##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1333378
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174011420617408	32.0	32.0	32.0	32.0	32.0
2	31.23160049138354	32.0	32.0	32.0	32.0	32.0
3	31.341402813005764	32.0	32.0	32.0	32.0	32.0
4	31.45143087706562	32.0	32.0	32.0	32.0	32.0
5	31.35258568837944	32.0	32.0	32.0	32.0	32.0
6	34.881011986098464	36.0	36.0	36.0	36.0	36.0
7	34.91234743636088	36.0	36.0	36.0	36.0	36.0
8	34.84289526300869	36.0	36.0	36.0	32.0	36.0
9	34.97426986195963	36.0	36.0	36.0	36.0	36.0
10	34.80704946384296	36.0	36.0	36.0	32.0	36.0
11	34.989720844351716	36.0	36.0	36.0	36.0	36.0
12	34.880434505444065	36.0	36.0	36.0	32.0	36.0
13	34.93890329673956	36.0	36.0	36.0	36.0	36.0
14	34.88484210778939	36.0	36.0	36.0	32.0	36.0
15	34.847816598143964	36.0	36.0	36.0	32.0	36.0
16	34.865935241169424	36.0	36.0	36.0	32.0	36.0
17	34.82080625299052	36.0	36.0	36.0	32.0	36.0
18	34.83144239667971	36.0	36.0	36.0	32.0	36.0
19	34.82746228001362	36.0	36.0	36.0	32.0	36.0
20	34.808478915956314	36.0	36.0	36.0	32.0	36.0
21	34.80883440404747	36.0	36.0	36.0	32.0	36.0
22	34.7862984090033	36.0	36.0	36.0	32.0	36.0
23	34.71889591698678	36.0	36.0	36.0	32.0	36.0
24	34.698774091067946	36.0	36.0	36.0	32.0	36.0
25	34.66418899966851	36.0	36.0	36.0	32.0	36.0
26	34.60363602819306	36.0	36.0	36.0	32.0	36.0
27	34.58884652364146	36.0	36.0	36.0	32.0	36.0
28	34.56141544258267	36.0	36.0	36.0	32.0	36.0
29	34.518270887925254	36.0	36.0	36.0	32.0	36.0
30	34.49789932037277	36.0	36.0	36.0	32.0	36.0
31	34.50394936769618	36.0	36.0	36.0	32.0	36.0
32	34.47584630914864	36.0	36.0	36.0	32.0	36.0
33	34.43234401647545	36.0	36.0	36.0	32.0	36.0
34	34.42378830309185	36.0	36.0	36.0	32.0	36.0
35	34.38615231389748	36.0	36.0	36.0	32.0	36.0
36	34.35377964838178	36.0	36.0	36.0	32.0	36.0
37	34.34621390183429	36.0	36.0	36.0	32.0	36.0
38	33.89442228685339	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	20.0
22	92.0
23	320.0
24	827.0
25	2136.0
26	4547.0
27	8844.0
28	15494.0
29	25238.0
30	38246.0
31	55549.0
32	80850.0
33	130720.0
34	313321.0
35	657169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25400932747104	18.468857677312705	11.825622193920793	26.451510801295463
2	16.118685024051693	20.40179153998341	36.24426081726262	27.235262618702272
3	17.91487485169247	24.21796369821611	28.74916190307625	29.117999547015177
4	11.797131339645636	15.620211868379114	36.15089528452236	36.43176150745289
5	14.218259352006221	36.84524331828133	33.17336357234301	15.763133757369447
6	35.03680126715755	35.891322640691534	15.987589415754572	13.084286676396339
7	30.57120722020686	30.46058517934645	20.643197794459	18.32500980598769
8	28.682149031195976	32.607689682715275	18.977383947890456	19.73277733819829
9	27.27079089553627	13.76754637282461	18.3245032715229	40.63715946011622
10	15.778010772768386	26.223711190209407	31.042282590924298	26.95599544609791
11	38.46894624459149	20.90050603119888	21.572514263299723	19.058033460909897
12	24.68240367488516	23.792031499015657	28.073047717258838	23.452517108840347
13	29.344963952430557	18.748485987083924	25.42731725535989	26.47923280512563
14	23.6213763999229	19.63630691094867	24.495772763441998	32.24654392568643
15	25.390024434181456	26.988670879525532	21.96586414355119	25.655440542741818
16	26.45626371516554	25.565218565178068	23.0040543641788	24.974463355477592
17	24.22988829874199	25.925506495532403	24.65939891013651	25.185206295589097
18	25.766736814316722	24.124591826173823	25.896407470349743	24.212263889159715
19	25.75503720625359	24.9692135313467	25.101959084370673	24.173790178029037
20	25.828984729011577	23.799852704934384	24.75427073193048	25.616891834123557
21	27.39165292336676	23.994639175567002	23.954815479793037	24.658892421273205
22	25.792030311007313	24.00828872854414	24.828311872786166	25.371369087662377
23	24.204893597075092	23.525114840161248	25.582225555451394	26.68776600731227
24	24.846742634121757	25.078634865731996	24.832493111480765	25.24212938866548
25	25.071772703273492	24.19910062877988	24.94757667754632	25.78154999040031
26	24.879666531171203	25.158507190009132	25.404723941747953	24.557102337071708
27	25.77195029759048	24.435418066621867	24.46046726504752	25.332164370740134
28	24.65069920157675	24.145291132747055	25.842034291851224	25.361975373824976
29	24.597150995441652	24.577576651182184	25.679814726206672	25.14545762716949
30	24.58432642506476	24.7257716866485	25.9695300207443	24.720371867542436
31	25.271153416360555	24.591151196434918	24.423081826758803	25.714613560445727
32	24.858667234647637	24.477155015306987	24.48532974145366	26.17884800859171
33	24.419181957404426	24.034294851122485	25.251354079638332	26.295169111834753
34	25.504095612796974	24.1990643313449	25.438022826235322	24.858817229622808
35	26.49023757704117	24.001595946535794	25.157832212620878	24.35033426380216
36	24.709497231842732	25.02726158673684	24.725171706747822	25.538069474672596
37	26.122599892903587	24.683773093601364	24.617175324626626	24.576451688868424
38	24.8460676567335	24.161865577503153	25.377574851242485	25.614491914520865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	63.5
2	81.0
3	81.0
4	321.5
5	562.0
6	562.0
7	730.0
8	898.0
9	959.0
10	1020.0
11	1020.0
12	1253.5
13	1487.0
14	2104.0
15	2721.0
16	2721.0
17	4074.0
18	5427.0
19	5427.0
20	6511.5
21	7596.0
22	8457.5
23	9319.0
24	9319.0
25	10799.0
26	12279.0
27	12279.0
28	15910.0
29	19541.0
30	23392.5
31	27244.0
32	27244.0
33	35976.5
34	44709.0
35	44709.0
36	49534.0
37	54359.0
38	62611.0
39	70863.0
40	70863.0
41	75467.0
42	80071.0
43	91899.5
44	103728.0
45	103728.0
46	106618.0
47	109508.0
48	109508.0
49	116804.5
50	124101.0
51	125234.0
52	126367.0
53	126367.0
54	119915.5
55	113464.0
56	113464.0
57	110977.0
58	108490.0
59	97270.0
60	86050.0
61	86050.0
62	83124.0
63	80198.0
64	66674.0
65	53150.0
66	53150.0
67	44442.0
68	35734.0
69	35734.0
70	28939.0
71	22144.0
72	17059.0
73	11974.0
74	11974.0
75	8931.0
76	5888.0
77	5888.0
78	5991.0
79	6094.0
80	4763.5
81	3433.0
82	3433.0
83	3303.5
84	3174.0
85	3174.0
86	2015.5
87	857.0
88	727.0
89	597.0
90	597.0
91	358.5
92	120.0
93	81.5
94	43.0
95	43.0
96	30.0
97	17.0
98	17.0
99	20.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008399718609426584
2	0.0
3	0.0
4	2.2499246275249778E-4
5	7.499748758416593E-5
6	0.0
7	6.749773882574934E-4
8	0.0024749170902774757
9	0.00404986432954496
10	0.0011999598013466548
11	0.004574846742634122
12	2.2499246275249778E-4
13	7.499748758416593E-5
14	7.499748758416593E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	7.499748758416593E-5
22	7.499748758416593E-5
23	2.2499246275249778E-4
24	0.0
25	1.4999497516833185E-4
26	0.0
27	1.4999497516833185E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1333378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.63029984947679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36942731538768	37.62498449529603
2	13.75045589609748	13.373775865884529
3	3.974940163816951	5.799075961504401
4	1.673628916048281	3.255563040967309
5	0.8854071295498619	2.152880709943716
6	0.5014220799593663	1.4630583659743388
7	0.31686410499108214	1.0786437506076694
8	0.23321126606910245	0.9072907037773251
9	0.17899389667829166	0.7834074180022431
>10	0.8900941744363394	7.8263142024742045
>50	0.09236156283966426	3.1643917548384235
>100	0.10949745044200418	11.604650944762458
>500	0.01657174296845753	5.568616495716185
>1k	0.00712430071541165	5.397346290251207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3198	0.23984196529416263	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2486	0.1864437541342365	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2363	0.17721906316138408	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2256	0.16919433198987835	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2145	0.16086961086803592	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2138	0.16034462845494676	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2031	0.152319897283441	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2024	0.15179491487035185	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1965	0.14737006310288606	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1954	0.14654509073946023	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1932	0.14489514601260858	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1765	0.13237056558605287	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1755	0.1316205907102112	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1706	0.12794571381858708	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1691	0.1268207515048246	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1636	0.12269588968769546	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1567	0.11752106304438802	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1551	0.11632110324304136	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1533	0.11497114846652637	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1504	0.11279622132658557	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1472	0.11039630172389224	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1445	0.10837136955911977	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1432	0.1073964022205256	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1426	0.10694641729502062	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1401	0.10507148010541648	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1373	0.1029715504530598	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1365	0.10237157055238649	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1363	0.10222157557721816	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1344	0.100796623313119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	7.499748758416593E-5
14	0.0	0.0	0.0	0.0	7.499748758416593E-5
15	0.0	0.0	0.0	0.0	7.499748758416593E-5
16	0.0	0.0	0.0	0.0	1.4999497516833185E-4
17	0.0	0.0	0.0	0.0	1.4999497516833185E-4
18	0.0	0.0	0.0	0.0	1.4999497516833185E-4
19	0.0	0.0	0.0	0.0	1.4999497516833185E-4
20	0.0	0.0	0.0	7.499748758416593E-5	1.4999497516833185E-4
21	0.0	0.0	0.0	3.7498743792082966E-4	1.4999497516833185E-4
22	0.0	0.0	0.0	3.7498743792082966E-4	1.4999497516833185E-4
23	0.0	0.0	0.0	8.999698510099911E-4	1.4999497516833185E-4
24	0.0	0.0	0.0	0.0016499447268516505	1.4999497516833185E-4
25	0.0	0.0	0.0	0.002249924627524978	1.4999497516833185E-4
26	0.0	0.0	0.0	0.0026999095530299733	1.4999497516833185E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	40	0.004484581	19.999437	12
GGATCGA	50	7.1926793E-4	19.20162	6
GATATAC	100	3.261448E-9	19.20162	1
TCGACAT	50	7.1981264E-4	19.19946	13
TCTAGCG	495	0.0	19.070171	28
ATCGTTT	430	0.0	18.97621	29
TAGCGGC	515	0.0	18.640253	30
CATCGTT	440	0.0	18.544931	28
CTAGCGG	510	0.0	18.509283	29
ACCGTCG	355	0.0	18.02969	8
TACCGTC	350	0.0	17.830076	7
TAGGACG	75	3.2330172E-5	17.067467	4
GGACCGT	85	4.9811333E-6	16.942606	6
AACCGCG	275	0.0	16.874151	7
CGTTTAT	490	0.0	16.652594	31
CGCGGTC	280	0.0	16.571585	10
TCGTTTA	495	0.0	16.484385	30
CGTCGTA	415	0.0	16.192923	10
GCATCGT	515	0.0	16.154886	27
AGCGGCG	595	0.0	16.133999	31
>>END_MODULE