##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2593181
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.076252679623984	32.0	32.0	32.0	32.0	32.0
2	31.29588061920861	32.0	32.0	32.0	32.0	32.0
3	31.393642017275308	32.0	32.0	32.0	32.0	32.0
4	31.50099626674729	32.0	32.0	32.0	32.0	32.0
5	31.42645654121328	32.0	32.0	32.0	32.0	32.0
6	34.93862441534162	36.0	36.0	36.0	36.0	36.0
7	34.96736672064156	36.0	36.0	36.0	36.0	36.0
8	34.92469326283048	36.0	36.0	36.0	36.0	36.0
9	35.00743951154971	36.0	36.0	36.0	36.0	36.0
10	34.87197461341881	36.0	36.0	36.0	32.0	36.0
11	35.04313235366139	36.0	36.0	36.0	36.0	36.0
12	34.94292415377099	36.0	36.0	36.0	36.0	36.0
13	34.99173987469444	36.0	36.0	36.0	36.0	36.0
14	34.93928769337736	36.0	36.0	36.0	32.0	36.0
15	34.916630964055344	36.0	36.0	36.0	32.0	36.0
16	34.91862002690904	36.0	36.0	36.0	32.0	36.0
17	34.89006629309716	36.0	36.0	36.0	32.0	36.0
18	34.88477356574801	36.0	36.0	36.0	32.0	36.0
19	34.876511897935394	36.0	36.0	36.0	32.0	36.0
20	34.8598173440265	36.0	36.0	36.0	32.0	36.0
21	34.84537793543914	36.0	36.0	36.0	32.0	36.0
22	34.83380296246193	36.0	36.0	36.0	32.0	36.0
23	34.779418405425616	36.0	36.0	36.0	32.0	36.0
24	34.76407431644764	36.0	36.0	36.0	32.0	36.0
25	34.740808296836974	36.0	36.0	36.0	32.0	36.0
26	34.67656249216696	36.0	36.0	36.0	32.0	36.0
27	34.66351558182788	36.0	36.0	36.0	32.0	36.0
28	34.63726056916197	36.0	36.0	36.0	32.0	36.0
29	34.595582799658025	36.0	36.0	36.0	32.0	36.0
30	34.562319406165635	36.0	36.0	36.0	32.0	36.0
31	34.55882292828769	36.0	36.0	36.0	32.0	36.0
32	34.52276605450989	36.0	36.0	36.0	32.0	36.0
33	34.46892060369099	36.0	36.0	36.0	32.0	36.0
34	34.44825178034237	36.0	36.0	36.0	32.0	36.0
35	34.39781411324547	36.0	36.0	36.0	32.0	36.0
36	34.36560926522291	36.0	36.0	36.0	32.0	36.0
37	34.34302002058476	36.0	36.0	36.0	32.0	36.0
38	33.91643853629962	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	23.0
21	62.0
22	243.0
23	700.0
24	1893.0
25	4506.0
26	9363.0
27	18188.0
28	31020.0
29	48773.0
30	72375.0
31	102549.0
32	146365.0
33	231430.0
34	559675.0
35	1366009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.659827625304864	19.741329972433007	13.292589615866987	25.306252786395138
2	14.763797822057157	22.069805385740523	37.7621924578346	25.404204334367712
3	16.77241195273296	26.966455484595947	30.09041790758146	26.17071465508964
4	11.630400998311725	17.37651638260081	37.420647560637946	33.57243505844952
5	13.287662252523926	37.897446378577655	33.64756785105546	15.167323517842956
6	31.454312605734202	37.6233862941095	17.547278878379686	13.375022221776609
7	27.513941436134708	32.26307271141989	22.47061414045903	17.752371711986374
8	27.1582880853952	32.9447923736657	20.683385265625965	19.213534275313133
9	27.573741233310223	14.190410226705652	19.911000696476048	38.32484784350808
10	15.825722921626834	27.458450275687017	32.02870785351298	24.68711894917317
11	35.255017730052	22.366976404279892	23.395994300159465	18.98201156550864
12	23.896969505324364	25.260318050389213	29.523433444882603	21.31927899940382
13	28.763460348947156	20.556098862705596	26.216540977613516	24.46389981073373
14	23.356641900430397	20.662961821793388	26.73145453402597	29.248941743750244
15	24.18720482681309	27.553186607490954	24.314345971222217	23.94526259447374
16	24.182847244368983	26.641642060465504	25.182430381836053	23.99308031332946
17	22.63910617885909	26.582062725278337	26.648313403499408	24.130517692363163
18	23.628470207054576	25.054556546573494	28.34537967075958	22.971593575612346
19	24.451744787579425	25.63866540746674	27.5974179974325	22.31217180752134
20	24.81731896076672	24.442682558602733	27.29813306514277	23.441865415487772
21	26.121847401142535	24.666027553835487	26.270699504623284	22.94142554039869
22	24.640084899588572	24.936747569876534	26.7669321964028	23.656235334132095
23	23.187838533010126	24.676361863878014	27.458242293035482	24.677557310076377
24	23.422198450474532	25.880723327835582	27.28641001148782	23.410668210202065
25	23.70838121534178	24.971347920314056	27.39987197186466	23.920398892479504
26	23.366591070966507	25.725855619025435	27.833537265620873	23.074016044387182
27	24.12504044266936	25.465635396272603	26.750931386480755	23.658392774577287
28	23.091562062193113	25.30552244521304	27.83245751067897	23.770457981914873
29	23.275043276963697	25.494826624134603	27.628923704130177	23.601206394771516
30	23.435464011189346	25.43027270367938	27.860955328609922	23.273307956521354
31	23.712575404493556	25.369613613550307	26.82076569279198	24.097045289164157
32	23.268718998018265	25.693540096121325	26.747226668713058	24.29051423714735
33	23.0917163128991	25.190682794606317	27.355167263681167	24.362433628813417
34	23.659474598957804	25.35376435351023	27.66501836933095	23.321742678201023
35	24.61004457459776	25.19099129601829	27.19972111472358	22.999243014660372
36	23.523464038954472	25.93579082987266	26.82716709709041	23.713578034082463
37	24.34619874200837	25.803328036107004	26.65826257403552	23.19221064784911
38	23.28353604454762	25.76095758875203	27.18303395830602	23.772472408394325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1073.0
1	932.5
2	792.0
3	792.0
4	2827.0
5	4862.0
6	4862.0
7	5629.5
8	6397.0
9	6100.5
10	5804.0
11	5804.0
12	7494.0
13	9184.0
14	11455.0
15	13726.0
16	13726.0
17	20721.5
18	27717.0
19	27717.0
20	31505.5
21	35294.0
22	35542.5
23	35791.0
24	35791.0
25	38392.0
26	40993.0
27	40993.0
28	50254.0
29	59515.0
30	68840.0
31	78165.0
32	78165.0
33	93458.5
34	108752.0
35	108752.0
36	117548.0
37	126344.0
38	139641.0
39	152938.0
40	152938.0
41	162317.5
42	171697.0
43	190123.5
44	208550.0
45	208550.0
46	213827.0
47	219104.0
48	219104.0
49	226928.0
50	234752.0
51	227372.5
52	219993.0
53	219993.0
54	207294.5
55	194596.0
56	194596.0
57	186463.0
58	178330.0
59	158088.0
60	137846.0
61	137846.0
62	129712.5
63	121579.0
64	99144.5
65	76710.0
66	76710.0
67	63582.0
68	50454.0
69	50454.0
70	40138.5
71	29823.0
72	23139.0
73	16455.0
74	16455.0
75	11954.5
76	7454.0
77	7454.0
78	7578.5
79	7703.0
80	6003.0
81	4303.0
82	4303.0
83	4252.0
84	4201.0
85	4201.0
86	2648.0
87	1095.0
88	938.0
89	781.0
90	781.0
91	486.0
92	191.0
93	147.5
94	104.0
95	104.0
96	94.0
97	84.0
98	84.0
99	56.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008676602211723748
2	0.0
3	0.0
4	1.1568802948964996E-4
5	3.856267649654999E-5
6	1.5425070598619996E-4
7	4.6275211795859984E-4
8	0.0023523232662895494
9	0.004897459915061848
10	8.869415594206497E-4
11	0.004858897238565299
12	2.6993873547584993E-4
13	3.470640884689499E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1568802948964996E-4
22	0.0
23	2.6993873547584993E-4
24	0.0
25	3.856267649654999E-5
26	0.0
27	1.5425070598619996E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.856267649654999E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2593181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.941466571094956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29134485975943	35.3428614782718
2	14.609247411360545	13.715589979784735
3	4.522336968449921	6.368553888831567
4	1.955627872398607	3.6720016159080306
5	0.9768529874546494	2.2927455927738336
6	0.5849645292797977	1.6475455737878353
7	0.38396982194606394	1.2616874592833311
8	0.2614269556466841	0.9817411759417558
9	0.1880072067586814	0.7942800610068822
>10	1.0227947198959142	8.693903709510677
>50	0.08350229217778542	2.7423491672687117
>100	0.09616477267004811	9.689751986102326
>500	0.015509740326197799	4.917241664693745
>1k	0.00791986740061137	6.156475634137958
>5k	1.6499723751273686E-4	0.5224532963054265
>10k+	1.6499723751273686E-4	1.2008177163915696
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	17711	0.6829835634303969	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	13297	0.5127679093746252	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	7904	0.3047993950287311	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5587	0.2154496735862248	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3418	0.13180722826520785	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3257	0.12559863734926333	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3253	0.12544438664327712	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2895	0.11163894845751222	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2886	0.11129188436904328	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2820	0.10874674772027099	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2720	0.10489048007061598	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2716	0.10473622936462976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.856267649654999E-5
2	0.0	0.0	0.0	0.0	3.856267649654999E-5
3	0.0	0.0	0.0	0.0	3.856267649654999E-5
4	0.0	0.0	0.0	0.0	3.856267649654999E-5
5	0.0	0.0	0.0	3.856267649654999E-5	3.856267649654999E-5
6	0.0	0.0	0.0	3.856267649654999E-5	7.712535299309998E-5
7	0.0	0.0	0.0	3.856267649654999E-5	1.1568802948964997E-4
8	0.0	0.0	0.0	3.856267649654999E-5	1.1568802948964997E-4
9	0.0	0.0	0.0	3.856267649654999E-5	1.1568802948964997E-4
10	0.0	0.0	7.712535299309998E-5	7.712535299309998E-5	1.1568802948964997E-4
11	0.0	0.0	7.712535299309998E-5	7.712535299309998E-5	1.1568802948964997E-4
12	0.0	0.0	7.712535299309998E-5	7.712535299309998E-5	3.856267649654999E-4
13	3.856267649654999E-5	0.0	7.712535299309998E-5	7.712535299309998E-5	4.627521179585999E-4
14	3.856267649654999E-5	0.0	7.712535299309998E-5	1.5425070598619996E-4	5.013147944551499E-4
15	3.856267649654999E-5	0.0	7.712535299309998E-5	1.5425070598619996E-4	7.326908534344498E-4
16	1.1568802948964997E-4	0.0	7.712535299309998E-5	1.9281338248274994E-4	7.326908534344498E-4
17	1.1568802948964997E-4	0.0	7.712535299309998E-5	1.9281338248274994E-4	7.326908534344498E-4
18	1.1568802948964997E-4	0.0	7.712535299309998E-5	1.9281338248274994E-4	7.712535299309998E-4
19	1.1568802948964997E-4	0.0	1.1568802948964997E-4	2.3137605897929995E-4	7.712535299309998E-4
20	1.1568802948964997E-4	0.0	1.1568802948964997E-4	3.856267649654999E-4	9.255042359171998E-4
21	1.1568802948964997E-4	0.0	1.1568802948964997E-4	6.555655004413498E-4	9.255042359171998E-4
22	1.1568802948964997E-4	0.0	1.1568802948964997E-4	0.0010026295889102998	9.640669124137498E-4
23	1.1568802948964997E-4	0.0	1.1568802948964997E-4	0.0016196324128550997	0.0010026295889102998
24	1.1568802948964997E-4	0.0	1.1568802948964997E-4	0.003162139472717099	0.0010411922654068497
25	1.1568802948964997E-4	0.0	1.1568802948964997E-4	0.003971955679144649	0.0010411922654068497
26	1.1568802948964997E-4	0.0	1.1568802948964997E-4	0.005514462739006649	0.0010797549419033997
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	545	0.0	16.15042	8
CCGTCGT	575	0.0	15.585211	9
CTAGCGG	535	0.0	15.5504	29
TCTAGCG	545	0.0	15.265071	28
TACCGTC	600	0.0	15.203417	7
ATCGTTT	595	0.0	15.057853	29
AACCGCG	525	0.0	14.93669	7
CGTCGTA	600	0.0	14.934674	10
GAACCGC	560	0.0	14.85991	6
TAGCGGC	560	0.0	14.856186	30
GTCGTAG	615	0.0	14.570132	11
CATCGTT	640	0.0	14.499065	28
AGAACCG	600	0.0	14.402404	5
TAGCGTA	590	0.0	14.376112	7
ATACCGT	635	0.0	14.364879	6
AATAGCG	605	0.0	14.018868	5
CGCGGTC	540	0.0	13.927176	10
ATAGCGT	610	0.0	13.904228	6
AGCGTAT	600	0.0	13.869784	8
GTATTAG	555	0.0	13.83748	1
>>END_MODULE