FastQCFastQC Report
Thu 2 Feb 2017
SRR4062969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200551
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39311.9600999247074309No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA38961.9426480047469223No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC33841.6873513470389079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28301.411112385378283No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG16750.8351990266814925No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15650.7803501353770362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15210.7584105788552538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.7394627800409871No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9530.4751908492104253No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8930.4452732721352674No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG8350.4163529476292813No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC8090.4033886642300462No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA7190.3585122986173093No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7040.35103290434851986No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6740.3360741158109408No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC6300.3141345592891584No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.28571286106775834No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5590.27873209308355484No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG5550.27673758794521097No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5090.2538007788542565No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.24332962687795123No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC4810.23983924288584949No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT4750.23684748517833368No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4680.23335710118623193No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.1989518875498003No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA3700.1844917252968073No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC3670.18299584644304942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC3390.16903431047464235No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3240.16155491620585286No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3210.16005903735209498No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC3160.15756590592916514No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA3080.15357689565247742No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC2990.14908925909120374No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2910.145100248814516No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG2740.1366236019765546No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2720.13562634940738266No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2710.1351277231227967No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2630.13113871284610895No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2620.130640086561523No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2600.12964283399235108No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA2580.12864558142317914No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2500.12465657114649141No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG2470.12316069229273352No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2410.12016893458521774No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG2390.1191716820160458No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2280.11368679288560016No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT2250.11219091403184228No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGCA2080.10371426719388087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.1032156409092949No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2060.10271701462470893No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATC2010.1002238832017791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGC200.003730914332.0267032
CACTGGA308.351727E-426.6889212
AGCACCG308.4005645E-426.6623045
ACCGGGC308.4005645E-426.6623048
TAGGAAC308.4005645E-426.6623044
TTGTAGA805.1077222E-922.0293541
ATACACT1151.8189894E-1220.8661524
ACCTAAA400.004436420320.0266861
ACTGTTC1052.746674E-1019.8062828
TCAATGT507.1635004E-419.20164530
TACCACA507.175537E-419.1968620
CTGTTCT1105.11136E-1018.9107139
TATACAC1208.185452E-1118.6636123
TGTAGAA1550.018.596152
GATATAC1301.2732926E-1118.486171
AACAGTG1055.9026206E-918.2827227
TTAGGAC1850.018.1591913
CACTGTT1251.4551915E-1017.9170687
CCCTATA450.00875796317.8014981
ACTCCTG450.00882117417.77930315
Produced by FastQC (version 0.11.5)