152870.0
47	157112.0
48	157112.0
49	165074.0
50	173036.0
51	170896.5
52	168757.0
53	168757.0
54	160662.0
55	152567.0
56	152567.0
57	146936.0
58	141305.0
59	126156.5
60	111008.0
61	111008.0
62	104157.0
63	97306.0
64	80031.5
65	62757.0
66	62757.0
67	51866.5
68	40976.0
69	40976.0
70	33096.0
71	25216.0
72	19630.5
73	14045.0
74	14045.0
75	10782.0
76	7519.0
77	7519.0
78	7173.5
79	6828.0
80	5251.5
81	3675.0
82	3675.0
83	3500.5
84	3326.0
85	3326.0
86	2118.5
87	911.0
88	751.0
89	591.0
90	591.0
91	350.0
92	109.0
93	74.5
94	40.0
95	40.0
96	30.5
97	21.0
98	21.0
99	20.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009326464957132706
2	5.299127816552673E-5
3	0.0
4	2.1196511266210693E-4
5	1.589738344965802E-4
6	5.299127816552673E-5
7	2.6495639082763366E-4
8	0.002384607517448703
9	0.0034974243589247643
10	7.418778943173743E-4
11	0.005087162703890567
12	6.358953379863208E-4
13	1.589738344965802E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.6495639082763366E-4
22	0.0
23	2.1196511266210693E-4
24	0.0
25	1.0598255633105347E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.299127816552673E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1887103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.40651258915141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79472158832402	38.449357969322534
2	14.885613496858777	15.304349552470256
3	4.788151780352202	7.384265543263297
4	2.0225996579408063	4.158991791149895
5	1.0580174391372856	2.71944934022763
6	0.5868538463686225	1.810086578480431
7	0.3634139518461491	1.307729072345663
8	0.2669563442153561	1.097863573572843
9	0.19256958342728508	0.8909397643268137
>10	0.8814805470575346	8.019316514297266
>50	0.06912648997867488	2.4883149999041083
>100	0.07534448506145226	8.48727422261007
>500	0.010688570626568307	3.8900852628832387
>1k	0.004254673744556316	3.3102693400852097
>5k	2.0754506071006423E-4	0.6817064750608173
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7613	0.40342260067415503	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5166	0.27375294300311115	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2913	0.15436359329617938	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2342	0.1241055734636636	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2162	0.1145671433938688	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2058	0.10905605046465403	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2053	0.10879109407382638	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1990	0.1054526435493982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.299127816552673E-5	0.0
12	0.0	0.0	0.0	5.299127816552673E-5	0.0
13	0.0	0.0	0.0	1.589738344965802E-4	0.0
14	0.0	0.0	0.0	1.589738344965802E-4	0.0
15	0.0	0.0	0.0	2.1196511266210693E-4	0.0
16	0.0	0.0	0.0	2.6495639082763366E-4	0.0
17	0.0	0.0	0.0	3.179476689931604E-4	0.0
18	0.0	0.0	0.0	3.7093894715868717E-4	0.0
19	0.0	0.0	0.0	4.769215034897406E-4	0.0
20	0.0	5.299127816552673E-5	0.0	5.829040598207941E-4	0.0
21	0.0	5.299127816552673E-5	0.0	7.94869172482901E-4	0.0
22	0.0	1.0598255633105347E-4	0.0	9.538430069794812E-4	0.0
23	0.0	1.0598255633105347E-4	0.0	0.002013668570290016	0.0
24	0.0	1.0598255633105347E-4	0.0	0.003603406915255818	0.0
25	0.0	1.0598255633105347E-4	0.0	0.004345284809573192	0.0
26	0.0	2.1196511266210693E-4	0.0	0.005564084207380307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	535	0.0	18.541443	29
TCTAGCG	560	0.0	17.999403	28
GTCCTAC	790	0.0	17.824556	1
TAGCGGC	565	0.0	17.556942	30
AACCGCG	280	0.0	17.144106	7
CGAACGA	150	5.456968E-12	17.066553	16
ACGTTTT	395	0.0	17.012096	29
TTATACC	115	1.843E-8	16.695541	4
ACGAACG	155	1.0913936E-11	16.51602	15
GTAGGAC	825	0.0	16.096437	3
GACGCGC	70	3.6989021E-4	15.999469	26
AGCGGCG	625	0.0	15.871474	31
TAGGACG	555	0.0	15.567464	4
CGCGGTC	310	0.0	15.484999	10
GAACCGC	345	0.0	15.305463	6
CGCCGGT	685	0.0	15.183588	7
TAGCGTA	370	0.0	15.136238	7
CGCGGTT	605	0.0	15.075479	10
CGGTCCA	690	0.0	15.073562	10
GTTTTCG	575	0.0	15.02559	28
>>END_MODULE