##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062964_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1333378
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272510870885824	32.0	32.0	32.0	32.0	32.0
2	30.904414952099106	32.0	32.0	32.0	32.0	32.0
3	30.95895312507031	32.0	32.0	32.0	32.0	32.0
4	31.022879483537302	32.0	32.0	32.0	32.0	32.0
5	30.941149471492704	32.0	32.0	32.0	32.0	32.0
6	34.58450716900984	36.0	36.0	36.0	32.0	36.0
7	34.516709440233754	36.0	36.0	36.0	32.0	36.0
8	34.509390435420414	36.0	36.0	36.0	32.0	36.0
9	34.6394720776854	36.0	36.0	36.0	32.0	36.0
10	34.37133506027548	36.0	36.0	36.0	32.0	36.0
11	34.620903449734435	36.0	36.0	36.0	32.0	36.0
12	34.44478834959029	36.0	36.0	36.0	32.0	36.0
13	34.53535231569742	36.0	36.0	36.0	32.0	36.0
14	34.433475728563096	36.0	36.0	36.0	32.0	36.0
15	34.38641780500353	36.0	36.0	36.0	32.0	36.0
16	34.401249308148174	36.0	36.0	36.0	32.0	36.0
17	34.31024960663818	36.0	36.0	36.0	32.0	36.0
18	34.343089506501535	36.0	36.0	36.0	32.0	36.0
19	34.33811042330082	36.0	36.0	36.0	32.0	36.0
20	34.32204521148542	36.0	36.0	36.0	32.0	36.0
21	34.29339092140413	36.0	36.0	36.0	32.0	36.0
22	34.28025136157939	36.0	36.0	36.0	32.0	36.0
23	34.22532170172299	36.0	36.0	36.0	32.0	36.0
24	34.20001229958796	36.0	36.0	36.0	32.0	36.0
25	34.169108084879156	36.0	36.0	36.0	32.0	36.0
26	34.123983596549515	36.0	36.0	36.0	32.0	36.0
27	34.124594076098454	36.0	36.0	36.0	32.0	36.0
28	34.118144292166214	36.0	36.0	36.0	32.0	36.0
29	34.08076629432914	36.0	36.0	36.0	32.0	36.0
30	34.051929760353026	36.0	36.0	36.0	32.0	36.0
31	34.07581721012346	36.0	36.0	36.0	32.0	36.0
32	34.03128370199598	36.0	36.0	36.0	32.0	36.0
33	33.98183860840662	36.0	36.0	36.0	32.0	36.0
34	33.993801457651166	36.0	36.0	36.0	32.0	36.0
35	33.945756567155	36.0	36.0	36.0	32.0	36.0
36	33.90972252429544	36.0	36.0	36.0	32.0	36.0
37	33.906748123937845	36.0	36.0	36.0	32.0	36.0
38	33.292988934870685	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	1.0
8	4.0
9	6.0
10	13.0
11	10.0
12	12.0
13	12.0
14	113.0
15	296.0
16	381.0
17	464.0
18	646.0
19	795.0
20	1135.0
21	1651.0
22	2347.0
23	3589.0
24	5358.0
25	8127.0
26	11981.0
27	17525.0
28	24687.0
29	33553.0
30	45793.0
31	62574.0
32	85507.0
33	129440.0
34	291617.0
35	605738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78014083216824	17.172273873365178	11.132318865173247	25.915266429293332
2	17.01042183324917	19.428026019691107	36.896546290453166	26.665005856606562
3	18.948896962860065	23.50258170395461	28.341814003306258	29.206707329879062
4	12.338919797021319	14.766279019186376	35.969316199444705	36.9254849843476
5	14.72240388307574	36.39273543246616	33.52370224152827	15.361158442929826
6	35.03180652990084	35.3687712524768	16.37920043438675	13.220221783235612
7	30.835315143429053	30.150812560888628	20.74574557683236	18.26812671884996
8	28.788011738668384	32.31178719201183	19.236131579282887	19.664069490036905
9	27.294572044623607	13.967188525358582	18.581194337676948	40.157045092340866
10	16.03669790823723	25.938953316138846	31.091600024899257	26.932748750724667
11	38.34306825545396	20.9000077999376	21.713601291189672	19.043322653418773
12	24.8054875972562	23.708838145580927	28.19143590428205	23.29423835288082
13	29.254505617817394	18.719082126872134	25.568851360930232	26.457560894380233
14	23.680296421861115	19.757732132744792	24.518708254733788	32.04326319066031
15	25.38331414168314	26.90119238285279	22.09475299033416	25.62074048512991
16	26.47640349653392	25.483008627323912	23.1643871989775	24.876200677164668
17	24.225125591953116	25.84287447105853	24.848790814102905	25.083209122885453
18	25.623271191220503	24.154346247772423	25.96865793436977	24.253724626637304
19	25.66673492088136	24.975792255171758	25.11200066604113	24.245472157905752
20	25.74372974918997	23.957683307332292	24.770865834633383	25.527721108844354
21	27.340836610954604	23.94703604033349	24.049039814473137	24.663087534238766
22	25.908858397349064	23.88270607665687	24.88123150219086	25.3272040238032
23	24.26034837226013	23.60562213993245	25.557874903711213	26.576154584096205
24	24.92435300630229	25.14293043776994	24.758132145584273	25.1745844103435
25	25.10814749916175	24.236591547880067	24.886414183731105	25.76884676922708
26	24.828072491598654	25.240788526164188	25.47677628420547	24.454362698031684
27	25.70792095917074	24.572712237527515	24.477605223385225	25.241761579916517
28	24.5308180289482	24.327709599948395	25.716167220939667	25.42530515016373
29	24.409125172981508	24.715597775335933	25.656242147858404	25.219034903824156
30	24.54489817959272	24.88997455989824	25.86685346741387	24.698273793095172
31	25.18054894078205	24.79721291987145	24.340599792244028	25.68163834710248
32	24.74866268447154	24.638257274606456	24.373644308571123	26.239435732350884
33	24.421201063578437	24.19205916436337	25.15430513000334	26.23243464205485
34	25.614955452577508	24.3482154778103	25.308696610975513	24.72813245863668
35	26.394997070823074	24.047529927486934	25.24545452499702	24.31201847669298
36	24.65782401048937	25.157539348269786	24.593390585819098	25.591246055421745
37	25.943518995639902	24.88540961141651	24.61786327459615	24.553208118347438
38	24.787986382526793	24.411698306516154	25.269503115233256	25.5308121957238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	123.5
2	158.0
3	158.0
4	413.0
5	668.0
6	668.0
7	800.5
8	933.0
9	920.5
10	908.0
11	908.0
12	1240.0
13	1572.0
14	2135.5
15	2699.0
16	2699.0
17	3919.5
18	5140.0
19	5140.0
20	6243.5
21	7347.0
22	8315.0
23	9283.0
24	9283.0
25	10859.0
26	12435.0
27	12435.0
28	15900.0
29	19365.0
30	23211.0
31	27057.0
32	27057.0
33	36006.5
34	44956.0
35	44956.0
36	49736.5
37	54517.0
38	62909.0
39	71301.0
40	71301.0
41	75586.5
42	79872.0
43	92000.5
44	104129.0
45	104129.0
46	106619.0
47	109109.0
48	109109.0
49	116449.0
50	123789.0
51	125033.5
52	126278.0
53	126278.0
54	120048.0
55	113818.0
56	113818.0
57	110647.5
58	107477.0
59	96676.5
60	85876.0
61	85876.0
62	82807.5
63	79739.0
64	66186.5
65	52634.0
66	52634.0
67	44128.0
68	35622.0
69	35622.0
70	28532.5
71	21443.0
72	16843.5
73	12244.0
74	12244.0
75	9117.5
76	5991.0
77	5991.0
78	6025.5
79	6060.0
80	4804.5
81	3549.0
82	3549.0
83	3570.0
84	3591.0
85	3591.0
86	2401.5
87	1212.0
88	1064.5
89	917.0
90	917.0
91	661.0
92	405.0
93	362.0
94	319.0
95	319.0
96	278.0
97	237.0
98	237.0
99	438.0
100	639.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11849603038298216
2	0.05212325387099532
3	0.01124962313762489
4	0.0026999095530299737
5	1.4999497516833185E-4
6	2.999899503366637E-4
7	7.499748758416593E-5
8	5.249824130891615E-4
9	2.2499246275249778E-4
10	3.7498743792082966E-4
11	0.0025499145778616417
12	0.003299889453703301
13	0.01567447490509068
14	0.010724640724535727
15	0.024974163365527255
16	0.01289956786447654
17	0.021824268886992286
18	0.006749773882574934
19	0.009524680923189073
20	0.007349753783248262
21	0.007049763832911598
22	0.008399718609426584
23	0.011174625650040725
24	0.015899467367843177
25	0.018374384458120653
26	0.01934935179671481
27	0.010724640724535727
28	0.007499748758416593
29	0.010724640724535727
30	0.0037498743792082965
31	0.006224791469485773
32	0.008249723634258252
33	0.010724640724535727
34	0.014399517616159858
35	0.017924399532615657
36	0.016424449780932337
37	0.011324620625209055
38	0.0068247713701591
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1333378.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.662619336610746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09630025556395	40.07225750781904
2	12.560000606669869	12.726450592066312
3	3.8307382255463014	5.822256974971679
4	1.5358894700262609	3.1124873425219706
5	0.775215248220022	1.9637217512253593
6	0.46197412923467407	1.4042891671667106
7	0.30370243977117267	1.0770452768408811
8	0.23337917115328252	0.9458880087385985
9	0.17312206114030315	0.7893735374088569
>10	0.818269934472083	7.55470024318307
>50	0.08934696844266264	3.2184580164479053
>100	0.10025950946778363	11.122775905346858
>500	0.015276217483308345	5.219164996595003
>1k	0.006525762808403565	4.971130679667849
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2843	0.21321785720178374	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2431	0.18231889231710738	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2272	0.17039429179122498	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2044	0.15329486462203518	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2021	0.15156992240759934	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1984	0.1487950153669852	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1969	0.14767005305322273	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1956	0.14669508571462855	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1946	0.14594511083878692	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1871	0.14032029926997447	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1833	0.13747039474177616	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1787	0.13402051031290452	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1683	0.12622077160415127	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1633	0.12247089722494295	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1589	0.11917100777123965	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1560	0.11699608063129885	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1557	0.11677108816854635	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1533	0.11497114846652637	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1465	0.1098713193108031	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1461	0.10957132936046643	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1433	0.10747139970810977	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1421	0.10657142985709979	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1393	0.10447150020474313	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1389	0.10417151025440648	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1356	0.101696593164129	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1346	0.10094661828828734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.499748758416593E-5	0.0
9	0.0	0.0	0.0	1.4999497516833185E-4	0.0
10	0.0	0.0	0.0	1.4999497516833185E-4	0.0
11	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
12	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
13	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
14	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
15	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
16	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
17	0.0	0.0	0.0	1.4999497516833185E-4	7.499748758416593E-5
18	0.0	0.0	0.0	2.2499246275249778E-4	7.499748758416593E-5
19	0.0	0.0	0.0	2.2499246275249778E-4	7.499748758416593E-5
20	7.499748758416593E-5	0.0	0.0	2.2499246275249778E-4	7.499748758416593E-5
21	7.499748758416593E-5	0.0	0.0	5.249824130891615E-4	7.499748758416593E-5
22	7.499748758416593E-5	0.0	0.0	5.249824130891615E-4	7.499748758416593E-5
23	7.499748758416593E-5	0.0	0.0	0.001049964826178323	7.499748758416593E-5
24	7.499748758416593E-5	0.0	0.0	0.0017999397020199823	7.499748758416593E-5
25	7.499748758416593E-5	0.0	0.0	0.0023999196026933097	7.499748758416593E-5
26	7.499748758416593E-5	0.0	0.0	0.0028499045281983053	7.499748758416593E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	490	0.0	20.569595	28
CTAGCGG	485	0.0	20.45332	29
TAGCGGC	510	0.0	18.823265	30
ATCGTTT	355	0.0	18.478613	29
AAGACGG	515	0.0	18.32709	5
ACTATAT	70	1.7884024E-5	18.284771	8
GTTCGGA	55	0.0013546696	17.473963	1
CGCTTCG	350	0.0	17.371183	32
AGCGGCG	575	0.0	17.251932	31
CTATACA	130	2.6011548E-10	17.227942	4
CGTTTAT	375	0.0	17.066427	31
CAAGACG	545	0.0	17.024729	4
GTAATAC	85	4.9875853E-6	16.940302	3
AAACGCT	380	0.0	16.84187	29
ACCGTCG	430	0.0	16.743322	8
CGCAAGA	590	0.0	16.276451	2
CATCGTT	395	0.0	16.20109	28
GTATTAG	440	0.0	16.0178	1
CTATTAG	70	3.6657855E-4	16.0178	1
TCTATAC	80	5.6244182E-5	15.999176	3
>>END_MODULE