FastQCFastQC Report
Thu 2 Feb 2017
SRR4062965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446295
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT92562.0739645301874323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT68931.5444941126385014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT44961.0074054157003776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28140.6305246529761704No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA25340.5677858815357555No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22770.5102006520350889No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21440.4803997356008918No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA13880.31100505271177137No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA12920.28949461678934335No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA12270.27493025913353275No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.2401998678004459No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA10020.22451517494034215No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9950.2229467056543318No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA8820.1976271300373072No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8750.19605866075129677No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.19538645962872092No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8240.1846312416675069No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA7660.17163535329770666No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7250.16244860462250307No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7020.15729506268275467No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.15415812411073393No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6580.14743611288497518No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC6440.14429917431295444No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA6210.13914563237320607No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6120.13712902900547844No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC6070.13600869380118533No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5980.1339920904334577No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5750.12883854849370932No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5550.12435720767653682No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG5410.12122026910451607No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA5320.11920366573678845No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5260.1178592634916367No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5130.11494639196047457No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA5100.11427419083789869No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC5090.11405012379704008No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG5020.1124816545110297No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4710.10553557624441233No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4510.10105423542723982No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT4500.1008301683863812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACA350.002064908922.85652
TACCGTC1300.022.1532257
ATACCGT1350.021.3327376
AGATACC1500.020.26614
TCAGATA1500.020.26612
TCCGAAA801.2600503E-720.0016821
CGAAAAC801.2615237E-719.9994423
ACCGTCG1600.019.999448
TCTTACA507.1878586E-419.1994612
CACACCC507.1878586E-419.1994615
AGTAAAC1105.16593E-1018.90856226
TTCCGAA852.385732E-718.82511120
CCGTCGT1700.018.8230029
CGTCGTA1800.018.66614310
CGCGGTC953.7751306E-818.52579710
CCCTCGA1301.2732926E-1118.46102126
CAGATAC1650.018.4237273
CTCTCGG1352.3646862E-1117.77927215
ACCGGAA450.0088434617.77727926
GTCGTAG1800.017.77727911
Produced by FastQC (version 0.11.5)