##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062965_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446295
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.811496879866457	32.0	32.0	32.0	32.0	32.0
2	30.586394649279065	32.0	32.0	32.0	32.0	32.0
3	30.73000817844699	32.0	32.0	32.0	32.0	32.0
4	30.68156488421336	32.0	32.0	32.0	32.0	32.0
5	30.772491289393788	32.0	32.0	32.0	32.0	32.0
6	34.33161922047076	36.0	36.0	36.0	32.0	36.0
7	34.0983811156298	36.0	36.0	36.0	32.0	36.0
8	34.023016166436996	36.0	36.0	36.0	32.0	36.0
9	34.036511724307914	36.0	36.0	36.0	32.0	36.0
10	33.9602549882925	36.0	36.0	36.0	32.0	36.0
11	34.26472176475201	36.0	36.0	36.0	32.0	36.0
12	34.09175097189079	36.0	36.0	36.0	32.0	36.0
13	34.16501417224033	36.0	36.0	36.0	32.0	36.0
14	34.07952811481195	36.0	36.0	36.0	32.0	36.0
15	34.104742378919774	36.0	36.0	36.0	32.0	36.0
16	34.07769300574732	36.0	36.0	36.0	32.0	36.0
17	33.99497193560313	36.0	36.0	36.0	32.0	36.0
18	34.06228839668829	36.0	36.0	36.0	32.0	36.0
19	34.01290402088305	36.0	36.0	36.0	32.0	36.0
20	33.85065483592691	36.0	36.0	36.0	32.0	36.0
21	33.77274000380914	36.0	36.0	36.0	32.0	36.0
22	33.80075958726851	36.0	36.0	36.0	32.0	36.0
23	33.81989939389865	36.0	36.0	36.0	32.0	36.0
24	33.830484320908816	36.0	36.0	36.0	32.0	36.0
25	33.79822987037722	36.0	36.0	36.0	32.0	36.0
26	33.777008480937496	36.0	36.0	36.0	27.0	36.0
27	33.79188877312092	36.0	36.0	36.0	32.0	36.0
28	33.777124995798744	36.0	36.0	36.0	27.0	36.0
29	33.73809027661076	36.0	36.0	36.0	27.0	36.0
30	33.65387243863364	36.0	36.0	36.0	27.0	36.0
31	33.69692692053462	36.0	36.0	36.0	27.0	36.0
32	33.60679371267884	36.0	36.0	36.0	27.0	36.0
33	33.472068923021766	36.0	36.0	36.0	21.0	36.0
34	33.38873166851522	36.0	36.0	36.0	21.0	36.0
35	33.31580456872696	36.0	36.0	36.0	21.0	36.0
36	33.252357745437436	36.0	36.0	36.0	21.0	36.0
37	33.20766981480859	36.0	36.0	36.0	21.0	36.0
38	32.44247862960598	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	2.0
9	1.0
10	1.0
11	8.0
12	3.0
13	0.0
14	191.0
15	316.0
16	398.0
17	480.0
18	681.0
19	856.0
20	1110.0
21	1407.0
22	1829.0
23	2344.0
24	3071.0
25	4107.0
26	5458.0
27	7435.0
28	10059.0
29	13480.0
30	17582.0
31	23117.0
32	30464.0
33	43786.0
34	91145.0
35	186961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.85726848133808	16.78159785003163	12.257556519671404	22.103577148958887
2	16.0026724180451	20.573583416659567	38.28575143821827	25.137992727077062
3	19.057575431614048	27.95474103157102	27.38440990336865	25.60327363344628
4	11.855481835516924	15.272372015066674	34.161507384327884	38.71063876508852
5	13.57378768255903	38.61311153634151	30.631153454897444	17.18194732620201
6	31.993555803026265	35.042897827212165	18.078706141481046	14.884840228280524
7	29.733472254898665	31.801162907942054	20.037195128782532	18.428169708376746
8	31.38811086961393	29.120302942033206	18.795850231911984	20.69573595644088
9	29.87012987012987	13.08201805096215	18.96471368521058	38.0831383936974
10	18.008021689932555	26.203141455107666	28.47027717403482	27.31855968092496
11	36.82139624095867	22.516379704403473	20.774887739645607	19.887336314992247
12	26.68844656865711	25.18402000524331	26.983774869309386	21.1437585567902
13	31.23994316628344	19.757784381485116	25.587274267503936	23.414998184727505
14	25.96583931660706	19.581887935748156	26.088417995536073	28.363854752108715
15	25.635571852173932	25.804788725449544	26.003366407200794	22.55627301517573
16	24.48720735579843	26.106540753373235	25.411388220951835	23.994863669876498
17	22.299019047555017	25.535019128236502	26.386881189783036	25.779080634425444
18	24.190512470029354	23.58460125036413	29.465794250117643	22.759092029488876
19	24.18197544917962	25.03798251235832	29.05337271264605	21.726669325816008
20	26.366679812846204	21.52537511428213	28.869234354552464	23.2387107183192
21	28.144362007497755	22.396009563781323	26.390255161147923	23.069373267573003
22	25.7913005676076	23.127376702766778	27.401128044476415	23.680194685149207
23	23.04867035520687	23.962738124850137	28.51985559566787	24.46873592427512
24	23.36324397491809	25.749745640023487	28.029572988987443	22.857437396070978
25	24.43327872489674	24.336238561923054	27.524444932508903	23.706037780671302
26	23.1003346055666	25.528858329392623	28.731031360587362	22.639775704453413
27	24.00375573388071	25.43837436778853	27.89282264913692	22.66504724919384
28	22.430842660750898	25.23545427044469	28.943116757980125	23.390586310824286
29	21.85566548273969	26.172031685919485	29.326730232714482	22.645572598626345
30	22.511825509040268	26.223836551410436	28.84953650278636	22.414801436762936
31	22.528514128218344	26.92025007282587	26.796558137450422	23.754677661505365
32	22.07318138936631	27.096923101055214	26.66600935327084	24.163886156307633
33	21.944152506100828	26.366775648682015	27.289126522965773	24.39994532225138
34	23.067786582523574	26.706317903115924	27.40350138047259	22.82239413388791
35	23.875669824450526	26.661646485088493	27.299702599064542	22.16298109139644
36	22.49171936838614	28.552603860858493	26.260471245254603	22.695205525500757
37	23.7144336157654	28.567394894709835	26.320412869221478	21.397758620303286
38	22.164085625869713	28.77940784328664	26.25737506918504	22.799131461658604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	238.0
2	309.0
3	309.0
4	803.5
5	1298.0
6	1298.0
7	1798.0
8	2298.0
9	2412.0
10	2526.0
11	2526.0
12	3095.5
13	3665.0
14	4242.5
15	4820.0
16	4820.0
17	6806.5
18	8793.0
19	8793.0
20	9615.0
21	10437.0
22	8283.0
23	6129.0
24	6129.0
25	5260.0
26	4391.0
27	4391.0
28	6081.5
29	7772.0
30	9906.5
31	12041.0
32	12041.0
33	12504.5
34	12968.0
35	12968.0
36	13852.5
37	14737.0
38	17295.0
39	19853.0
40	19853.0
41	21917.0
42	23981.0
43	28595.5
44	33210.0
45	33210.0
46	36776.0
47	40342.0
48	40342.0
49	40096.5
50	39851.0
51	37480.0
52	35109.0
53	35109.0
54	32949.0
55	30789.0
56	30789.0
57	30705.0
58	30621.0
59	27904.0
60	25187.0
61	25187.0
62	24751.0
63	24315.0
64	20403.5
65	16492.0
66	16492.0
67	14599.5
68	12707.0
69	12707.0
70	10415.5
71	8124.0
72	6528.0
73	4932.0
74	4932.0
75	3810.5
76	2689.0
77	2689.0
78	2458.5
79	2228.0
80	1670.5
81	1113.0
82	1113.0
83	1170.5
84	1228.0
85	1228.0
86	796.5
87	365.0
88	316.5
89	268.0
90	268.0
91	202.5
92	137.0
93	109.5
94	82.0
95	82.0
96	90.5
97	99.0
98	99.0
99	160.5
100	222.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11673892828734356
2	0.0575852295006666
3	0.014116223574093367
4	0.0026888044903034987
5	2.240670408586249E-4
6	4.481340817172498E-4
7	0.0
8	0.0011203352042931246
9	6.722011225758747E-4
10	0.0011203352042931246
11	0.002464737449444874
12	0.0026888044903034987
13	0.018149430309548617
14	0.01142741908378987
15	0.027336178984752237
16	0.013892156533234744
17	0.02151043592242799
18	0.0056016760214656226
19	0.008290480511769121
20	0.006946078266617372
21	0.006273877144041497
22	0.008066413470910496
23	0.010755217961213995
24	0.017253162146114116
25	0.01971789959555899
26	0.02195857000414524
27	0.009858949797779496
28	0.006722011225758748
29	0.008962681634344997
30	0.0031369385720207486
31	0.0056016760214656226
32	0.007618279389193247
33	0.009858949797779496
34	0.01590875990096237
35	0.02151043592242799
36	0.016356893982679616
37	0.011651486124648495
38	0.006273877144041497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	446295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.66505707846745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62711486896089	29.12720714512239
2	14.499266950906103	11.792270363090667
3	5.785211578097044	7.057678771029812
4	2.697261391666309	4.387371537906279
5	1.5210583889250568	3.092696310265959
6	0.9100873730977465	2.2205252984047417
7	0.5806732309379983	1.6529177056022268
8	0.41215196761094963	1.3408146630321547
9	0.2715260484912801	0.9937460034169763
>10	1.339663516155485	10.1360158232052
>50	0.17634426532953434	5.158911176360717
>100	0.16315964736097038	13.419022064765715
>500	0.010437822558445916	2.7696782905867576
>1k	0.004944231738211223	4.016457976000912
>5k	0.0010987181640469386	2.834686871209499
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7515	1.6838638120525662	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5174	1.1593228694025253	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3158	0.7076037150315374	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2942	0.6592052342060745	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2349	0.5263334789769099	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2207	0.49451595917498514	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1797	0.40264847242294893	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1709	0.38293057282738996	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1485	0.332739555675058	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1258	0.2818763374001501	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1074	0.24064800188216312	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.19807526411902443	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	854	0.19135325289326566	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	841	0.18844038136210353	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	810	0.18149430309548617	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	777	0.17410009074715155	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	705	0.15796726380533055	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	697	0.15617472747846153	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	663	0.1485564480892683	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	616	0.13802529716891293	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	605	0.13556055971946807	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	587	0.13152735298401283	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	584	0.13085515186143692	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	581	0.13018295073886108	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	551	0.12346093951310232	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	547	0.12256467134966781	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	544	0.12189247022709195	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	526	0.1178592634916367	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	522	0.1169629953282022	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	504	0.11292978859274694	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	493	0.11046505114330207	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	488	0.10934471593900895	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	479	0.10732811257128133	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	475	0.10643184440784684	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	474	0.1062077773669882	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	474	0.1062077773669882	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	460	0.10307083879496745	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	457	0.10239863767239159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.240670408586249E-4	0.0
6	0.0	0.0	0.0	2.240670408586249E-4	2.240670408586249E-4
7	0.0	0.0	0.0	2.240670408586249E-4	6.722011225758747E-4
8	0.0	0.0	0.0	2.240670408586249E-4	6.722011225758747E-4
9	0.0	0.0	0.0	2.240670408586249E-4	6.722011225758747E-4
10	0.0	0.0	0.0	2.240670408586249E-4	6.722011225758747E-4
11	0.0	0.0	0.0	2.240670408586249E-4	6.722011225758747E-4
12	0.0	0.0	0.0	4.481340817172498E-4	0.0015684692860103743
13	0.0	0.0	0.0	4.481340817172498E-4	0.002016603367727624
14	0.0	0.0	0.0	4.481340817172498E-4	0.002016603367727624
15	0.0	0.0	0.0	4.481340817172498E-4	0.002240670408586249
16	0.0	0.0	0.0	4.481340817172498E-4	0.002464737449444874
17	0.0	0.0	0.0	6.722011225758747E-4	0.0026888044903034987
18	0.0	0.0	0.0	6.722011225758747E-4	0.0026888044903034987
19	0.0	0.0	0.0	6.722011225758747E-4	0.002912871531162124
20	0.0	0.0	0.0	6.722011225758747E-4	0.0033610056128793734
21	0.0	0.0	0.0	0.0013444022451517493	0.0033610056128793734
22	0.0	0.0	0.0	0.0026888044903034987	0.0033610056128793734
23	0.0	0.0	0.0	0.0056016760214656226	0.0033610056128793734
24	0.0	0.0	0.0	0.009410815716062245	0.0033610056128793734
25	0.0	0.0	0.0	0.011651486124648495	0.0038091396945966233
26	0.0	0.0	0.0	0.016132826941820992	0.004705407858031123
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	30	8.4195985E-4	26.660782	8
CGGTCCA	150	0.0	24.533419	10
ATAACCC	40	1.5913839E-4	24.000084	3
CTAGACC	35	0.0020685694	22.849539	4
GTCCTAG	65	3.7728023E-7	22.173805	1
CTAGCGG	160	0.0	22.000076	29
CCGGTCC	190	0.0	21.052706	9
GCATTCG	155	0.0	20.645233	22
ACCTCTA	180	0.0	20.444515	25
CCTCTAG	180	0.0	20.444515	26
CGAACGA	55	5.822892E-5	20.363707	16
GTTGTTA	55	5.822892E-5	20.363707	12
GGACCGT	55	5.8379832E-5	20.356863	6
GGTCCAA	175	0.0	20.114355	11
TCTAGCG	175	0.0	20.114355	28
TCCTAGA	40	0.004467553	20.00904	2
CTATGAC	40	0.0044791647	20.000069	16
ACCGGAT	40	0.0044791647	20.000069	12
TAATAGA	40	0.00448789	19.993345	4
AATACCT	40	0.00448789	19.993345	5
>>END_MODULE