FastQCFastQC Report
Thu 2 Feb 2017
SRR4062964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333378
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31980.23984196529416263No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24860.1864437541342365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23630.17721906316138408No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22560.16919433198987835No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21450.16086961086803592No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21380.16034462845494676No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20310.152319897283441No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20240.15179491487035185No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT19650.14737006310288606No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19540.14654509073946023No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19320.14489514601260858No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17650.13237056558605287No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17550.1316205907102112No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17060.12794571381858708No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16910.1268207515048246No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16360.12269588968769546No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15670.11752106304438802No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15510.11632110324304136No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT15330.11497114846652637No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15040.11279622132658557No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14720.11039630172389224No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14450.10837136955911977No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT14320.1073964022205256No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14260.10694641729502062No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14010.10507148010541648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13730.1029715504530598No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13650.10237157055238649No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13630.10222157557721816No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13440.100796623313119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA400.00448458119.99943712
GGATCGA507.1926793E-419.201626
GATATAC1003.261448E-919.201621
TCGACAT507.1981264E-419.1994613
TCTAGCG4950.019.07017128
ATCGTTT4300.018.9762129
TAGCGGC5150.018.64025330
CATCGTT4400.018.54493128
CTAGCGG5100.018.50928329
ACCGTCG3550.018.029698
TACCGTC3500.017.8300767
TAGGACG753.2330172E-517.0674674
GGACCGT854.9811333E-616.9426066
AACCGCG2750.016.8741517
CGTTTAT4900.016.65259431
CGCGGTC2800.016.57158510
TCGTTTA4950.016.48438530
CGTCGTA4150.016.19292310
GCATCGT5150.016.15488627
AGCGGCG5950.016.13399931
Produced by FastQC (version 0.11.5)