##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062968_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1480689
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36321739406452	32.0	32.0	32.0	32.0	32.0
2	31.089999993246387	32.0	32.0	32.0	32.0	32.0
3	31.12936410009124	32.0	32.0	32.0	32.0	32.0
4	31.18002700094348	32.0	32.0	32.0	32.0	32.0
5	31.150901370915836	32.0	32.0	32.0	32.0	32.0
6	34.83572715134643	36.0	36.0	36.0	36.0	36.0
7	34.76587926296474	36.0	36.0	36.0	32.0	36.0
8	34.76022243698711	36.0	36.0	36.0	32.0	36.0
9	34.85738666256047	36.0	36.0	36.0	32.0	36.0
10	34.649617846826715	36.0	36.0	36.0	32.0	36.0
11	34.84635801306013	36.0	36.0	36.0	36.0	36.0
12	34.70321114021918	36.0	36.0	36.0	32.0	36.0
13	34.76335341182382	36.0	36.0	36.0	32.0	36.0
14	34.69966279211907	36.0	36.0	36.0	32.0	36.0
15	34.66128201127989	36.0	36.0	36.0	32.0	36.0
16	34.68840114298141	36.0	36.0	36.0	32.0	36.0
17	34.643855664491326	36.0	36.0	36.0	32.0	36.0
18	34.670674935790025	36.0	36.0	36.0	32.0	36.0
19	34.617588838709544	36.0	36.0	36.0	32.0	36.0
20	34.600810838737914	36.0	36.0	36.0	32.0	36.0
21	34.566676054188285	36.0	36.0	36.0	32.0	36.0
22	34.53362252302813	36.0	36.0	36.0	32.0	36.0
23	34.52381087453206	36.0	36.0	36.0	32.0	36.0
24	34.501340929796875	36.0	36.0	36.0	32.0	36.0
25	34.46707512516133	36.0	36.0	36.0	32.0	36.0
26	34.4606754017893	36.0	36.0	36.0	32.0	36.0
27	34.41310092801392	36.0	36.0	36.0	32.0	36.0
28	34.362948600280006	36.0	36.0	36.0	32.0	36.0
29	34.30670924144098	36.0	36.0	36.0	32.0	36.0
30	34.256051068117614	36.0	36.0	36.0	32.0	36.0
31	34.261732207100884	36.0	36.0	36.0	32.0	36.0
32	34.22720098548716	36.0	36.0	36.0	32.0	36.0
33	34.241920484315074	36.0	36.0	36.0	32.0	36.0
34	34.22947762832033	36.0	36.0	36.0	32.0	36.0
35	34.209393059582396	36.0	36.0	36.0	32.0	36.0
36	34.13648510929709	36.0	36.0	36.0	32.0	36.0
37	34.0718483084564	36.0	36.0	36.0	32.0	36.0
38	33.51638257594944	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	2.0
9	3.0
10	14.0
11	18.0
12	5.0
13	8.0
14	150.0
15	357.0
16	514.0
17	540.0
18	567.0
19	767.0
20	1014.0
21	1372.0
22	1977.0
23	2900.0
24	4346.0
25	6725.0
26	10333.0
27	15363.0
28	22366.0
29	30715.0
30	43173.0
31	59233.0
32	83165.0
33	129079.0
34	314982.0
35	750997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.413767688180876	20.259817713774517	12.491184224850782	24.835230373193824
2	12.474432100375504	20.64548346051681	46.43164358263457	20.448440856473113
3	18.02186015284005	27.37429601581085	29.845484842303488	24.758358989045608
4	10.26435029503954	16.953241450201904	40.594293448345965	32.18811480641259
5	10.73841548905776	40.727798774080085	36.11851009398359	12.415275642878562
6	29.230282969999738	39.78585616001221	19.313129648602303	11.670731221385749
7	27.12821818520727	32.44412897526621	23.043087431712443	17.384565407814076
8	23.701645593044287	40.87204409859781	20.340829428601147	15.08548087975676
9	27.5388266503859	14.675109611503872	21.39916417007275	36.38689956803747
10	15.047525397080536	29.05330111394614	34.68651607840171	21.212657410571616
11	34.00010805991923	21.496292194021585	27.566625693947294	16.936974052111896
12	25.393914834700976	24.68084962685307	33.09654543612602	16.828690102319925
13	29.704940488935687	23.13565658728481	26.472274889039443	20.68712803474006
14	19.457502566596425	25.029110606797428	27.719916788242287	27.79347003836386
15	20.3506901802156	37.34403899649944	23.000693084957625	19.30457773832733
16	19.24786800473882	28.285055837810308	32.86096000237752	19.60611615507336
17	18.933803661752627	31.542829428040292	30.00349909212745	19.519867818079632
18	19.630707260636466	26.647661037920756	35.88734133271737	17.83429036872541
19	23.7232156425713	26.09553720682843	28.5056819141212	21.675565236479073
20	24.412556708007706	27.457448310190863	29.836331082235645	18.293663899565786
21	22.375764305247962	26.790549462276264	26.81087915450093	24.02280707797484
22	23.200091315010447	31.488954733555364	27.492099427997914	17.818854523436283
23	18.678198378310835	30.93670954473154	30.629054645048647	19.75603743190898
24	22.841578407003134	27.121001296876692	31.50322868258943	18.534191613530744
25	20.34496151520985	29.27613470183153	30.974396384895186	19.404507398063433
26	17.6366721516559	31.257143320336883	31.96721975893067	19.13896476907655
27	19.604867327765195	30.384461077722978	29.90410424121586	20.106567353295972
28	18.17421685624033	30.81318895071805	28.68392134826149	22.328672844780133
29	20.47190112676475	28.945736706377883	28.24632122994457	22.33604093691279
30	18.511379492464776	29.051846288714522	31.005115334793054	21.431658884027645
31	22.845732394252042	27.422402870195516	28.944425082872034	20.787439652680405
32	19.649774042322406	30.339250581019556	29.53281367457637	20.478161702081675
33	18.005651862048097	30.671280192682026	32.218247787682344	19.10482015758753
34	20.14426051457735	30.046956601617275	31.805532665638196	18.00325021816718
35	19.668441561862743	30.670134951025457	31.07703468441967	18.584388802692132
36	17.97178745214377	29.802779646546114	32.55324638832376	19.672186512986364
37	19.276012292729053	29.6778224308534	28.42315355779947	22.62301171861808
38	19.47746612337084	32.263221560550406	28.417397623789924	19.841914692288835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	143.5
2	202.0
3	202.0
4	537.5
5	873.0
6	873.0
7	1101.5
8	1330.0
9	1330.0
10	1330.0
11	1330.0
12	1871.5
13	2413.0
14	3545.5
15	4678.0
16	4678.0
17	7160.5
18	9643.0
19	9643.0
20	12574.5
21	15506.0
22	18829.5
23	22153.0
24	22153.0
25	30034.5
26	37916.0
27	37916.0
28	51390.0
29	64864.0
30	86990.0
31	109116.0
32	109116.0
33	134860.0
34	160604.0
35	160604.0
36	169413.0
37	178222.0
38	178950.5
39	179679.0
40	179679.0
41	161562.0
42	143445.0
43	128565.5
44	113686.0
45	113686.0
46	101722.0
47	89758.0
48	89758.0
49	82930.0
50	76102.0
51	70179.5
52	64257.0
53	64257.0
54	56228.0
55	48199.0
56	48199.0
57	45140.0
58	42081.0
59	35753.0
60	29425.0
61	29425.0
62	29365.0
63	29305.0
64	23676.5
65	18048.0
66	18048.0
67	15630.0
68	13212.0
69	13212.0
70	10731.5
71	8251.0
72	6507.5
73	4764.0
74	4764.0
75	3639.5
76	2515.0
77	2515.0
78	2641.0
79	2767.0
80	2397.5
81	2028.0
82	2028.0
83	2067.0
84	2106.0
85	2106.0
86	1392.0
87	678.0
88	550.0
89	422.0
90	422.0
91	306.5
92	191.0
93	174.0
94	157.0
95	157.0
96	143.5
97	130.0
98	130.0
99	339.0
100	548.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12197024493327092
2	0.05443411817066244
3	0.012629255704607788
4	0.0027014450705043396
5	3.3768063381304245E-4
6	1.3507225352521696E-4
7	1.3507225352521696E-4
8	4.727528873382594E-4
9	3.3768063381304245E-4
10	4.727528873382594E-4
11	0.0019585476761156463
12	0.002633908943741731
13	0.014047514366622566
14	0.008712160352376494
15	0.02370518049367558
16	0.010197955141153882
17	0.02059851866259559
18	0.005065209507195636
19	0.007699118450937368
20	0.005267817887483462
21	0.006483468169210414
22	0.006753612676260849
23	0.009049840986189538
24	0.014115050493385175
25	0.015803453662450385
26	0.01924779612734342
27	0.008306943591800843
28	0.00580810690158433
29	0.011278533169355618
30	0.002904053450792165
31	0.006280859788922589
32	0.006888684929786065
33	0.007766654577699976
34	0.01168374992993127
35	0.015263164648349517
36	0.014385195000435606
37	0.00938752162000258
38	0.006483468169210414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1480689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.31561122043595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.87133949592118	48.071370870854125
2	10.65153515238689	12.20998495390028
3	2.542568548209444	4.371866112314422
4	0.9022063761514314	2.068420395843753
5	0.4635478012708826	1.3284262779864906
6	0.27897749942551225	0.959385953779323
7	0.1838252323439542	0.7375238884672671
8	0.14115627881610002	0.6472366718357637
9	0.10487477957331509	0.5409865883567742
>10	0.6772098191128786	7.634255028833067
>50	0.09148227668789234	3.678884699954663
>100	0.07911443494105451	9.485999025915515
>500	0.008029483017838712	3.2625163422684946
>1k	0.003778580243688806	3.825196524603064
>5k	3.5424189784582556E-4	1.1779466650869839
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	6194	0.41831876916759697	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	5751	0.38840026501176145	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	5460	0.36874725212384235	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4858	0.32809050381275207	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	4778	0.32268761367174337	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3253	0.21969502035876542	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2833	0.19132984711846984	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2750	0.18572434859717335	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2669	0.18025392232940204	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	2325	0.15702149472306473	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	1860	0.1256171957784518	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1814	0.1225105339473718	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	1682	0.11359576521470748	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1655	0.11177228979211704	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1594	0.10765258605959793	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1529	0.10326273782002837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.753612676260849E-5	0.0
6	0.0	0.0	0.0	6.753612676260849E-5	0.0
7	0.0	0.0	0.0	6.753612676260849E-5	0.0
8	0.0	0.0	0.0	6.753612676260849E-5	0.0
9	0.0	0.0	0.0	1.3507225352521698E-4	0.0
10	0.0	0.0	0.0	1.3507225352521698E-4	0.0
11	0.0	0.0	0.0	1.3507225352521698E-4	0.0
12	0.0	0.0	0.0	1.3507225352521698E-4	0.0
13	0.0	0.0	0.0	1.3507225352521698E-4	0.0
14	0.0	0.0	0.0	1.3507225352521698E-4	0.0
15	0.0	0.0	0.0	1.3507225352521698E-4	0.0
16	0.0	0.0	0.0	1.3507225352521698E-4	0.0
17	0.0	0.0	0.0	1.3507225352521698E-4	0.0
18	0.0	0.0	0.0	2.7014450705043397E-4	0.0
19	0.0	0.0	0.0	3.3768063381304245E-4	0.0
20	0.0	0.0	0.0	6.078251408634764E-4	0.0
21	0.0	0.0	0.0	6.078251408634764E-4	0.0
22	0.0	0.0	0.0	8.104335211513019E-4	0.0
23	0.0	0.0	0.0	8.779696479139104E-4	0.0
24	0.0	0.0	0.0	0.0017559392958278208	0.0
25	0.0	0.0	0.0	0.0022962283099286886	0.0
26	0.0	0.0	0.0	0.0028365173240295564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGCC	80	5.2586984E-9	21.999279	31
GGCGATA	105	1.2732926E-11	21.331915	19
TCTAGCG	275	0.0	20.362282	28
TAGGACC	2245	0.0	20.307709	4
GCGATAA	115	4.5474735E-11	19.477623	20
AGCGGCG	290	0.0	19.309713	31
CTACTAG	50	7.167834E-4	19.2117	1
CTAGCGG	300	0.0	19.199373	29
AATCCGC	50	7.198863E-4	19.199371	30
AGGACCT	3115	0.0	19.155693	5
CGATAAA	110	5.2023097E-10	18.908472	21
TAGCGGC	300	0.0	18.666058	30
GGACCTG	3150	0.0	18.13029	6
CAAGACG	345	0.0	18.08331	4
TGTAGGA	5215	0.0	18.078295	2
GGCATCG	160	0.0	17.998802	26
GCATCGT	160	0.0	17.998194	27
GCGGCGC	315	0.0	17.777197	32
AAATCCG	45	0.008853111	17.777195	29
CAACGAT	45	0.008853111	17.777195	30
>>END_MODULE