FastQCFastQC Report
Thu 2 Feb 2017
SRR4062969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200551
Sequences flagged as poor quality0
Sequence length38
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52912.6382316717443444No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA40672.0279130994111223No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC39951.9920120069209328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39601.974560086960424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21391.0665616227293806No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG19120.9533734561283664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.9005190699622541No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12640.6302636237166606No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC12550.6257759871553868No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG10190.508100183993099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10150.5061056788547551No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG9170.4572403029653305No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA8620.4298158573131024No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC8580.4278213521747586No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.39690652253042863No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7280.36299993517858303No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.34155900494138647No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6800.33906587351845663No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT6760.33707136838011276No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6660.33208510553425313No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC6180.3081510438741268No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6070.30266615474368114No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.3011702758899233No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA5300.2642719308305618No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC4990.24881451600839688No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4810.23983924288584949No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT4550.22687495948661437No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4260.21241479723362136No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG4100.20443677668024593No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3670.18299584644304942No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3550.1770123310280178No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3500.174519199605088No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC3450.17202606818215815No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3420.17053018932840025No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA3410.17003156304381428No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC3320.1655439264825406No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA3250.16205354249043882No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC3220.16055766363668095No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2760.1376208545457265No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG2720.13562634940738266No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2690.13413047055362476No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2660.13263459169986686No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2630.13113871284610895No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG2600.12964283399235108No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2580.12864558142317914No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2410.12016893458521774No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2360.1176758031622879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2340.11667855059311596No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2320.11568129802394403No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT2320.11568129802394403No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2320.11568129802394403No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2220.11069503517808438No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2210.11019640889349841No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2090.10421289347846684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2050.10221838834012296No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG2040.101719762055537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATC200.003746191532.017
TCATGCT200.003746191532.027
TAGGACC601.7482853E-724.04
ATAGGAC350.002060634822.8571433
TAGAAAT1051.2732926E-1121.3333344
TCCTCGC555.8025966E-520.36363629
TCACGTT951.6989361E-920.21052627
TGTATCA400.004470808420.018
TGGGCGT400.004470808420.06
AAAAGTA1300.019.6923082
AAAAAGT1300.019.6923081
TTTAGAA1154.3655746E-1119.478262
GAAATGT1154.3655746E-1119.478266
ATGTCCA1154.3655746E-1119.478269
CACGTTT1154.3655746E-1119.4782628
TTCCTCG507.1676384E-419.228
AAAGTAC1351.8189894E-1218.9629633
TGCTTAC601.1322431E-418.66666626
GTCCACT1208.185452E-1118.66666611
CGTTTTT1208.185452E-1118.66666630
Produced by FastQC (version 0.11.5)