##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062967_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2593181
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.143170877775212	32.0	32.0	32.0	32.0	32.0
2	30.706265008111657	32.0	32.0	32.0	32.0	32.0
3	30.772695388405207	32.0	32.0	32.0	32.0	32.0
4	30.841452254971788	32.0	32.0	32.0	32.0	32.0
5	30.750241113134795	32.0	32.0	32.0	32.0	32.0
6	34.39615360439553	36.0	36.0	36.0	32.0	36.0
7	34.29877281994585	36.0	36.0	36.0	32.0	36.0
8	34.28182606613267	36.0	36.0	36.0	32.0	36.0
9	34.43195403637463	36.0	36.0	36.0	32.0	36.0
10	34.12550223065802	36.0	36.0	36.0	32.0	36.0
11	34.46013139846389	36.0	36.0	36.0	32.0	36.0
12	34.24512133938973	36.0	36.0	36.0	32.0	36.0
13	34.34291860074557	36.0	36.0	36.0	32.0	36.0
14	34.24136456344544	36.0	36.0	36.0	32.0	36.0
15	34.184954694639515	36.0	36.0	36.0	32.0	36.0
16	34.19414765108953	36.0	36.0	36.0	32.0	36.0
17	34.11895390256214	36.0	36.0	36.0	32.0	36.0
18	34.15270742767281	36.0	36.0	36.0	32.0	36.0
19	34.13896638915679	36.0	36.0	36.0	32.0	36.0
20	34.10515502003138	36.0	36.0	36.0	32.0	36.0
21	34.088658678279685	36.0	36.0	36.0	32.0	36.0
22	34.061356688946894	36.0	36.0	36.0	32.0	36.0
23	34.020086141306756	36.0	36.0	36.0	32.0	36.0
24	34.00231028994891	36.0	36.0	36.0	32.0	36.0
25	33.9797734905508	36.0	36.0	36.0	32.0	36.0
26	33.93948937617544	36.0	36.0	36.0	32.0	36.0
27	33.94571956219022	36.0	36.0	36.0	32.0	36.0
28	33.93080429017488	36.0	36.0	36.0	32.0	36.0
29	33.89735309644795	36.0	36.0	36.0	32.0	36.0
30	33.8609985188076	36.0	36.0	36.0	32.0	36.0
31	33.87255883796773	36.0	36.0	36.0	32.0	36.0
32	33.8251649229267	36.0	36.0	36.0	32.0	36.0
33	33.77519810610983	36.0	36.0	36.0	32.0	36.0
34	33.76380900523334	36.0	36.0	36.0	32.0	36.0
35	33.725678230713555	36.0	36.0	36.0	27.0	36.0
36	33.67447663699526	36.0	36.0	36.0	27.0	36.0
37	33.663324310952454	36.0	36.0	36.0	27.0	36.0
38	33.04392288852957	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	5.0
9	10.0
10	27.0
11	28.0
12	10.0
13	15.0
14	704.0
15	1342.0
16	1540.0
17	1825.0
18	2186.0
19	2662.0
20	3465.0
21	4439.0
22	6399.0
23	9173.0
24	13610.0
25	19794.0
26	28443.0
27	39528.0
28	54738.0
29	72947.0
30	97108.0
31	128879.0
32	173573.0
33	258517.0
34	557158.0
35	1115052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23558500505396	18.55079021762548	12.544062923978958	24.6695618533416
2	15.620640055313423	21.13118571975356	38.4961509019298	24.752023323003222
3	17.640497842351465	26.36353784167268	29.66096628869411	26.33499802728175
4	12.077013375455245	16.587546681434016	37.41151544787182	33.923924495238914
5	13.828630352409283	37.52700354545658	33.708561463964294	14.935804638169845
6	31.478361468084493	37.22001021913292	17.91016803725163	13.391460275530962
7	27.970781831272095	32.07037996962032	22.319922905497148	17.638915293610435
8	27.43355153431604	32.691237575558915	20.709747703105265	19.165463187019775
9	27.786677196860378	14.381928274881245	19.94074452274391	37.89065000551447
10	16.106072449522227	27.276874529292517	31.87144233835717	24.74561068282809
11	35.240779982530796	22.508160971179674	23.35416397526546	18.89689507102407
12	24.19408641274021	25.209815384057695	29.36142775112384	21.234670452078255
13	28.840010290141095	20.620547961583988	26.06009087567635	24.479350872598566
14	23.574071752943105	20.755787382974823	26.546120693811044	29.124020170271024
15	24.274247362635244	27.597886250988406	24.18965883010935	23.938207556266995
16	24.244793213378554	26.79062473725985	25.106195229525934	23.858386819835655
17	22.72444826898577	26.69384130990626	26.418212050930855	24.163498370177113
18	23.68936097513757	25.418767009547523	27.95371744781875	22.93815456749616
19	24.537873525960112	25.923780514848094	27.17108137845604	22.367264580735753
20	25.00074237446448	24.661062671445116	26.858915300279868	23.479279653810543
21	26.385968105755207	24.810808455202782	25.805638010232908	22.9975854288091
22	24.87883664669356	25.075251052164937	26.310698819546722	23.735213481594776
23	23.340018203415454	25.10170001388396	26.947688320503527	24.61059346219706
24	23.572443206471537	26.272259503256944	26.77438676565479	23.380910524616734
25	23.877525832044462	25.325123133709514	26.845270007058254	23.95208102718777
26	23.418009331703608	26.190366907898316	27.392372410340393	22.99925135005768
27	24.21801543721948	25.849745116959266	26.235714170016127	23.696525275805126
28	23.16674116155724	25.76425394491903	27.356991985018237	23.712012908505493
29	23.30714121529165	26.052184202041584	27.127007218194972	23.513667364471793
30	23.497090745299843	25.980022397867575	27.36176412515657	23.161122731676016
31	23.74630836144082	25.972938139360174	26.246981321407993	24.03377217779101
32	23.22973193422402	26.181158920054624	26.246296254159784	24.34281289156157
33	23.15460442586408	25.651576974399713	26.744544802425047	24.44927379731116
34	23.704840164051394	25.87238115954438	27.08352135067027	23.33925732573396
35	24.568737759079397	25.76885902084977	26.68426196553605	22.97814125453478
36	23.444895616703796	26.524480551938634	26.25291582071868	23.77770801063889
37	24.293538877344652	26.41029972340107	26.154835236204377	23.1413261630499
38	23.326952104532857	26.39221726618594	26.620830829819415	23.659999799461787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2146.0
1	1807.5
2	1469.0
3	1469.0
4	3113.0
5	4757.0
6	4757.0
7	5304.5
8	5852.0
9	5544.0
10	5236.0
11	5236.0
12	6728.5
13	8221.0
14	10393.5
15	12566.0
16	12566.0
17	18296.0
18	24026.0
19	24026.0
20	28370.5
21	32715.0
22	33727.0
23	34739.0
24	34739.0
25	37860.0
26	40981.0
27	40981.0
28	50696.5
29	60412.0
30	70451.0
31	80490.0
32	80490.0
33	94435.5
34	108381.0
35	108381.0
36	117307.5
37	126234.0
38	140226.5
39	154219.0
40	154219.0
41	163427.5
42	172636.0
43	190126.5
44	207617.0
45	207617.0
46	213189.5
47	218762.0
48	218762.0
49	225335.5
50	231909.0
51	225760.0
52	219611.0
53	219611.0
54	207120.0
55	194629.0
56	194629.0
57	186901.0
58	179173.0
59	158500.5
60	137828.0
61	137828.0
62	129929.5
63	122031.0
64	99602.0
65	77173.0
66	77173.0
67	64102.5
68	51032.0
69	51032.0
70	40525.0
71	30018.0
72	23378.0
73	16738.0
74	16738.0
75	12479.5
76	8221.0
77	8221.0
78	8056.0
79	7891.0
80	6316.5
81	4742.0
82	4742.0
83	4853.5
84	4965.0
85	4965.0
86	3301.0
87	1637.0
88	1458.0
89	1279.0
90	1279.0
91	970.0
92	661.0
93	580.5
94	500.0
95	500.0
96	479.5
97	459.0
98	459.0
99	842.0
100	1225.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12089399081668421
2	0.05418056047765274
3	0.012031555066923598
4	0.002815075384248149
5	1.1568802948964996E-4
6	2.3137605897929992E-4
7	0.0
8	2.3137605897929992E-4
9	1.1568802948964996E-4
10	3.8562676496549993E-4
11	0.0017738831188412994
12	0.0026222620017653992
13	0.015733572010592396
14	0.009872045183116797
15	0.024333048869323044
16	0.012147243096413247
17	0.021903600250040393
18	0.005861526827475599
19	0.008560914182234099
20	0.005784401474482499
21	0.006709905710399699
22	0.007481159240330697
23	0.010064858565599548
24	0.015887822716578594
25	0.018163020629875043
26	0.019821215719226695
27	0.008753727564716847
28	0.006247153592441099
29	0.011144613507502947
30	0.0029693260902343495
31	0.0061314655629514485
32	0.007635409946316897
33	0.009293605035668548
34	0.012918496626344245
35	0.017083265687971645
36	0.016543388217019946
37	0.009910607859613348
38	0.0061314655629514485
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2593181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.56717442336829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54488629341306	38.436809045458666
2	13.191597956669243	13.077404736823464
3	4.20536836920657	6.253446824129338
4	1.7722073989699285	3.513732530365052
5	0.8930846417557701	2.2133841106369823
6	0.5241917691229493	1.5589622910846737
7	0.33644323359374645	1.167357831017229
8	0.23215871130657012	0.9205961069789733
9	0.18302822791183504	0.8164972887575341
>10	0.9284688816186465	8.253468171266261
>50	0.07929529622708105	2.80924477549707
>100	0.08764590257103817	9.25078313863689
>500	0.014363503410188265	4.767141077761463
>1k	0.007025626668026048	5.810624276912511
>5k	1.5612503706724553E-4	0.5999022367118257
>10k+	7.806251853362276E-5	0.5506455579622028
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	14231	0.5487854492224029	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	9791	0.37756716557772096	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5713	0.22030857082479008	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4198	0.16188611593251687	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3814	0.14707804815784165	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3371	0.12999478246987003	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3139	0.12104824152267044	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2888	0.11136900972203638	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2727	0.10516041880609182	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2678	0.10327084765776087	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2633	0.10153552721541613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.712535299309998E-5	0.0
3	0.0	0.0	0.0	7.712535299309998E-5	0.0
4	0.0	0.0	0.0	1.9281338248274994E-4	0.0
5	0.0	0.0	0.0	2.3137605897929995E-4	0.0
6	7.712535299309998E-5	0.0	0.0	2.3137605897929995E-4	0.0
7	7.712535299309998E-5	0.0	0.0	2.3137605897929995E-4	0.0
8	7.712535299309998E-5	0.0	0.0	2.3137605897929995E-4	0.0
9	7.712535299309998E-5	0.0	0.0	2.3137605897929995E-4	3.856267649654999E-5
10	7.712535299309998E-5	0.0	0.0	2.6993873547584993E-4	3.856267649654999E-5
11	7.712535299309998E-5	0.0	0.0	2.6993873547584993E-4	3.856267649654999E-5
12	7.712535299309998E-5	0.0	0.0	2.6993873547584993E-4	7.712535299309998E-5
13	7.712535299309998E-5	0.0	0.0	2.6993873547584993E-4	2.6993873547584993E-4
14	7.712535299309998E-5	0.0	0.0	3.470640884689499E-4	2.6993873547584993E-4
15	7.712535299309998E-5	0.0	0.0	3.470640884689499E-4	6.555655004413498E-4
16	1.1568802948964997E-4	0.0	0.0	3.470640884689499E-4	7.712535299309998E-4
17	1.1568802948964997E-4	0.0	0.0	3.470640884689499E-4	7.712535299309998E-4
18	1.1568802948964997E-4	0.0	0.0	3.470640884689499E-4	7.712535299309998E-4
19	1.1568802948964997E-4	0.0	0.0	3.856267649654999E-4	7.712535299309998E-4
20	1.1568802948964997E-4	0.0	0.0	5.398774709516999E-4	8.869415594206498E-4
21	1.1568802948964997E-4	0.0	0.0	8.098162064275498E-4	9.640669124137498E-4
22	1.5425070598619996E-4	0.0	0.0	0.0011183176183999497	9.640669124137498E-4
23	1.5425070598619996E-4	0.0	0.0	0.0016581950893516497	0.0010026295889102998
24	1.5425070598619996E-4	0.0	0.0	0.0032392648257101994	0.0010026295889102998
25	1.5425070598619996E-4	0.0	0.0	0.0041262063851308485	0.0010026295889102998
26	1.5425070598619996E-4	0.0	0.0	0.005591588091999749	0.0010026295889102998
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	65	2.105502E-4	17.226679	5
TACCGTC	540	0.0	16.591852	7
ACCGTCG	545	0.0	16.146069	8
GTATAGG	205	0.0	15.626842	1
TAGGACG	340	0.0	15.525426	4
CGTCGTA	575	0.0	15.304255	10
CGTTTAT	660	0.0	15.272046	31
ATACCGT	620	0.0	15.222192	6
CCGTCGT	580	0.0	15.17203	9
ATCGTTT	690	0.0	14.608326	29
CATCGTT	680	0.0	14.587302	28
CGTGAAA	260	0.0	14.153488	9
CGTACAT	160	4.9094524E-9	13.999376	26
CGGCATC	710	0.0	13.971746	25
CTTACAC	290	0.0	13.7906275	3
CTAGCGG	550	0.0	13.672381	29
TCTAGCG	580	0.0	13.516378	28
CCGTCTA	95	2.4293325E-4	13.473343	9
TCTAGAT	630	0.0	13.46543	2
TCGTTTA	740	0.0	13.404807	30
>>END_MODULE