##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062969_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200551
Sequences flagged as poor quality	0
Sequence length	38
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.996175536397224	32.0	32.0	32.0	21.0	32.0
2	29.386141181046217	32.0	32.0	32.0	14.0	32.0
3	29.686752995497404	32.0	32.0	32.0	21.0	32.0
4	29.523108835159135	32.0	32.0	32.0	21.0	32.0
5	29.70961999690852	32.0	32.0	32.0	21.0	32.0
6	33.275401269502524	36.0	36.0	36.0	21.0	36.0
7	32.7844239121221	36.0	32.0	36.0	21.0	36.0
8	32.83946726767755	36.0	36.0	36.0	21.0	36.0
9	32.75729864224063	36.0	32.0	36.0	21.0	36.0
10	32.39838744259565	36.0	32.0	36.0	21.0	36.0
11	33.1416946312908	36.0	36.0	36.0	21.0	36.0
12	32.7520331486754	36.0	32.0	36.0	21.0	36.0
13	32.92456282940499	36.0	32.0	36.0	21.0	36.0
14	32.73864004667142	36.0	32.0	36.0	21.0	36.0
15	32.857667127064936	36.0	32.0	36.0	21.0	36.0
16	32.85780674242462	36.0	32.0	36.0	21.0	36.0
17	32.70556117895199	36.0	32.0	36.0	21.0	36.0
18	32.85757737433371	36.0	32.0	36.0	21.0	36.0
19	32.59129099331342	36.0	32.0	36.0	21.0	36.0
20	32.613589560760104	36.0	32.0	36.0	21.0	36.0
21	32.32924293571212	36.0	32.0	36.0	14.0	36.0
22	32.238233666249485	36.0	32.0	36.0	14.0	36.0
23	32.215815428494494	36.0	32.0	36.0	14.0	36.0
24	32.137745511116876	36.0	32.0	36.0	14.0	36.0
25	32.26666035073373	36.0	32.0	36.0	14.0	36.0
26	32.071876978923065	36.0	32.0	36.0	14.0	36.0
27	32.045634277565306	36.0	32.0	36.0	14.0	36.0
28	32.051722504500106	36.0	32.0	36.0	14.0	36.0
29	31.91738261090695	36.0	32.0	36.0	14.0	36.0
30	31.85130465567362	36.0	32.0	36.0	14.0	36.0
31	31.81480022537908	36.0	32.0	36.0	14.0	36.0
32	31.698395919242486	36.0	32.0	36.0	14.0	36.0
33	31.518272160198652	36.0	32.0	36.0	14.0	36.0
34	31.3383528379315	36.0	32.0	36.0	14.0	36.0
35	31.244840464520248	36.0	32.0	36.0	14.0	36.0
36	31.12724942782634	36.0	32.0	36.0	14.0	36.0
37	31.05312364435979	36.0	32.0	36.0	14.0	36.0
38	29.8634910820689	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	190.0
15	491.0
16	649.0
17	905.0
18	1254.0
19	1608.0
20	1956.0
21	2186.0
22	2485.0
23	2848.0
24	3222.0
25	3848.0
26	4439.0
27	5516.0
28	6924.0
29	9026.0
30	10978.0
31	13381.0
32	17189.0
33	23224.0
34	38521.0
35	49708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.642036944583126	16.819770344483274	13.799800299550673	19.738392411382925
2	11.427630659934945	23.13813334397637	42.59543812735727	22.838797868731415
3	20.824211717889003	32.26832759024789	26.584917045244875	20.32254364661823
4	10.934593885722549	17.778874777483583	34.96187926022329	36.32465207657058
5	10.605282446858903	42.357804249293196	31.80088855203913	15.236024751808767
6	28.25366116349457	34.79563801726244	21.481817592532572	15.468883226710412
7	25.730612163489585	33.95545272773509	21.685257116643648	18.628677992131674
8	29.898428812908566	31.684525976195342	21.03525821619654	17.38178699469955
9	30.406881077038143	15.097481924707058	18.94988780852655	35.54574918972825
10	17.045210896090232	29.672548853397423	31.28611960169335	21.99612064881899
11	33.47361492702532	24.094859610369536	21.322469820343155	21.10905564226199
12	25.081650868366335	26.789961555530066	30.50825484046293	17.620132735640667
13	33.4378880284043	22.822677564291162	25.541930754539155	18.19750365276538
14	25.16555300688142	21.197267378079186	28.27216515408397	25.365014460955422
15	24.735177944462176	27.840229013724866	30.714371209703163	16.7102218321098
16	20.89599872342123	27.566122790920332	31.75040890413691	19.78746958152152
17	19.47834326608982	26.766576066628435	31.807096725930727	21.94798394135102
18	21.0620007379853	24.681619179639583	36.79255632124301	17.46382376113211
19	21.211018140838327	29.349961604053014	31.060825163806083	18.378195091302572
20	23.12736230091849	22.929901369261916	36.64296470635166	17.299771623467933
21	23.957834689644166	25.85218206478379	27.399473432264244	22.790509813307803
22	21.165641112584897	28.88771429426255	29.566873772077667	20.379770821074885
23	19.342870962755374	28.982392452340942	29.790215369578988	21.8845212153247
24	19.155546246983025	30.571180659445872	31.10027327309357	19.17299982047753
25	22.923284842984025	26.699447938121196	29.452775519770995	20.92449169912378
26	17.11233680094952	28.155514108177655	32.80587666191241	21.926272428960413
27	21.07470367359965	29.827115922588625	31.863627523823297	17.23455287998843
28	18.874162278602203	28.025769905856073	30.929372107866605	22.170695707675122
29	17.602150215919178	30.09105506188353	33.13636318303763	19.17043153915966
30	20.815361831771785	28.55461757475729	32.14077357653242	18.489247016938503
31	18.11033045282059	29.597642400881607	30.86520097934109	21.42682616695671
32	17.176623117582526	32.02104318340481	31.110501645556997	19.69183205345567
33	17.047058178209724	31.401373298959307	31.460214721326025	20.091353801504944
34	16.532605931252526	32.86274940034209	32.88369379297964	17.720950875425746
35	19.07302866489797	31.894935269593667	31.446609882108874	17.585426183399495
36	17.698951756879406	33.92727127654269	30.867318951158452	17.506458015419447
37	18.79783982168057	33.42990640224595	30.065972204907776	17.706281571165707
38	17.77224834574131	35.49413342774368	27.607046867754047	19.126571358760962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	300.5
2	318.0
3	318.0
4	809.5
5	1301.0
6	1301.0
7	1620.0
8	1939.0
9	1791.5
10	1644.0
11	1644.0
12	1871.0
13	2098.0
14	2555.0
15	3012.0
16	3012.0
17	4081.5
18	5151.0
19	5151.0
20	5500.0
21	5849.0
22	4743.5
23	3638.0
24	3638.0
25	3682.5
26	3727.0
27	3727.0
28	5312.5
29	6898.0
30	8547.5
31	10197.0
32	10197.0
33	10374.0
34	10551.0
35	10551.0
36	10830.0
37	11109.0
38	11986.5
39	12864.0
40	12864.0
41	13242.5
42	13621.0
43	14817.0
44	16013.0
45	16013.0
46	22953.5
47	29894.0
48	29894.0
49	24612.5
50	19331.0
51	14751.0
52	10171.0
53	10171.0
54	8748.0
55	7325.0
56	7325.0
57	6929.5
58	6534.0
59	5709.5
60	4885.0
61	4885.0
62	4308.0
63	3731.0
64	3184.0
65	2637.0
66	2637.0
67	2299.5
68	1962.0
69	1962.0
70	1700.0
71	1438.0
72	1236.5
73	1035.0
74	1035.0
75	814.5
76	594.0
77	594.0
78	475.5
79	357.0
80	257.5
81	158.0
82	158.0
83	133.0
84	108.0
85	108.0
86	79.5
87	51.0
88	34.0
89	17.0
90	17.0
91	12.5
92	8.0
93	9.0
94	10.0
95	10.0
96	6.5
97	3.0
98	3.0
99	46.0
100	89.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1251551974310774
2	0.053353012450698324
3	0.009972525691719313
4	0.001994505138343863
5	0.0
6	0.0
7	0.0
8	9.972525691719315E-4
9	4.986262845859657E-4
10	9.972525691719315E-4
11	9.972525691719315E-4
12	9.972525691719315E-4
13	0.008975273122547383
14	0.005484889130445623
15	0.019446425098852662
16	0.007479394268789486
17	0.017950546245094765
18	0.0024931314229298283
19	0.004487636561273691
20	0.0024931314229298283
21	0.00349038399210176
22	0.004487636561273691
23	0.005983515415031587
24	0.009473899407133349
25	0.014958788537578972
26	0.014460162252993004
27	0.005983515415031587
28	0.00349038399210176
29	0.006482141699617554
30	9.972525691719315E-4
31	0.003989010276687726
32	0.005484889130445623
33	0.005983515415031587
34	0.009972525691719313
35	0.013462909683821073
36	0.01246565711464914
37	0.005983515415031587
38	0.003989010276687726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	200551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.03218134040718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6816872115947	35.29825331212509
2	12.631339500422452	11.62896220911389
3	4.480166381420742	6.186954939142662
4	2.036439264282155	3.749669660086462
5	1.1135423211074764	2.5629391027718635
6	0.7073376806256635	1.9536177830078132
7	0.45494919733963035	1.465961276682739
8	0.3097987391407959	1.1408569391326895
9	0.22855781104443337	0.9468913144287487
>10	1.156870816092203	9.649914485592195
>50	0.08774020234407158	2.8596217421005132
>100	0.08990662709330792	8.246780120767285
>500	0.01299854849541801	4.194942932221729
>1k	0.008665698996945342	10.114634182826315
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3931	1.9600999247074309	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3896	1.9426480047469223	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3384	1.6873513470389079	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2830	1.411112385378283	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1675	0.8351990266814925	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1565	0.7803501353770362	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1521	0.7584105788552538	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1483	0.7394627800409871	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	953	0.4751908492104253	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	893	0.4452732721352674	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	835	0.4163529476292813	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	809	0.4033886642300462	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	719	0.3585122986173093	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	704	0.35103290434851986	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	674	0.3360741158109408	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	630	0.3141345592891584	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	0.28571286106775834	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	559	0.27873209308355484	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	555	0.27673758794521097	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	509	0.2538007788542565	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	488	0.24332962687795123	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	481	0.23983924288584949	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	475	0.23684748517833368	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	468	0.23335710118623193	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.1989518875498003	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	370	0.1844917252968073	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	367	0.18299584644304942	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	339	0.16903431047464235	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	324	0.16155491620585286	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	321	0.16005903735209498	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	316	0.15756590592916514	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	308	0.15357689565247742	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	299	0.14908925909120374	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	291	0.145100248814516	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	274	0.1366236019765546	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	272	0.13562634940738266	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	271	0.1351277231227967	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	263	0.13113871284610895	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	262	0.130640086561523	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	260	0.12964283399235108	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA	258	0.12864558142317914	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	250	0.12465657114649141	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	247	0.12316069229273352	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.12016893458521774	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	239	0.1191716820160458	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	228	0.11368679288560016	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	225	0.11219091403184228	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGCA	208	0.10371426719388087	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	207	0.1032156409092949	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	206	0.10271701462470893	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATC	201	0.1002238832017791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.986262845859656E-4	0.0
11	0.0	0.0	0.0	4.986262845859656E-4	0.0
12	0.0	0.0	0.0	9.972525691719313E-4	0.0
13	0.0	0.0	0.0	9.972525691719313E-4	0.0
14	0.0	0.0	0.0	9.972525691719313E-4	0.0
15	0.0	0.0	0.0	9.972525691719313E-4	0.0
16	0.0	0.0	0.0	0.001495878853757897	0.0
17	0.0	0.0	0.0	0.001495878853757897	0.0
18	0.0	0.0	0.0	0.0024931314229298283	0.0
19	0.0	0.0	0.0	0.0024931314229298283	0.0
20	0.0	0.0	0.0	0.0024931314229298283	0.0
21	0.0	0.0	0.0	0.004986262845859657	0.0
22	0.0	0.0	0.0	0.008476646837961416	0.0
23	0.0	0.0	0.0	0.015457414822164936	0.0
24	0.0	0.0	0.0	0.023435435375540388	0.0
25	0.0	0.0	0.0	0.029418950790571975	0.0
26	0.0	0.0	0.0	0.03689834505936146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGC	20	0.0037309143	32.026703	2
CACTGGA	30	8.351727E-4	26.688921	2
AGCACCG	30	8.4005645E-4	26.662304	5
ACCGGGC	30	8.4005645E-4	26.662304	8
TAGGAAC	30	8.4005645E-4	26.662304	4
TTGTAGA	80	5.1077222E-9	22.029354	1
ATACACT	115	1.8189894E-12	20.866152	4
ACCTAAA	40	0.0044364203	20.026686	1
ACTGTTC	105	2.746674E-10	19.806282	8
TCAATGT	50	7.1635004E-4	19.201645	30
TACCACA	50	7.175537E-4	19.19686	20
CTGTTCT	110	5.11136E-10	18.910713	9
TATACAC	120	8.185452E-11	18.663612	3
TGTAGAA	155	0.0	18.59615	2
GATATAC	130	1.2732926E-11	18.48617	1
AACAGTG	105	5.9026206E-9	18.282722	7
TTAGGAC	185	0.0	18.159191	3
CACTGTT	125	1.4551915E-10	17.917068	7
CCCTATA	45	0.008757963	17.801498	1
ACTCCTG	45	0.008821174	17.779303	15
>>END_MODULE