##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446295
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.683267793723882	32.0	32.0	32.0	32.0	32.0
2	31.244497473644113	32.0	32.0	32.0	32.0	32.0
3	31.346849057237925	32.0	32.0	32.0	32.0	32.0
4	31.476081963723548	32.0	32.0	32.0	32.0	32.0
5	31.329577969728543	32.0	32.0	32.0	32.0	32.0
6	34.83570956430164	36.0	36.0	36.0	32.0	36.0
7	34.88725170571035	36.0	36.0	36.0	32.0	36.0
8	34.86102689924825	36.0	36.0	36.0	32.0	36.0
9	34.929338217994825	36.0	36.0	36.0	32.0	36.0
10	34.77676648853337	36.0	36.0	36.0	32.0	36.0
11	34.94934292340268	36.0	36.0	36.0	36.0	36.0
12	34.86482483559081	36.0	36.0	36.0	32.0	36.0
13	34.89411039783103	36.0	36.0	36.0	32.0	36.0
14	34.821355829664235	36.0	36.0	36.0	32.0	36.0
15	34.81115853863476	36.0	36.0	36.0	32.0	36.0
16	34.80201212202691	36.0	36.0	36.0	32.0	36.0
17	34.742481990611594	36.0	36.0	36.0	32.0	36.0
18	34.722537783304766	36.0	36.0	36.0	32.0	36.0
19	34.699246014407514	36.0	36.0	36.0	32.0	36.0
20	34.63422399982075	36.0	36.0	36.0	32.0	36.0
21	34.61507523050897	36.0	36.0	36.0	32.0	36.0
22	34.624808702763865	36.0	36.0	36.0	32.0	36.0
23	34.570214768258666	36.0	36.0	36.0	32.0	36.0
24	34.557781288161415	36.0	36.0	36.0	32.0	36.0
25	34.52197537503221	36.0	36.0	36.0	32.0	36.0
26	34.46737023717496	36.0	36.0	36.0	32.0	36.0
27	34.45311509203554	36.0	36.0	36.0	32.0	36.0
28	34.41089638019695	36.0	36.0	36.0	32.0	36.0
29	34.34578922013466	36.0	36.0	36.0	32.0	36.0
30	34.30812579123674	36.0	36.0	36.0	32.0	36.0
31	34.28368007707906	36.0	36.0	36.0	32.0	36.0
32	34.19939725966009	36.0	36.0	36.0	32.0	36.0
33	34.084067713059746	36.0	36.0	36.0	32.0	36.0
34	33.979905667775796	36.0	36.0	36.0	32.0	36.0
35	33.88509618077729	36.0	36.0	36.0	32.0	36.0
36	33.843771496431735	36.0	36.0	36.0	32.0	36.0
37	33.78574709553098	36.0	36.0	36.0	32.0	36.0
38	33.35129678799897	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	0.0
21	13.0
22	39.0
23	106.0
24	326.0
25	857.0
26	1939.0
27	3719.0
28	6562.0
29	10373.0
30	15478.0
31	22049.0
32	30463.0
33	46897.0
34	102901.0
35	204570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.09685920189308	18.132865440464666	13.13909504858198	22.63118030906027
2	15.440459785567842	21.539564637739613	37.127908670274145	25.8920669064184
3	18.421447697150988	27.928836307823303	28.085907303465195	25.563808691560514
4	11.51124594997916	16.048389626569033	34.15035828400113	38.290006139450675
5	13.094701934818897	38.501215563696654	30.939625135840643	17.464457365643803
6	32.13666327577785	35.53487163170466	17.198752391921023	15.129712700596468
7	28.757436224918496	32.06108067533806	20.29756103026025	18.883922069483187
8	30.8283346142901	29.398293900031597	18.960475574806008	20.8128959108723
9	29.262581424374766	12.70074595595073	19.024229563518293	39.01244305615621
10	17.783767917201633	26.27893584650305	28.62091604319174	27.316380193103573
11	36.858062231942384	22.175787534788668	20.77281262744641	20.193337605822535
12	26.143918260343497	25.575236110644305	27.2378135538153	21.043032075196898
13	31.378348401841833	19.60048846614907	25.792133006195456	23.229030125813644
14	25.60010755217961	19.349309313346552	26.526848833170884	28.52373430130295
15	25.731858972204485	25.75023246955489	26.025386795729283	22.492521762511345
16	24.36577681976455	25.65349298892658	25.551094121812078	24.429636069496787
17	22.154404597855677	25.284621158650666	27.015090915201828	25.545883328291826
18	24.04777109311106	22.39057125892067	30.540113601989717	23.021544045978555
19	24.12597049037072	24.22321558610336	30.05635286077594	21.594461062749975
20	25.87571001243572	20.878790934247526	30.176452794676166	23.069046258640586
21	27.564116927406594	21.872801337235096	27.737321138083864	22.825760597274442
22	25.310837002431125	22.408944756271076	28.777154124514055	23.503064116783744
23	22.849236491558276	22.785825518995285	29.925273641873652	24.439664347572794
24	23.136266370898174	24.560212415554734	29.407678777490226	22.895842436056867
25	24.13358876975991	23.270482528372487	28.856697924018864	23.739230777848732
26	22.966475014228287	24.244556279044755	30.13596418504394	22.653004521683016
27	24.133140635678192	24.08519028893445	29.228201077762467	22.55346799762489
28	22.374662499019706	23.64893176038271	30.47916736687617	23.49723837372142
29	21.933026361487357	24.679640148332382	30.69964933508106	22.687684155099205
30	22.496779036287656	24.661266650981975	30.445109176665657	22.396845136064712
31	22.64802428886723	25.18647979475459	28.32140176340761	23.84409415297057
32	22.16762455326634	25.171467303017064	28.49707032344077	24.163837820275827
33	22.03049552426086	24.672694070065766	28.929071578216202	24.367738827457174
34	23.013925766589363	25.01775731298805	29.042225433849804	22.926091486572783
35	24.180642848340224	24.892503837148077	28.868573477184373	22.058279837327326
36	22.690372959589507	26.610201772370296	27.938471190580223	22.760954077459978
37	23.912882734514167	26.72380376208562	27.826661737191767	21.53665176620845
38	22.49991597485968	26.532898643274066	27.985525269160533	22.981660112705722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	98.5
2	132.0
3	132.0
4	629.5
5	1127.0
6	1127.0
7	1708.0
8	2289.0
9	2575.5
10	2862.0
11	2862.0
12	3537.0
13	4212.0
14	4762.0
15	5312.0
16	5312.0
17	7942.5
18	10573.0
19	10573.0
20	11033.5
21	11494.0
22	9265.0
23	7036.0
24	7036.0
25	5440.5
26	3845.0
27	3845.0
28	5258.0
29	6671.0
30	8524.5
31	10378.0
32	10378.0
33	11522.0
34	12666.0
35	12666.0
36	13551.5
37	14437.0
38	16696.0
39	18955.0
40	18955.0
41	21054.5
42	23154.0
43	27509.5
44	31865.0
45	31865.0
46	36247.0
47	40629.0
48	40629.0
49	40953.0
50	41277.0
51	38462.5
52	35648.0
53	35648.0
54	33188.0
55	30728.0
56	30728.0
57	30717.0
58	30706.0
59	28031.0
60	25356.0
61	25356.0
62	24962.0
63	24568.0
64	20675.5
65	16783.0
66	16783.0
67	14969.0
68	13155.0
69	13155.0
70	10675.0
71	8195.0
72	6597.0
73	4999.0
74	4999.0
75	3763.5
76	2528.0
77	2528.0
78	2309.5
79	2091.0
80	1588.0
81	1085.0
82	1085.0
83	989.5
84	894.0
85	894.0
86	594.5
87	295.0
88	241.5
89	188.0
90	188.0
91	119.5
92	51.0
93	36.5
94	22.0
95	22.0
96	20.5
97	19.0
98	19.0
99	12.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008738614593486372
2	0.0
3	0.0
4	2.240670408586249E-4
5	0.0
6	2.240670408586249E-4
7	0.0
8	0.0031369385720207486
9	0.004929474898889748
10	0.0013444022451517493
11	0.006497944184900122
12	0.0
13	0.0
14	0.0
15	0.0
16	2.240670408586249E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	2.240670408586249E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	2.240670408586249E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	446295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.32314703380856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.30638520270702	25.49409258268599
2	16.142341785441033	12.049659918560149
3	6.443963618409581	7.215270048312415
4	3.1216964681165438	4.660461450977385
5	1.6955868501150901	3.164231865771891
6	1.0384740369984777	2.3255471504212157
7	0.6458588509333176	1.6873839399529602
8	0.42632931380922423	1.2729561331299508
9	0.2994772813422884	1.0059691144341134
>10	1.478856123814265	10.315386370728516
>50	0.1954046509771911	5.187537679432402
>100	0.18398099445852456	13.901577700530252
>500	0.014429881918315651	3.5527575611356914
>1k	0.0060124507992981874	4.543274986277995
>5k	0.0012024901598596375	3.6238934976490826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9256	2.0739645301874323	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6893	1.5444941126385014	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4496	1.0074054157003776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2814	0.6305246529761704	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2534	0.5677858815357555	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2277	0.5102006520350889	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2144	0.4803997356008918	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1388	0.31100505271177137	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1292	0.28949461678934335	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1227	0.27493025913353275	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1072	0.2401998678004459	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1002	0.22451517494034215	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	995	0.2229467056543318	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	882	0.1976271300373072	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	875	0.19605866075129677	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	0.19538645962872092	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	824	0.1846312416675069	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	766	0.17163535329770666	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	725	0.16244860462250307	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	702	0.15729506268275467	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.15415812411073393	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	658	0.14743611288497518	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	644	0.14429917431295444	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	621	0.13914563237320607	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	612	0.13712902900547844	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	607	0.13600869380118533	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	598	0.1339920904334577	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	575	0.12883854849370932	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	555	0.12435720767653682	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	541	0.12122026910451607	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	532	0.11920366573678845	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	526	0.1178592634916367	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	513	0.11494639196047457	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	510	0.11427419083789869	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	509	0.11405012379704008	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	502	0.1124816545110297	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	471	0.10553557624441233	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	451	0.10105423542723982	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	450	0.1008301683863812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.481340817172498E-4
2	0.0	0.0	0.0	0.0	8.962681634344996E-4
3	0.0	0.0	0.0	0.0	8.962681634344996E-4
4	0.0	0.0	0.0	0.0	8.962681634344996E-4
5	0.0	0.0	0.0	2.240670408586249E-4	8.962681634344996E-4
6	0.0	0.0	0.0	2.240670408586249E-4	0.0011203352042931246
7	0.0	0.0	0.0	2.240670408586249E-4	0.0013444022451517493
8	0.0	0.0	0.0	2.240670408586249E-4	0.0013444022451517493
9	0.0	0.0	0.0	2.240670408586249E-4	0.0013444022451517493
10	0.0	0.0	0.0	2.240670408586249E-4	0.0013444022451517493
11	0.0	0.0	0.0	2.240670408586249E-4	0.0013444022451517493
12	0.0	0.0	0.0	4.481340817172498E-4	0.002912871531162124
13	0.0	0.0	0.0	4.481340817172498E-4	0.0031369385720207486
14	0.0	0.0	0.0	4.481340817172498E-4	0.0031369385720207486
15	0.0	0.0	0.0	4.481340817172498E-4	0.0033610056128793734
16	0.0	0.0	0.0	4.481340817172498E-4	0.0035850726537379985
17	0.0	0.0	0.0	8.962681634344996E-4	0.004481340817172498
18	0.0	0.0	0.0	8.962681634344996E-4	0.004481340817172498
19	0.0	0.0	0.0	8.962681634344996E-4	0.004481340817172498
20	0.0	0.0	0.0	8.962681634344996E-4	0.005153541939748373
21	0.0	0.0	2.240670408586249E-4	0.0015684692860103743	0.005153541939748373
22	0.0	0.0	2.240670408586249E-4	0.002912871531162124	0.005377608980606997
23	0.0	0.0	2.240670408586249E-4	0.005825743062324248	0.0056016760214656226
24	4.481340817172498E-4	0.0	2.240670408586249E-4	0.00963488275692087	0.005825743062324248
25	4.481340817172498E-4	0.0	2.240670408586249E-4	0.01142741908378987	0.006049810103182872
26	4.481340817172498E-4	0.0	2.240670408586249E-4	0.016132826941820992	0.007170145307475997
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACA	35	0.0020649089	22.8565	2
TACCGTC	130	0.0	22.153225	7
ATACCGT	135	0.0	21.332737	6
AGATACC	150	0.0	20.2661	4
TCAGATA	150	0.0	20.2661	2
TCCGAAA	80	1.2600503E-7	20.00168	21
CGAAAAC	80	1.2615237E-7	19.99944	23
ACCGTCG	160	0.0	19.99944	8
TCTTACA	50	7.1878586E-4	19.199461	2
CACACCC	50	7.1878586E-4	19.199461	5
AGTAAAC	110	5.16593E-10	18.908562	26
TTCCGAA	85	2.385732E-7	18.825111	20
CCGTCGT	170	0.0	18.823002	9
CGTCGTA	180	0.0	18.666143	10
CGCGGTC	95	3.7751306E-8	18.525797	10
CCCTCGA	130	1.2732926E-11	18.461021	26
CAGATAC	165	0.0	18.423727	3
CTCTCGG	135	2.3646862E-11	17.779272	15
ACCGGAA	45	0.00884346	17.777279	26
GTCGTAG	180	0.0	17.777279	11
>>END_MODULE