FastQCFastQC Report
Thu 2 Feb 2017
SRR4062968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480689
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA65140.43993032973163165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53680.36253392846168236No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA52810.3566582854333354No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG51080.3449745355034041No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC50060.33808585057361806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36860.2489381632469749No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA29010.19592230373832722No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA27640.18666985437184985No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA26380.1781603023997612No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA25550.1725548038784647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20860.14088036042680133No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT17240.11643228253873704No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA17090.11541924063729792No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA16130.1089357724680875No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15870.10717983317225968No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15600.10535635774966924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.10481606873556837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2550.020.70459429
GCGCAAG2750.020.3644311
TAGCGGC2600.020.30642730
AAGACGG2800.020.0041585
GCGGTAC400.004485695619.99875626
AGCGGCG2650.019.92328831
CAAGACG2850.019.651884
TAGGACC20700.019.55834
TCTAGCG2750.019.19880528
CCTCGAC751.5100668E-619.19880330
GCGGCGC2800.018.85596832
GGCATCG2550.018.82235726
AGGACCT29050.018.7302285
ACCGTCG2400.018.6705478
CCGTCGT2400.018.6686559
CGCAAGA3000.018.6673952
GTCGTAG2400.018.66676511
TACCGTC2500.018.563867
AGGCCCG3500.018.2858110
GGACCTG28750.018.2020456
Produced by FastQC (version 0.11.5)