FastQCFastQC Report
Thu 2 Feb 2017
SRR4062964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333378
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28430.21321785720178374No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24310.18231889231710738No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22720.17039429179122498No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20440.15329486462203518No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20210.15156992240759934No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19840.1487950153669852No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19690.14767005305322273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19560.14669508571462855No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19460.14594511083878692No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18710.14032029926997447No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18330.13747039474177616No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17870.13402051031290452No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16830.12622077160415127No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16330.12247089722494295No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15890.11917100777123965No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15600.11699608063129885No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15570.11677108816854635No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15330.11497114846652637No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14650.1098713193108031No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14610.10957132936046643No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14330.10747139970810977No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14210.10657142985709979No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT13930.10447150020474313No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13890.10417151025440648No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13560.101696593164129No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13460.10094661828828734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4900.020.56959528
CTAGCGG4850.020.4533229
TAGCGGC5100.018.82326530
ATCGTTT3550.018.47861329
AAGACGG5150.018.327095
ACTATAT701.7884024E-518.2847718
GTTCGGA550.001354669617.4739631
CGCTTCG3500.017.37118332
AGCGGCG5750.017.25193231
CTATACA1302.6011548E-1017.2279424
CGTTTAT3750.017.06642731
CAAGACG5450.017.0247294
GTAATAC854.9875853E-616.9403023
AAACGCT3800.016.8418729
ACCGTCG4300.016.7433228
CGCAAGA5900.016.2764512
CATCGTT3950.016.2010928
GTATTAG4400.016.01781
CTATTAG703.6657855E-416.01781
TCTATAC805.6244182E-515.9991763
Produced by FastQC (version 0.11.5)