##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062966_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 744369 Sequences flagged as poor quality 0 Sequence length 38 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.134465567480646 32.0 32.0 32.0 32.0 32.0 2 30.753266189215296 32.0 32.0 32.0 32.0 32.0 3 30.82334567936064 32.0 32.0 32.0 32.0 32.0 4 30.878118245117676 32.0 32.0 32.0 32.0 32.0 5 30.82349748578998 32.0 32.0 32.0 32.0 32.0 6 34.4485275448064 36.0 36.0 36.0 32.0 36.0 7 34.32660683075195 36.0 36.0 36.0 32.0 36.0 8 34.29939855098748 36.0 36.0 36.0 32.0 36.0 9 34.41798758411487 36.0 36.0 36.0 32.0 36.0 10 34.166964234136564 36.0 36.0 36.0 32.0 36.0 11 34.46228819308703 36.0 36.0 36.0 32.0 36.0 12 34.25636478681944 36.0 36.0 36.0 32.0 36.0 13 34.36154111737593 36.0 36.0 36.0 32.0 36.0 14 34.246485278134905 36.0 36.0 36.0 32.0 36.0 15 34.206220302027624 36.0 36.0 36.0 32.0 36.0 16 34.22121017935997 36.0 36.0 36.0 32.0 36.0 17 34.12223373085123 36.0 36.0 36.0 32.0 36.0 18 34.1591603089328 36.0 36.0 36.0 32.0 36.0 19 34.143994443616 36.0 36.0 36.0 32.0 36.0 20 34.11100139849994 36.0 36.0 36.0 32.0 36.0 21 34.09053708577332 36.0 36.0 36.0 32.0 36.0 22 34.05145834928644 36.0 36.0 36.0 32.0 36.0 23 34.0281285222786 36.0 36.0 36.0 32.0 36.0 24 34.0034552755421 36.0 36.0 36.0 32.0 36.0 25 33.99356233266028 36.0 36.0 36.0 32.0 36.0 26 33.947078666629054 36.0 36.0 36.0 32.0 36.0 27 33.944758580757664 36.0 36.0 36.0 32.0 36.0 28 33.93585170795667 36.0 36.0 36.0 32.0 36.0 29 33.895894374967256 36.0 36.0 36.0 32.0 36.0 30 33.85911019937692 36.0 36.0 36.0 32.0 36.0 31 33.87516809539355 36.0 36.0 36.0 32.0 36.0 32 33.83154188312517 36.0 36.0 36.0 32.0 36.0 33 33.77251470708748 36.0 36.0 36.0 32.0 36.0 34 33.77544604893541 36.0 36.0 36.0 32.0 36.0 35 33.717218207636265 36.0 36.0 36.0 27.0 36.0 36 33.66231936042474 36.0 36.0 36.0 27.0 36.0 37 33.67405547517428 36.0 36.0 36.0 27.0 36.0 38 33.05010418219996 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 0.0 8 0.0 9 3.0 10 6.0 11 7.0 12 4.0 13 1.0 14 216.0 15 371.0 16 440.0 17 516.0 18 602.0 19 827.0 20 1007.0 21 1377.0 22 1935.0 23 2652.0 24 3841.0 25 5807.0 26 8047.0 27 11292.0 28 15724.0 29 20632.0 30 27738.0 31 36275.0 32 48692.0 33 72931.0 34 158768.0 35 324655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.82431579952627 17.639304924683135 11.486712784430614 25.04966649135998 2 16.60333330196909 20.426723946732764 37.34231067535725 25.62763207594089 3 18.544265361071847 25.107888924120363 28.750853168951636 27.596992545856153 4 12.166555608099394 15.497775246120115 36.2702054941748 36.06546365160569 5 14.451607876222347 37.03791275002787 32.92703733507799 15.583442038671786 6 33.16294247326923 36.03303209304013 17.195684386868308 13.608341046822334 7 29.767937364314424 31.098193366721837 21.092658470004082 18.041210798959654 8 28.576744710921005 31.9367943640477 19.907733313271464 19.578727611759838 9 28.05551562602856 13.833633140641647 19.064386250330816 39.04646498299898 10 16.569402686312916 26.212374020307216 30.60618029302789 26.612043000351974 11 37.47442409716895 21.402076702854693 22.217415661655153 18.9060835383212 12 24.970275880136228 24.392183732005993 28.36225137537029 22.27528901248749 13 29.362272729715638 19.232588261217735 25.67690670564904 25.728232303417585 14 24.178459578961796 20.33661022459939 25.095827908795208 30.38910228764361 15 25.217350407825954 27.002915922009162 23.058761875327537 24.72097179483734 16 25.466857453797942 25.824919217772763 24.13819974875216 24.570023579677137 17 23.575493311654718 25.88104084224105 25.52562801916139 25.017837826942845 18 25.064253019186562 24.183990015087538 27.052368393829024 23.69938857189688 19 25.281301180020666 25.044571542195516 26.13605370887629 23.538073568907535 20 25.740767859374014 23.71921556193565 25.658814852034517 24.881201726655817 21 27.61118231723538 23.959114249554624 24.55993271693013 23.86977071627987 22 26.08757570709215 24.051209432552838 25.29208024548713 24.56913461486788 23 23.84494878381719 23.910111031095155 26.138794243150578 26.106145941937083 24 24.387917158205042 25.477318471183537 25.766867044132308 24.367897326479117 25 24.542960257764236 24.411014637633254 25.833931253913356 25.212093850689154 26 24.09242254452229 25.711805695520496 26.536949400323017 23.6588223596342 27 25.25863227193336 24.710466209861615 25.492946392583637 24.537955125621387 28 23.913741517436698 24.778019161087755 26.543655224022967 24.764584097452577 29 23.73129114604326 25.2801289802499 26.4316807738815 24.556899099825337 30 23.823636493096654 25.37452189765822 26.776552396792365 24.025289212452762 31 24.65905258872828 25.366675489001416 24.993450424076645 24.980821498193663 32 23.99481402363247 25.29789134976455 25.067612502771002 25.63968212383198 33 23.683301872216365 24.834577223047003 25.802325659507865 25.67979524522877 34 24.79285321767778 25.18719458697612 25.95894819012763 24.06100400521847 35 25.76822304333221 24.808196170641587 25.916560295599595 23.5070204904266 36 23.98099578639608 26.027603405279905 25.226266230974286 24.76513457734973 37 25.286310822575963 25.818625923019873 25.20636950890506 23.688693745499105 38 24.01181217142696 25.466703031100995 25.84288683450734 24.678597962964705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1081.0 1 739.5 2 398.0 3 398.0 4 831.0 5 1264.0 6 1264.0 7 1419.0 8 1574.0 9 1473.5 10 1373.0 11 1373.0 12 1783.0 13 2193.0 14 2627.5 15 3062.0 16 3062.0 17 4329.0 18 5596.0 19 5596.0 20 6358.5 21 7121.0 22 7015.0 23 6909.0 24 6909.0 25 7522.0 26 8135.0 27 8135.0 28 10339.5 29 12544.0 30 15010.5 31 17477.0 32 17477.0 33 21858.0 34 26239.0 35 26239.0 36 28332.5 37 30426.0 38 35367.5 39 40309.0 40 40309.0 41 42327.5 42 44346.0 43 51207.0 44 58068.0 45 58068.0 46 59568.5 47 61069.0 48 61069.0 49 64296.5 50 67524.0 51 67303.5 52 67083.0 53 67083.0 54 62920.0 55 58757.0 56 58757.0 57 57761.5 58 56766.0 59 50378.5 60 43991.0 61 43991.0 62 43584.0 63 43177.0 64 35422.5 65 27668.0 66 27668.0 67 23175.5 68 18683.0 69 18683.0 70 15242.5 71 11802.0 72 9171.0 73 6540.0 74 6540.0 75 4875.5 76 3211.0 77 3211.0 78 3321.5 79 3432.0 80 2739.5 81 2047.0 82 2047.0 83 2121.0 84 2195.0 85 2195.0 86 1473.5 87 752.0 88 661.0 89 570.0 90 570.0 91 408.5 92 247.0 93 225.5 94 204.0 95 204.0 96 182.5 97 161.0 98 161.0 99 268.0 100 375.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12225119530770358 2 0.05669231254928671 3 0.011419067693576708 4 0.0022838135387153414 5 2.686839457312167E-4 6 2.686839457312167E-4 7 1.3434197286560834E-4 8 6.717098643280416E-4 9 2.686839457312167E-4 10 4.03025918596825E-4 11 0.0021494715658497334 12 0.0032242073487746 13 0.013568539259426441 14 0.00886657020913015 15 0.02404721314294389 16 0.011284725720711099 17 0.022032083549959766 18 0.006179730751817983 19 0.008060518371936498 20 0.005373678914624333 21 0.006851440616146025 22 0.007120124561877242 23 0.009806964019189407 24 0.014374591096620091 25 0.016524062662469825 26 0.018136166336857124 27 0.009269596127726975 28 0.0064484146975492 29 0.009269596127726975 30 0.0026868394573121663 31 0.006179730751817983 32 0.007254466534742849 33 0.008597886263398934 34 0.012090777557904749 35 0.01598669477100739 36 0.015180642933813741 37 0.009806964019189407 38 0.006985782589011632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 744369.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.429581271804025 #Duplication Level Percentage of deduplicated Percentage of total 1 80.6417523767837 40.66729805385709 2 12.016772744022509 12.120016354389653 3 3.320256677166522 5.023174618332574 4 1.3129153300275356 2.648390813544841 5 0.6715375009593316 1.6932677490846395 6 0.386771360618578 1.1702830658352479 7 0.26553055809055726 0.9373416401562668 8 0.17414901770739277 0.7025809633503841 9 0.13214363806359444 0.5997553501751903 >10 0.8078209194959822 7.947117785052864 >50 0.11659009941898571 4.165607880051747 >100 0.13637823756370537 14.531612626164526 >500 0.012300782211614047 4.256901194052208 >1k 0.005080757870014498 3.5366519059528376 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 3706 0.49787135143994443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 2635 0.3539910985008779 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1495 0.20084124943408446 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 1348 0.18109297942284003 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 1312 0.17625666839967813 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA 1286 0.17276377710517232 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 1254 0.16846483397347284 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 1192 0.16013563165580513 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.1578518181170898 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 1155 0.1551649786597776 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC 1153 0.1548962947140464 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC 1150 0.15449326879544956 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 1146 0.15395590090398714 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 1072 0.1440145949119321 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG 1055 0.1417307813732168 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA 1051 0.14119341348175435 No Hit CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT 1032 0.1386409159973078 No Hit GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG 1005 0.13501368272993636 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG 1004 0.13487934075707075 No Hit GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA 953 0.12802790014092474 No Hit ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA 947 0.12722184830373107 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC 943 0.12668448041226865 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 937 0.12587842857507497 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT 923 0.1239976409549565 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC 879 0.11808659414886973 No Hit GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT 853 0.11459370285436389 No Hit GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT 849 0.11405633496290146 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 845 0.11351896707143902 No Hit GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 840 0.11284725720711099 No Hit GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG 806 0.10827963012968031 No Hit ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT 804 0.10801094618394907 No Hit GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG 791 0.10626450053669617 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT 789 0.10599581659096496 No Hit CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC 761 0.10223424135072792 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC 750 0.10075647964920625 No Hit CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG 750 0.10075647964920625 No Hit CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT 747 0.10035345373060942 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.343419728656083E-4 2 0.0 0.0 0.0 0.0 1.343419728656083E-4 3 0.0 0.0 0.0 0.0 1.343419728656083E-4 4 0.0 0.0 0.0 0.0 1.343419728656083E-4 5 0.0 0.0 0.0 0.0 1.343419728656083E-4 6 0.0 0.0 0.0 0.0 1.343419728656083E-4 7 0.0 0.0 0.0 0.0 1.343419728656083E-4 8 0.0 0.0 0.0 0.0 1.343419728656083E-4 9 0.0 0.0 0.0 0.0 1.343419728656083E-4 10 0.0 0.0 0.0 0.0 1.343419728656083E-4 11 0.0 0.0 0.0 0.0 1.343419728656083E-4 12 0.0 0.0 0.0 0.0 1.343419728656083E-4 13 0.0 0.0 0.0 1.343419728656083E-4 1.343419728656083E-4 14 0.0 0.0 0.0 1.343419728656083E-4 1.343419728656083E-4 15 0.0 0.0 0.0 1.343419728656083E-4 1.343419728656083E-4 16 0.0 0.0 0.0 1.343419728656083E-4 1.343419728656083E-4 17 0.0 0.0 0.0 1.343419728656083E-4 1.343419728656083E-4 18 0.0 0.0 0.0 2.686839457312166E-4 1.343419728656083E-4 19 0.0 0.0 0.0 4.0302591859682496E-4 1.343419728656083E-4 20 0.0 0.0 0.0 6.717098643280416E-4 1.343419728656083E-4 21 0.0 0.0 0.0 9.403938100592583E-4 1.343419728656083E-4 22 0.0 0.0 0.0 0.0014777617015216916 1.343419728656083E-4 23 0.0 0.0 0.0 0.0038959172131026415 1.343419728656083E-4 24 0.0 0.0 0.0 0.0060453887789523745 1.343419728656083E-4 25 0.0 0.0 0.0 0.006717098643280416 1.343419728656083E-4 26 0.0 0.0 0.0 0.00953828007345819 1.343419728656083E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 40 0.004460334 20.016768 1 CTATACT 50 7.194407E-4 19.199314 4 GTATTGA 80 2.7712267E-6 18.01509 1 GTGTTAG 45 0.00880481 17.792683 1 TGTTAGG 45 0.008832132 17.783117 2 AGGCCCG 330 0.0 16.970232 10 GTCCTAG 85 4.9395967E-6 16.95538 1 CTAGCGG 230 0.0 16.695057 29 CGAGCCG 345 0.0 16.232395 15 TCTAGAC 70 3.6914367E-4 16.00158 3 TAGCGGC 230 0.0 15.99943 30 AACGAAT 60 0.0024414023 15.999429 31 GAGCCGC 385 0.0 15.376075 16 GATATAC 125 5.075526E-8 15.372878 1 TCTAGCG 240 0.0 15.331756 28 TCTAGAT 220 0.0 15.277314 2 ATTATTA 95 1.4315261E-5 15.157354 21 ACCGTCG 190 1.8189894E-12 15.156337 8 CTTAAGA 75 6.22649E-4 14.937817 2 CCGAGCC 365 0.0 14.904578 14 >>END_MODULE