FastQCFastQC Report
Thu 2 Feb 2017
SRR4062963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380010
Sequences flagged as poor quality0
Sequence length38
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT172564.540933133338596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT119923.1557064287781897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT70191.847056656403779No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA60701.597326386147733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56381.483645167232441No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA53391.4049630272887554No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC45541.1983895160653668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44311.166021946790874No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA36230.9533959632641247No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA29880.7862950974974343No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA26790.7049814478566354No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.6197205336701666No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23210.6107734006999815No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG19800.5210389200284203No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA19320.5084076734822768No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.5076182205731428No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT19100.5026183521486277No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG16880.4441988368727139No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA14000.36841135759585275No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC13310.3502539406857714No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA11680.30736033262282575No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG11490.3023604641983106No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA11160.29367648219783693No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA10900.2868345569853425No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA9730.25604589352911766No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG9330.24551985474066473No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC9190.24183574116470619No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA9190.24183574116470619No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC9000.23683587274019105No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA8380.220520512618089No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG8310.21867845583010975No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA8250.21709955001184178No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8060.21209968158732664No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7850.20657351122338885No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7660.20157364279887371No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7170.18867924528301888No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA7060.18578458461619432No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7010.1844688297676377No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6850.18025841425225653No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA6680.17578484776716402No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA6560.17262703613062813No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT6550.17236388516091683No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA6430.16920607352438094No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6230.16394305413015448No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6180.16262729928159786No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT6170.16236414831188653No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6120.16104839346332991No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC5970.15710112891766007No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA5880.15473277019025813No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT5650.1486802978868977No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5500.14473303334122786No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG5470.14394358043209388No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA5320.13999631588642406No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT5290.13920686297729007No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA5230.13762795715902212No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT5110.13447014552248626No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT5040.132628088734507No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4990.13131233388595037No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC4950.13025973000710508No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA4800.12631246546143524No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA4780.12578616352201258No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA4470.11762848346096155No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4360.11473382279413699No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA4230.1113128601878898No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA4220.11104970921817847No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4050.10657614273308597No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT4050.10657614273308597No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG4050.10657614273308597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATTC302.2406512E-532.00355514
TTAAGAC200.003758016331.9867083
TCTAATT453.8401413E-728.44760312
GAACACT453.8489998E-728.4401136
TCTGGGG356.4376916E-527.417183
AACACTC557.5820935E-826.1778347
AACGCTT451.234169E-524.89165330
TATTCCA451.2379542E-524.8818265
ACTCATT401.5896154E-424.00266613
TCCTATT401.5953042E-423.990032
CACTCTA552.2098957E-623.2753129
CTCTAAT552.2098957E-623.27531211
TTGAACA552.2200384E-623.263064
TATAGCA350.002067419222.8506575
TAAGGCG350.002067419222.8506575
GTCCTAT502.7775197E-522.4142931
AAACGCT502.7888489E-522.40248929
ACACTCT653.8081816E-722.1504758
ACTCTAA604.7023022E-621.33570310
GTAAACG604.707672E-621.33289327
Produced by FastQC (version 0.11.5)