FastQCFastQC Report
Thu 2 Feb 2017
SRR4062967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593181
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT142310.5487854492224029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT97910.37756716557772096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT57130.22030857082479008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.16188611593251687No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA38140.14707804815784165No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA33710.12999478246987003No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG31390.12104824152267044No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28880.11136900972203638No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC27270.10516041880609182No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26780.10327084765776087No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG26330.10153552721541613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG652.105502E-417.2266795
TACCGTC5400.016.5918527
ACCGTCG5450.016.1460698
GTATAGG2050.015.6268421
TAGGACG3400.015.5254264
CGTCGTA5750.015.30425510
CGTTTAT6600.015.27204631
ATACCGT6200.015.2221926
CCGTCGT5800.015.172039
ATCGTTT6900.014.60832629
CATCGTT6800.014.58730228
CGTGAAA2600.014.1534889
CGTACAT1604.9094524E-913.99937626
CGGCATC7100.013.97174625
CTTACAC2900.013.79062753
CTAGCGG5500.013.67238129
TCTAGCG5800.013.51637828
CCGTCTA952.4293325E-413.4733439
TCTAGAT6300.013.465432
TCGTTTA7400.013.40480730
Produced by FastQC (version 0.11.5)