##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1480689
Sequences flagged as poor quality	0
Sequence length	38
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240459677893195	32.0	32.0	32.0	32.0	32.0
2	31.40044330713607	32.0	32.0	32.0	32.0	32.0
3	31.461363594921014	32.0	32.0	32.0	32.0	32.0
4	31.547769990862363	32.0	32.0	32.0	32.0	32.0
5	31.509408120138666	32.0	32.0	32.0	32.0	32.0
6	35.04941550859093	36.0	36.0	36.0	36.0	36.0
7	35.071412700438785	36.0	36.0	36.0	36.0	36.0
8	35.00160060620427	36.0	36.0	36.0	36.0	36.0
9	35.10223889013831	36.0	36.0	36.0	36.0	36.0
10	34.990754979607466	36.0	36.0	36.0	36.0	36.0
11	35.12409898364883	36.0	36.0	36.0	36.0	36.0
12	35.052322938848064	36.0	36.0	36.0	36.0	36.0
13	35.09061659808373	36.0	36.0	36.0	36.0	36.0
14	35.04951681278108	36.0	36.0	36.0	36.0	36.0
15	35.03362961432144	36.0	36.0	36.0	36.0	36.0
16	35.05107959875436	36.0	36.0	36.0	36.0	36.0
17	35.037280617334225	36.0	36.0	36.0	36.0	36.0
18	35.03962952382303	36.0	36.0	36.0	36.0	36.0
19	35.0049044735255	36.0	36.0	36.0	36.0	36.0
20	34.99732421865767	36.0	36.0	36.0	36.0	36.0
21	34.96522970049754	36.0	36.0	36.0	36.0	36.0
22	34.951386820595005	36.0	36.0	36.0	36.0	36.0
23	34.91764509630314	36.0	36.0	36.0	36.0	36.0
24	34.90076984430897	36.0	36.0	36.0	36.0	36.0
25	34.86807695606572	36.0	36.0	36.0	32.0	36.0
26	34.82101305540866	36.0	36.0	36.0	32.0	36.0
27	34.77561459563757	36.0	36.0	36.0	32.0	36.0
28	34.72677044267905	36.0	36.0	36.0	32.0	36.0
29	34.69089727822656	36.0	36.0	36.0	32.0	36.0
30	34.645083471275875	36.0	36.0	36.0	32.0	36.0
31	34.6387722202299	36.0	36.0	36.0	32.0	36.0
32	34.61629079435317	36.0	36.0	36.0	32.0	36.0
33	34.626752140388696	36.0	36.0	36.0	32.0	36.0
34	34.60682155401978	36.0	36.0	36.0	32.0	36.0
35	34.57596362234068	36.0	36.0	36.0	32.0	36.0
36	34.523818303506005	36.0	36.0	36.0	32.0	36.0
37	34.46592768636763	36.0	36.0	36.0	32.0	36.0
38	34.08056857314399	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	13.0
22	98.0
23	244.0
24	760.0
25	1856.0
26	4149.0
27	8089.0
28	14646.0
29	23784.0
30	36906.0
31	53825.0
32	76880.0
33	123295.0
34	315203.0
35	820935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9996893054897	21.470949387864273	13.101379618710293	25.42798168793573
2	11.825988999708244	21.768529224252507	45.41436143198297	20.991120344056277
3	17.376572663131824	27.978934131340203	30.244163359084858	24.400329846443107
4	10.020422966111655	17.86800172352545	40.67344460608123	31.438130704281665
5	10.414888214127487	41.24873031995937	35.65167003447046	12.68471143144268
6	29.47508185384092	40.05401543066466	18.824424861956064	11.646477853538356
7	26.98286737750931	32.77134081275951	23.028208584681135	17.217583225050046
8	23.77111403775369	41.01678316955391	20.092594468645526	15.11950832404687
9	27.082306188801887	14.554902535521752	21.367420348740616	36.99537092693575
10	14.95814755132446	29.50452427874258	34.2732180798256	21.26411009010737
11	33.82220742354687	21.5669455826742	27.571418054549028	17.0394289392299
12	25.35502245250665	24.902865640877405	32.78315856014901	16.958953346466927
13	29.658340103857476	23.193350636159124	26.408893733500012	20.739415526483388
14	19.46809897284305	25.211438728862035	27.493146771536765	27.827315526758152
15	20.357887442940413	37.449795331767845	22.786891778084392	19.405425447207346
16	19.203627500440675	28.681782602558677	32.41261331717869	19.701976579821963
17	18.963941786560177	31.889140798641712	29.589062929487557	19.557854485310557
18	19.70798729510383	26.91881954954754	35.55972928818949	17.81346386715914
19	23.847208968257345	26.225020919315266	28.55488222037173	21.372887892055658
20	24.567009007293226	27.65611144541494	29.523620422654588	18.253259124637246
21	22.343359767026904	26.827092307214357	27.014032684850132	23.815515240908606
22	23.330033065709994	31.391150600261504	27.47688912181364	17.801927212214864
23	18.629975639719078	31.077626699462208	30.38295009958202	19.909447561236696
24	22.913319407383995	27.360843499208816	31.120377067702943	18.60546002570425
25	20.378189568889017	29.50078544674563	30.689491222311823	19.431533762053537
26	17.716696562678973	31.333879926088414	31.655959932139655	19.29346357909296
27	19.72710001897765	30.453525352048942	29.65430282794024	20.165071801033168
28	18.35739983210519	30.871168759948915	28.724735579179693	22.046695828766204
29	20.42765226188619	28.859402615944337	28.29615131874418	22.416793803425296
30	18.568315155984816	28.920522810664494	30.930195334739434	21.580966698611252
31	22.633382161952984	27.378673036674144	29.199649622574352	20.788295178798517
32	19.84724678848833	30.202628641125855	29.506803927090697	20.443320643295117
33	18.055715953856616	30.780670350087018	31.932161311389496	19.231452384666866
34	20.33418226244674	30.042365412318183	31.499052130460885	18.12440019477419
35	19.845288240812216	30.74980634015651	30.726438840296645	18.67846657873463
36	18.086715036040655	29.7253508332945	32.358584415768604	19.829349714896242
37	19.485658365801324	29.599193348501945	28.275755408461873	22.639392877234854
38	19.685942867022447	31.828895525452396	28.552981523429004	19.932180084096153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	69.0
2	96.0
3	96.0
4	407.0
5	718.0
6	718.0
7	966.0
8	1214.0
9	1252.0
10	1290.0
11	1290.0
12	1862.0
13	2434.0
14	3577.5
15	4721.0
16	4721.0
17	7344.0
18	9967.0
19	9967.0
20	12863.5
21	15760.0
22	19033.5
23	22307.0
24	22307.0
25	30205.0
26	38103.0
27	38103.0
28	51045.0
29	63987.0
30	85869.5
31	107752.0
32	107752.0
33	133582.5
34	159413.0
35	159413.0
36	168997.5
37	178582.0
38	180553.0
39	182524.0
40	182524.0
41	163399.5
42	144275.0
43	128955.0
44	113635.0
45	113635.0
46	101471.0
47	89307.0
48	89307.0
49	83021.5
50	76736.0
51	70111.0
52	63486.0
53	63486.0
54	55898.0
55	48310.0
56	48310.0
57	45317.5
58	42325.0
59	35934.0
60	29543.0
61	29543.0
62	29568.0
63	29593.0
64	23755.0
65	17917.0
66	17917.0
67	15717.5
68	13518.0
69	13518.0
70	11026.5
71	8535.0
72	6762.0
73	4989.0
74	4989.0
75	3677.5
76	2366.0
77	2366.0
78	2565.0
79	2764.0
80	2363.0
81	1962.0
82	1962.0
83	1814.5
84	1667.0
85	1667.0
86	1085.5
87	504.0
88	372.0
89	240.0
90	240.0
91	149.0
92	58.0
93	42.0
94	26.0
95	26.0
96	17.0
97	8.0
98	8.0
99	11.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009117377112952145
2	6.753612676260848E-5
3	0.0
4	2.0260838028782544E-4
5	6.753612676260848E-5
6	6.753612676260848E-5
7	3.3768063381304245E-4
8	0.002633908943741731
9	0.003174197957842599
10	7.428973943886933E-4
11	0.0051327456339582455
12	5.402890141008678E-4
13	4.0521676057565087E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0260838028782544E-4
22	6.753612676260848E-5
23	0.0
24	0.0
25	2.0260838028782544E-4
26	6.753612676260848E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.0260838028782544E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1480689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.01628105158652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10914450405366	46.554651964959874
2	11.200029736679854	12.547680270319702
3	2.591642524074692	4.35522528041433
4	0.9599219022484652	2.150850202556943
5	0.49739352302013995	1.3931067689367462
6	0.286831818518992	0.9640351056418507
7	0.19840058966365154	0.7779564233979717
8	0.13978239321714794	0.6264071859612111
9	0.10741681031389153	0.5415381212587115
>10	0.7208619641708296	7.921359799955773
>50	0.09238169347584221	3.639386568555033
>100	0.08179396639516852	9.381134512815468
>500	0.009558717304544738	3.620955608273975
>1k	0.004234874755178049	3.6769399848169946
>5k	6.049821078825785E-4	1.8487722021355422
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	6514	0.43993032973163165	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5368	0.36253392846168236	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	5281	0.3566582854333354	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	5108	0.3449745355034041	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	5006	0.33808585057361806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3686	0.2489381632469749	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2901	0.19592230373832722	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2764	0.18666985437184985	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2638	0.1781603023997612	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	2555	0.1725548038784647	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2086	0.14088036042680133	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	1724	0.11643228253873704	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1709	0.11541924063729792	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	1613	0.1089357724680875	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1587	0.10717983317225968	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1560	0.10535635774966924	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1552	0.10481606873556837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.753612676260849E-5	0.0
8	0.0	0.0	0.0	6.753612676260849E-5	0.0
9	0.0	0.0	0.0	1.3507225352521698E-4	0.0
10	0.0	0.0	0.0	2.0260838028782546E-4	0.0
11	0.0	0.0	0.0	2.0260838028782546E-4	0.0
12	0.0	0.0	0.0	2.0260838028782546E-4	0.0
13	0.0	0.0	0.0	2.0260838028782546E-4	0.0
14	0.0	0.0	0.0	2.7014450705043397E-4	0.0
15	0.0	0.0	0.0	2.7014450705043397E-4	0.0
16	0.0	0.0	0.0	2.7014450705043397E-4	0.0
17	0.0	0.0	0.0	2.7014450705043397E-4	0.0
18	0.0	0.0	0.0	4.727528873382594E-4	0.0
19	0.0	0.0	0.0	5.402890141008679E-4	0.0
20	0.0	0.0	6.753612676260849E-5	8.779696479139104E-4	0.0
21	0.0	0.0	6.753612676260849E-5	0.0010805780282017359	0.0
22	0.0	0.0	6.753612676260849E-5	0.0012831864084895614	0.0
23	0.0	0.0	6.753612676260849E-5	0.0014857947887773866	0.0
24	0.0	0.0	6.753612676260849E-5	0.0024313005634539054	0.0
25	0.0	0.0	6.753612676260849E-5	0.0029715895775547733	0.0
26	0.0	0.0	6.753612676260849E-5	0.0035794147184182497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	255	0.0	20.704594	29
GCGCAAG	275	0.0	20.364431	1
TAGCGGC	260	0.0	20.306427	30
AAGACGG	280	0.0	20.004158	5
GCGGTAC	40	0.0044856956	19.998756	26
AGCGGCG	265	0.0	19.923288	31
CAAGACG	285	0.0	19.65188	4
TAGGACC	2070	0.0	19.5583	4
TCTAGCG	275	0.0	19.198805	28
CCTCGAC	75	1.5100668E-6	19.198803	30
GCGGCGC	280	0.0	18.855968	32
GGCATCG	255	0.0	18.822357	26
AGGACCT	2905	0.0	18.730228	5
ACCGTCG	240	0.0	18.670547	8
CCGTCGT	240	0.0	18.668655	9
CGCAAGA	300	0.0	18.667395	2
GTCGTAG	240	0.0	18.666765	11
TACCGTC	250	0.0	18.56386	7
AGGCCCG	350	0.0	18.28581	10
GGACCTG	2875	0.0	18.202045	6
>>END_MODULE