FastQCFastQC Report
Thu 2 Feb 2017
SRR4062966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744369
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37060.49787135143994443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26350.3539910985008779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14950.20084124943408446No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13480.18109297942284003No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13120.17625666839967813No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12860.17276377710517232No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12540.16846483397347284No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11920.16013563165580513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.1578518181170898No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11550.1551649786597776No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11530.1548962947140464No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11500.15449326879544956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11460.15395590090398714No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10720.1440145949119321No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10550.1417307813732168No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10510.14119341348175435No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10320.1386409159973078No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10050.13501368272993636No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10040.13487934075707075No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9530.12802790014092474No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9470.12722184830373107No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9430.12668448041226865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9370.12587842857507497No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9230.1239976409549565No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8790.11808659414886973No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8530.11459370285436389No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8490.11405633496290146No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8450.11351896707143902No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT8400.11284725720711099No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG8060.10827963012968031No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8040.10801094618394907No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7910.10626450053669617No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7890.10599581659096496No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7610.10223424135072792No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7500.10075647964920625No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG7500.10075647964920625No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT7470.10035345373060942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG400.00446033420.0167681
CTATACT507.194407E-419.1993144
GTATTGA802.7712267E-618.015091
GTGTTAG450.0088048117.7926831
TGTTAGG450.00883213217.7831172
AGGCCCG3300.016.97023210
GTCCTAG854.9395967E-616.955381
CTAGCGG2300.016.69505729
CGAGCCG3450.016.23239515
TCTAGAC703.6914367E-416.001583
TAGCGGC2300.015.9994330
AACGAAT600.002441402315.99942931
GAGCCGC3850.015.37607516
GATATAC1255.075526E-815.3728781
TCTAGCG2400.015.33175628
TCTAGAT2200.015.2773142
ATTATTA951.4315261E-515.15735421
ACCGTCG1901.8189894E-1215.1563378
CTTAAGA756.22649E-414.9378172
CCGAGCC3650.014.90457814
Produced by FastQC (version 0.11.5)