FastQCFastQC Report
Thu 2 Feb 2017
SRR4062967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593181
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT177110.6829835634303969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT132970.5127679093746252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT79040.3047993950287311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55870.2154496735862248No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG34180.13180722826520785No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC32570.12559863734926333No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA32530.12544438664327712No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA28950.11163894845751222No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28860.11129188436904328No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28200.10874674772027099No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA27200.10489048007061598No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA27160.10473622936462976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG5450.016.150428
CCGTCGT5750.015.5852119
CTAGCGG5350.015.550429
TCTAGCG5450.015.26507128
TACCGTC6000.015.2034177
ATCGTTT5950.015.05785329
AACCGCG5250.014.936697
CGTCGTA6000.014.93467410
GAACCGC5600.014.859916
TAGCGGC5600.014.85618630
GTCGTAG6150.014.57013211
CATCGTT6400.014.49906528
AGAACCG6000.014.4024045
TAGCGTA5900.014.3761127
ATACCGT6350.014.3648796
AATAGCG6050.014.0188685
CGCGGTC5400.013.92717610
ATAGCGT6100.013.9042286
AGCGTAT6000.013.8697848
GTATTAG5550.013.837481
Produced by FastQC (version 0.11.5)