FastQCFastQC Report
Thu 2 Feb 2017
SRR4062966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744369
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48100.646184889483576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36830.49478148606403544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21300.28614840220374577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.21145426529046749No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13980.18781007806612043No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12740.171151673430785No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12590.16913654383780088No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12450.16725575621768235No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12440.16712141424481675No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11740.15771747614422418No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11680.1569114243070305No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11640.1563740564155681No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11590.15570234655124005No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10740.14428327885766332No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10680.1434772270204697No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10470.14065604559029193No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10350.1390439419159046No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10180.13676012837718926No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10050.13501368272993636No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9900.13299855313695222No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9860.1324611852454898No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9310.12507237673788135No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9260.1244006668735533No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9220.12386329898209088No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9170.12319158911776283No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8960.12037040768758506No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC8560.11499672877296073No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8490.11405633496290146No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8480.11392199299003587No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8460.11365330904430464No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT8260.11096646958699247No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT8180.1098917338040676No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG8100.10881699802114274No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.10760792026535226No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC7900.10613015856383058No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7680.1031746351607872No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7660.10290595121505597No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7650.10277160924219036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTA451.2368257E-524.8901445
ACCGTCG2350.020.4265618
ATACCGT2300.020.174936
TACCGTC2300.020.174937
TCATATA400.004482457419.9996642
ATATAAG507.1902416E-419.2009681
CCGTCGT2350.019.064799
GTCGTAG2400.018.66760611
AGATACC2550.018.1957744
AGTGTAT1159.385985E-1018.08786810
CGTCGTA2500.017.92090210
TATATGG450.00884828117.7774812
TAACACC450.00884828117.7774814
GTCTAAA550.001363573417.4554251
TCTATAG550.001364187317.4542523
AGGGGCG2500.017.2797132
AAGACGG3900.017.2316385
CAGATAC2800.017.142573
ACGGACC3850.017.0398188
GACGGAC3850.017.0398187
Produced by FastQC (version 0.11.5)