##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744369
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126888680211025	32.0	32.0	32.0	32.0	32.0
2	31.318511383467072	32.0	32.0	32.0	32.0	32.0
3	31.39603207548944	32.0	32.0	32.0	32.0	32.0
4	31.49299742466438	32.0	32.0	32.0	32.0	32.0
5	31.412803327381983	32.0	32.0	32.0	32.0	32.0
6	34.93588932370908	36.0	36.0	36.0	36.0	36.0
7	34.956200486586624	36.0	36.0	36.0	36.0	36.0
8	34.90103161200963	36.0	36.0	36.0	36.0	36.0
9	34.99695984115405	36.0	36.0	36.0	36.0	36.0
10	34.85403475964206	36.0	36.0	36.0	32.0	36.0
11	35.03447483707677	36.0	36.0	36.0	36.0	36.0
12	34.91467135251468	36.0	36.0	36.0	32.0	36.0
13	34.971437553149045	36.0	36.0	36.0	36.0	36.0
14	34.90561536012381	36.0	36.0	36.0	32.0	36.0
15	34.888231508835	36.0	36.0	36.0	32.0	36.0
16	34.894630217002586	36.0	36.0	36.0	32.0	36.0
17	34.85187051045919	36.0	36.0	36.0	32.0	36.0
18	34.83760339294086	36.0	36.0	36.0	32.0	36.0
19	34.842485380234805	36.0	36.0	36.0	32.0	36.0
20	34.823583464652614	36.0	36.0	36.0	32.0	36.0
21	34.80964279812835	36.0	36.0	36.0	32.0	36.0
22	34.78911937493367	36.0	36.0	36.0	32.0	36.0
23	34.73349239422921	36.0	36.0	36.0	32.0	36.0
24	34.705379992987346	36.0	36.0	36.0	32.0	36.0
25	34.68231750650551	36.0	36.0	36.0	32.0	36.0
26	34.620978305114804	36.0	36.0	36.0	32.0	36.0
27	34.60830985707358	36.0	36.0	36.0	32.0	36.0
28	34.56803682044792	36.0	36.0	36.0	32.0	36.0
29	34.52839653451447	36.0	36.0	36.0	32.0	36.0
30	34.50060521058776	36.0	36.0	36.0	32.0	36.0
31	34.495219440895575	36.0	36.0	36.0	32.0	36.0
32	34.46350667478092	36.0	36.0	36.0	32.0	36.0
33	34.40429679366014	36.0	36.0	36.0	32.0	36.0
34	34.375962728163046	36.0	36.0	36.0	32.0	36.0
35	34.332102760861886	36.0	36.0	36.0	32.0	36.0
36	34.29879804236877	36.0	36.0	36.0	32.0	36.0
37	34.268898892887805	36.0	36.0	36.0	32.0	36.0
38	33.83127857285835	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	5.0
21	24.0
22	73.0
23	198.0
24	521.0
25	1324.0
26	2654.0
27	5406.0
28	9315.0
29	14418.0
30	21668.0
31	30850.0
32	43402.0
33	68186.0
34	164121.0
35	382203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.233885789830076	18.823154806049075	12.190721095454595	25.752238308666257
2	15.646427573458292	21.363895277604623	36.61428218300625	26.37539496593083
3	17.613307378464178	25.717083865663398	29.123861955562365	27.545746800310063
4	11.753104995533105	16.21153600720077	36.348968583960826	35.686390413305304
5	13.81252283816607	37.44639748081594	32.85323925800142	15.887840423016572
6	33.21385821778773	36.61876466850357	16.635476854831015	13.531900258877677
7	29.186084783674676	31.303594338798842	21.21714481470784	18.293176062818645
8	28.25211088794997	32.17295510878546	19.77255476217665	19.802379241087923
9	27.783256128886645	13.582160936341262	19.007475038423095	39.627107896349
10	16.248611221198374	26.30531772975137	30.741617955851947	26.70445309319831
11	37.47858809746765	21.263446674790316	22.237075271822004	19.02088995592003
12	24.60399238547652	24.522849615848095	28.450885114466384	22.422272884209
13	29.454611892757487	19.136073640895845	25.7002911190552	25.709023347291467
14	23.940276932542865	20.10199242579957	25.40635088242525	30.551379759232315
15	25.230228555998437	27.107120258903848	23.056038067141433	24.606613117956282
16	25.571860192995675	25.66267536665283	24.06333417968776	24.70213026066373
17	23.495201976439102	25.80494351591751	25.65286840263364	25.046986105009744
18	24.996339181239414	23.84193860840524	27.34356213114732	23.818160079208027
19	25.35006159579456	24.605538382173357	26.504730852574465	23.53966916945762
20	25.65045024712206	23.274478115020912	26.14845594053487	24.92661569732216
21	27.42111428755669	23.728854545063733	24.984147625905468	23.86588354147411
22	25.711844528721645	23.90212381224903	25.83879769307964	24.547233965949683
23	23.688788759338447	23.393102077061243	26.65841806953272	26.259691094067595
24	24.23072427787831	25.214644887146026	26.18634037688297	24.36829045809269
25	24.567116577933792	23.96029388649984	26.401959243332275	25.070630292234092
26	24.142058575787008	25.22095895987071	26.85254222032352	23.78444024401876
27	25.24782735444383	24.31979300588821	25.85559043968784	24.576789199980116
28	23.719821755070402	24.330271679771727	27.131033130073927	24.818873435083944
29	23.856850567393323	24.507737425927196	27.15830455056565	24.477107456113835
30	23.908437884973715	24.778839527169993	27.31696242051993	23.99576016733636
31	24.58861129359229	24.722147214620705	25.533438388756114	25.155803103030888
32	23.961099938337036	24.83526315577355	25.604236608456286	25.599400297433128
33	23.71619452180303	24.16744920865861	26.414990414700235	25.701365854838127
34	24.646512683897367	24.502498088985437	26.75783112945327	24.093158097663927
35	25.760745006844722	24.210169956029873	26.423856984909367	23.605228052216038
36	24.00126818822385	25.371018943561594	25.90099802651642	24.726714841698136
37	25.42260626114199	25.143443641527252	25.6323140807852	23.801636016545558
38	24.09584493712124	24.733163256395684	26.37844939808079	24.792542408402284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	452.0
1	345.0
2	238.0
3	238.0
4	800.0
5	1362.0
6	1362.0
7	1521.0
8	1680.0
9	1620.0
10	1560.0
11	1560.0
12	1945.5
13	2331.0
14	2828.5
15	3326.0
16	3326.0
17	4982.0
18	6638.0
19	6638.0
20	7258.5
21	7879.0
22	7542.5
23	7206.0
24	7206.0
25	7428.0
26	7650.0
27	7650.0
28	9942.0
29	12234.0
30	14643.0
31	17052.0
32	17052.0
33	21778.0
34	26504.0
35	26504.0
36	28439.0
37	30374.0
38	35152.0
39	39930.0
40	39930.0
41	41982.0
42	44034.0
43	51156.5
44	58279.0
45	58279.0
46	59614.0
47	60949.0
48	60949.0
49	64440.5
50	67932.0
51	67183.5
52	66435.0
53	66435.0
54	62655.5
55	58876.0
56	58876.0
57	58257.0
58	57638.0
59	50948.5
60	44259.0
61	44259.0
62	43800.5
63	43342.0
64	35468.5
65	27595.0
66	27595.0
67	23232.5
68	18870.0
69	18870.0
70	15427.5
71	11985.0
72	9289.5
73	6594.0
74	6594.0
75	4794.0
76	2994.0
77	2994.0
78	3208.0
79	3422.0
80	2653.5
81	1885.0
82	1885.0
83	1903.5
84	1922.0
85	1922.0
86	1209.5
87	497.0
88	419.0
89	341.0
90	341.0
91	202.0
92	63.0
93	40.5
94	18.0
95	18.0
96	12.5
97	7.0
98	7.0
99	11.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007657492453339674
2	1.3434197286560834E-4
3	0.0
4	5.373678914624334E-4
5	1.3434197286560834E-4
6	2.686839457312167E-4
7	5.373678914624334E-4
8	0.0018807876201185165
9	0.0033585493216402077
10	0.0013434197286560832
11	0.005373678914624333
12	2.686839457312167E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3434197286560834E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	744369.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.82281246267428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7107943118852	37.64171555165416
2	13.186018201958005	12.611849512032943
3	3.7248637400589675	5.344003802695666
4	1.4495887713570232	2.772936478424214
5	0.7119647278091809	1.7024077829028699
6	0.4250500739195228	1.2196253983379508
7	0.2814858120027162	0.942301023881664
8	0.18777606820089188	0.718398375563967
9	0.14319326725075868	0.6163114289085568
>10	0.8850519387588025	8.192768267239959
>50	0.12508294469038558	4.2092821806444
>100	0.14625945511221677	14.423197472977872
>500	0.017505957947789016	5.617910694782123
>1k	0.0053647290485159885	3.987292029953643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4810	0.646184889483576	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3683	0.49478148606403544	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2130	0.28614840220374577	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1574	0.21145426529046749	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1398	0.18781007806612043	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1274	0.171151673430785	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1259	0.16913654383780088	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1245	0.16725575621768235	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1244	0.16712141424481675	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1174	0.15771747614422418	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1168	0.1569114243070305	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1164	0.1563740564155681	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1159	0.15570234655124005	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1074	0.14428327885766332	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1068	0.1434772270204697	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1047	0.14065604559029193	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1035	0.1390439419159046	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1018	0.13676012837718926	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1005	0.13501368272993636	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	990	0.13299855313695222	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	986	0.1324611852454898	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	931	0.12507237673788135	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	926	0.1244006668735533	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	922	0.12386329898209088	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	917	0.12319158911776283	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	896	0.12037040768758506	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	856	0.11499672877296073	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	849	0.11405633496290146	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	848	0.11392199299003587	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	846	0.11365330904430464	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	826	0.11096646958699247	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	818	0.1098917338040676	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	810	0.10881699802114274	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.10760792026535226	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	790	0.10613015856383058	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	768	0.1031746351607872	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	766	0.10290595121505597	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.10277160924219036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	2.686839457312166E-4
16	0.0	0.0	0.0	0.0	2.686839457312166E-4
17	0.0	0.0	0.0	0.0	2.686839457312166E-4
18	0.0	0.0	0.0	0.0	2.686839457312166E-4
19	0.0	0.0	0.0	1.343419728656083E-4	2.686839457312166E-4
20	0.0	0.0	0.0	4.0302591859682496E-4	2.686839457312166E-4
21	0.0	0.0	0.0	6.717098643280416E-4	2.686839457312166E-4
22	0.0	0.0	0.0	0.0013434197286560832	2.686839457312166E-4
23	0.0	0.0	0.0	0.003492891294505816	2.686839457312166E-4
24	0.0	0.0	0.0	0.0057767048332211576	2.686839457312166E-4
25	0.0	0.0	0.0	0.006448414697549199	2.686839457312166E-4
26	0.0	0.0	0.0	0.009135254154861366	2.686839457312166E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACTA	45	1.2368257E-5	24.890144	5
ACCGTCG	235	0.0	20.426561	8
ATACCGT	230	0.0	20.17493	6
TACCGTC	230	0.0	20.17493	7
TCATATA	40	0.0044824574	19.999664	2
ATATAAG	50	7.1902416E-4	19.200968	1
CCGTCGT	235	0.0	19.06479	9
GTCGTAG	240	0.0	18.667606	11
AGATACC	255	0.0	18.195774	4
AGTGTAT	115	9.385985E-10	18.087868	10
CGTCGTA	250	0.0	17.920902	10
TATATGG	45	0.008848281	17.777481	2
TAACACC	45	0.008848281	17.777481	4
GTCTAAA	55	0.0013635734	17.455425	1
TCTATAG	55	0.0013641873	17.454252	3
AGGGGCG	250	0.0	17.27971	32
AAGACGG	390	0.0	17.231638	5
CAGATAC	280	0.0	17.14257	3
ACGGACC	385	0.0	17.039818	8
GACGGAC	385	0.0	17.039818	7
>>END_MODULE