FastQCFastQC Report
Thu 2 Feb 2017
SRR4062965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446295
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT75151.6838638120525662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT51741.1593228694025253No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA31580.7076037150315374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29420.6592052342060745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.5263334789769099No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22070.49451595917498514No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17970.40264847242294893No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA17090.38293057282738996No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA14850.332739555675058No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA12580.2818763374001501No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA10740.24064800188216312No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.19807526411902443No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8540.19135325289326566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8410.18844038136210353No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8100.18149430309548617No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA7770.17410009074715155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.15796726380533055No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6970.15617472747846153No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6630.1485564480892683No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA6160.13802529716891293No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA6050.13556055971946807No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG5870.13152735298401283No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5840.13085515186143692No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5810.13018295073886108No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5510.12346093951310232No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5470.12256467134966781No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC5440.12189247022709195No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG5260.1178592634916367No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC5220.1169629953282022No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5040.11292978859274694No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG4930.11046505114330207No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4880.10934471593900895No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA4790.10732811257128133No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA4750.10643184440784684No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA4740.1062077773669882No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG4740.1062077773669882No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT4600.10307083879496745No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC4570.10239863767239159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA308.4195985E-426.6607828
CGGTCCA1500.024.53341910
ATAACCC401.5913839E-424.0000843
CTAGACC350.002068569422.8495394
GTCCTAG653.7728023E-722.1738051
CTAGCGG1600.022.00007629
CCGGTCC1900.021.0527069
GCATTCG1550.020.64523322
ACCTCTA1800.020.44451525
CCTCTAG1800.020.44451526
CGAACGA555.822892E-520.36370716
GTTGTTA555.822892E-520.36370712
GGACCGT555.8379832E-520.3568636
GGTCCAA1750.020.11435511
TCTAGCG1750.020.11435528
TCCTAGA400.00446755320.009042
CTATGAC400.004479164720.00006916
ACCGGAT400.004479164720.00006912
TAATAGA400.0044878919.9933454
AATACCT400.0044878919.9933455
Produced by FastQC (version 0.11.5)