##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200551
Sequences flagged as poor quality	0
Sequence length	38
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.453216388848723	32.0	32.0	32.0	32.0	32.0
2	31.090086810836148	32.0	32.0	32.0	32.0	32.0
3	31.249731988372034	32.0	32.0	32.0	32.0	32.0
4	31.376532652542245	32.0	32.0	32.0	32.0	32.0
5	31.18311551675135	32.0	32.0	32.0	32.0	32.0
6	34.66096903032147	36.0	36.0	36.0	32.0	36.0
7	34.7609386141181	36.0	36.0	36.0	32.0	36.0
8	34.73460616002912	36.0	36.0	36.0	32.0	36.0
9	34.77433670238493	36.0	36.0	36.0	32.0	36.0
10	34.66524724384321	36.0	36.0	36.0	32.0	36.0
11	34.819671804179485	36.0	36.0	36.0	32.0	36.0
12	34.74295316403309	36.0	36.0	36.0	32.0	36.0
13	34.748338327906616	36.0	36.0	36.0	32.0	36.0
14	34.67966252973059	36.0	36.0	36.0	32.0	36.0
15	34.66244995038669	36.0	36.0	36.0	32.0	36.0
16	34.62518262187673	36.0	36.0	36.0	32.0	36.0
17	34.605736196777876	36.0	36.0	36.0	32.0	36.0
18	34.57219360661378	36.0	36.0	36.0	32.0	36.0
19	34.48312399339819	36.0	36.0	36.0	32.0	36.0
20	34.429282327188595	36.0	36.0	36.0	32.0	36.0
21	34.33422919855797	36.0	36.0	36.0	32.0	36.0
22	34.30892890087808	36.0	36.0	36.0	32.0	36.0
23	34.20861027868223	36.0	36.0	36.0	32.0	36.0
24	34.220228271113086	36.0	36.0	36.0	32.0	36.0
25	34.22634641562495	36.0	36.0	36.0	32.0	36.0
26	34.13554656920185	36.0	36.0	36.0	32.0	36.0
27	34.08644185269582	36.0	36.0	36.0	32.0	36.0
28	33.958628977167905	36.0	36.0	36.0	32.0	36.0
29	33.89123963480611	36.0	36.0	36.0	32.0	36.0
30	33.80481772716167	36.0	36.0	36.0	32.0	36.0
31	33.765216827639854	36.0	36.0	36.0	32.0	36.0
32	33.63750367736885	36.0	36.0	36.0	27.0	36.0
33	33.51113183180338	36.0	36.0	36.0	21.0	36.0
34	33.34756745167065	36.0	36.0	36.0	21.0	36.0
35	33.177954734705885	36.0	36.0	36.0	14.0	36.0
36	33.032360845869626	36.0	36.0	36.0	14.0	36.0
37	32.90091797098992	36.0	36.0	36.0	14.0	36.0
38	32.32796146616073	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	9.0
22	28.0
23	72.0
24	207.0
25	492.0
26	1086.0
27	2134.0
28	3757.0
29	5875.0
30	8959.0
31	12988.0
32	17741.0
33	25634.0
34	48693.0
35	72873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.26761378086058	18.086756192062392	14.633063892808881	20.01256613426815
2	11.199146351800788	24.41723052989015	41.02397893802574	23.359644180283322
3	20.342456532253642	31.98188989334384	27.69769285618122	19.9779607182213
4	10.785785161879023	18.389337375530413	35.2234593694372	35.60141809315336
5	10.308599807530253	42.13092928980659	31.52714272180144	16.033328180861727
6	28.640595160333284	35.68020104611794	19.915632432648053	15.76357136090072
7	24.517953039376515	34.11102412852591	22.049254304391404	19.32176852770617
8	29.499025175892175	31.619703912759473	21.206288737415793	17.674982173932555
9	29.53437116671487	14.128429387771385	19.498269723654424	36.83892972185932
10	16.644228371977064	29.61655447519322	31.57466965843929	22.164547494390426
11	33.37704754543868	23.640080779874843	21.650502381011744	21.332369293674734
12	24.61543064288528	26.821375324733605	31.070710898583386	17.492483133797727
13	33.79339918524465	22.56483388265329	26.066686279300527	17.57508065280153
14	25.311267458152788	20.46860898225389	28.912346485432632	25.307777074160686
15	24.58825934550314	27.661791763691028	31.12774306784808	16.62220582295775
16	20.740858933637828	27.094355051832203	32.14244755698052	20.022338457549452
17	19.39706109667865	26.077656057561416	32.80163150520317	21.723651340556767
18	21.059481129488262	23.036534347871616	38.34386265837617	17.56012186426395
19	21.61096180024034	27.767999162307845	32.811604030894884	17.809435006556935
20	22.839576965460157	21.87623098364007	38.40768682280318	16.876505228096594
21	23.33770462376154	25.007604050839937	29.323713170216053	22.33097815518247
22	20.78523667296598	27.384056923176647	31.718615215082448	20.112091188774926
23	19.049114933931687	26.838194963849414	31.867863375716777	22.24482672650212
24	19.02159550438542	28.8460291895827	32.9816355939387	19.150739712093184
25	22.953263758345756	24.740838988586443	31.16663591804578	21.139261335022013
26	17.148256553195946	26.092614846099	34.85148416113607	21.907644439568987
27	21.658331297276003	27.34815583068646	33.98886068880235	17.004652183235187
28	18.96574936051179	25.28533889135432	33.633340147892554	22.115571600241335
29	18.13653384924533	27.24892920005385	35.18257201410115	19.43196493659967
30	21.150231113282906	26.064691774162185	34.2571216299096	18.52795548264531
31	18.187892356557683	26.94277266131807	33.0205284441364	21.848806537987844
32	17.637907564659365	29.10980249412868	33.582978893149374	19.66931104806259
33	17.720679527900636	28.157924916854064	33.84675219769535	20.274643357549948
34	17.183160393116964	29.72460870302317	35.208001954615035	17.88422894924483
35	20.135526624150465	29.070411017646386	32.93077571291093	17.86328664529222
36	18.71344446051129	30.549835204012943	32.94274274374099	17.793977591734773
37	20.21730133482256	30.01431057436762	31.98188989334384	17.78649819746598
38	19.441438836006803	30.647565955791794	29.861232304999724	20.049762903201678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	145.0
2	178.0
3	178.0
4	641.5
5	1105.0
6	1105.0
7	1451.0
8	1797.0
9	1776.0
10	1755.0
11	1755.0
12	2199.5
13	2644.0
14	3038.0
15	3432.0
16	3432.0
17	4892.0
18	6352.0
19	6352.0
20	6572.0
21	6792.0
22	5312.5
23	3833.0
24	3833.0
25	3560.5
26	3288.0
27	3288.0
28	4601.5
29	5915.0
30	7577.5
31	9240.0
32	9240.0
33	9648.0
34	10056.0
35	10056.0
36	10251.0
37	10446.0
38	11176.0
39	11906.0
40	11906.0
41	11777.0
42	11648.0
43	12415.5
44	13183.0
45	13183.0
46	22624.5
47	32066.0
48	32066.0
49	27085.0
50	22104.0
51	16591.0
52	11078.0
53	11078.0
54	9292.5
55	7507.0
56	7507.0
57	7150.0
58	6793.0
59	5889.5
60	4986.0
61	4986.0
62	4395.0
63	3804.0
64	3279.5
65	2755.0
66	2755.0
67	2369.5
68	1984.0
69	1984.0
70	1741.5
71	1499.0
72	1272.0
73	1045.0
74	1045.0
75	811.5
76	578.0
77	578.0
78	462.0
79	346.0
80	246.5
81	147.0
82	147.0
83	123.5
84	100.0
85	100.0
86	70.0
87	40.0
88	30.0
89	20.0
90	20.0
91	14.0
92	8.0
93	6.0
94	4.0
95	4.0
96	4.0
97	4.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005983515415031587
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	9.972525691719315E-4
9	0.0024931314229298283
10	4.986262845859657E-4
11	0.0029917577075157936
12	9.972525691719315E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	4.986262845859657E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	200551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.653988262337265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3235215038051	32.12848602101211
2	13.428334288018892	11.455440262077975
3	4.8197982301298765	6.16750851404381
4	2.1053739055212	3.592103754157297
5	1.1386086529581614	2.428310005933653
6	0.7656967840735069	1.9596012984228453
7	0.4325310077972482	1.2914420770776511
8	0.33784178717136415	1.1528239699627527
9	0.24432156926925638	0.9379160413062014
>10	1.1725097319476754	8.85061655140089
>50	0.10871725331120022	3.2500461229313244
>100	0.09468922062588406	8.361464166222058
>500	0.015197035409092504	4.600824727874706
>1k	0.011690027237763464	11.18518481583238
>5k	0.0011690027237763463	2.6382316717443444
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5291	2.6382316717443444	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4067	2.0279130994111223	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3995	1.9920120069209328	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3960	1.974560086960424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2139	1.0665616227293806	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1912	0.9533734561283664	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1806	0.9005190699622541	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1264	0.6302636237166606	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	1255	0.6257759871553868	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	1019	0.508100183993099	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1015	0.5061056788547551	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	917	0.4572403029653305	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	862	0.4298158573131024	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	858	0.4278213521747586	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	796	0.39690652253042863	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	728	0.36299993517858303	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	685	0.34155900494138647	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	680	0.33906587351845663	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	676	0.33707136838011276	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	666	0.33208510553425313	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	618	0.3081510438741268	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	607	0.30266615474368114	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.3011702758899233	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	530	0.2642719308305618	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	499	0.24881451600839688	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	481	0.23983924288584949	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	455	0.22687495948661437	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	426	0.21241479723362136	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	410	0.20443677668024593	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	367	0.18299584644304942	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	355	0.1770123310280178	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	350	0.174519199605088	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	345	0.17202606818215815	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	342	0.17053018932840025	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	341	0.17003156304381428	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	332	0.1655439264825406	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	325	0.16205354249043882	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	322	0.16055766363668095	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	276	0.1376208545457265	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	272	0.13562634940738266	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	269	0.13413047055362476	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	266	0.13263459169986686	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	263	0.13113871284610895	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	260	0.12964283399235108	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	258	0.12864558142317914	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	241	0.12016893458521774	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	236	0.1176758031622879	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	234	0.11667855059311596	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	232	0.11568129802394403	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	232	0.11568129802394403	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	232	0.11568129802394403	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	222	0.11069503517808438	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	221	0.11019640889349841	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	209	0.10421289347846684	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	205	0.10221838834012296	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG	204	0.101719762055537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.986262845859656E-4	0.0
9	0.0	0.0	0.0	4.986262845859656E-4	0.0
10	0.0	0.0	0.0	0.001495878853757897	0.0
11	0.0	0.0	0.0	0.001495878853757897	0.0
12	0.0	0.0	0.0	0.0019945051383438626	0.0
13	0.0	0.0	0.0	0.0019945051383438626	0.0
14	0.0	0.0	0.0	0.0019945051383438626	0.0
15	0.0	0.0	0.0	0.0019945051383438626	0.0
16	0.0	0.0	0.0	0.0024931314229298283	0.0
17	0.0	0.0	0.0	0.0024931314229298283	0.0
18	0.0	0.0	0.0	0.003989010276687725	0.0
19	0.0	0.0	0.0	0.003989010276687725	0.0
20	0.0	0.0	0.0	0.003989010276687725	0.0
21	0.0	0.0	0.0	0.00698076798420352	0.0
22	0.0	0.0	0.0	0.01047115197630528	0.0
23	0.0	0.0	0.0	0.017451919960508797	0.0
24	0.0	0.0	0.0	0.02542994051388425	0.0
25	0.0	0.0	0.0	0.03141345592891584	0.0
26	0.0	0.0	0.0	0.038892850197705324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTATC	20	0.0037461915	32.0	17
TCATGCT	20	0.0037461915	32.0	27
TAGGACC	60	1.7482853E-7	24.0	4
ATAGGAC	35	0.0020606348	22.857143	3
TAGAAAT	105	1.2732926E-11	21.333334	4
TCCTCGC	55	5.8025966E-5	20.363636	29
TCACGTT	95	1.6989361E-9	20.210526	27
TGTATCA	40	0.0044708084	20.0	18
TGGGCGT	40	0.0044708084	20.0	6
AAAAGTA	130	0.0	19.692308	2
AAAAAGT	130	0.0	19.692308	1
TTTAGAA	115	4.3655746E-11	19.47826	2
GAAATGT	115	4.3655746E-11	19.47826	6
ATGTCCA	115	4.3655746E-11	19.47826	9
CACGTTT	115	4.3655746E-11	19.47826	28
TTCCTCG	50	7.1676384E-4	19.2	28
AAAGTAC	135	1.8189894E-12	18.962963	3
TGCTTAC	60	1.1322431E-4	18.666666	26
GTCCACT	120	8.185452E-11	18.666666	11
CGTTTTT	120	8.185452E-11	18.666666	30
>>END_MODULE