FastQCFastQC Report
Thu 2 Feb 2017
SRR4062968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480689
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA61940.41831876916759697No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA57510.38840026501176145No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC54600.36874725212384235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48580.32809050381275207No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG47780.32268761367174337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32530.21969502035876542No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA28330.19132984711846984No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA27500.18572434859717335No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA26690.18025392232940204No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA23250.15702149472306473No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA18600.1256171957784518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18140.1225105339473718No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT16820.11359576521470748No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16550.11177228979211704No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15940.10765258605959793No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15290.10326273782002837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC805.2586984E-921.99927931
GGCGATA1051.2732926E-1121.33191519
TCTAGCG2750.020.36228228
TAGGACC22450.020.3077094
GCGATAA1154.5474735E-1119.47762320
AGCGGCG2900.019.30971331
CTACTAG507.167834E-419.21171
CTAGCGG3000.019.19937329
AATCCGC507.198863E-419.19937130
AGGACCT31150.019.1556935
CGATAAA1105.2023097E-1018.90847221
TAGCGGC3000.018.66605830
GGACCTG31500.018.130296
CAAGACG3450.018.083314
TGTAGGA52150.018.0782952
GGCATCG1600.017.99880226
GCATCGT1600.017.99819427
GCGGCGC3150.017.77719732
AAATCCG450.00885311117.77719529
CAACGAT450.00885311117.77719530
Produced by FastQC (version 0.11.5)