##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062963_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380010
Sequences flagged as poor quality	0
Sequence length	38
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.691973895423803	32.0	32.0	32.0	14.0	32.0
2	29.605976158522143	32.0	32.0	32.0	14.0	32.0
3	29.98865293018605	32.0	32.0	32.0	21.0	32.0
4	29.73498855293282	32.0	32.0	32.0	21.0	32.0
5	30.035164864082525	32.0	32.0	32.0	21.0	32.0
6	33.311389173969104	36.0	36.0	36.0	21.0	36.0
7	32.7556932712297	36.0	36.0	36.0	14.0	36.0
8	32.679163706218255	36.0	36.0	36.0	14.0	36.0
9	32.46480882082051	36.0	32.0	36.0	14.0	36.0
10	32.61092602826241	36.0	32.0	36.0	14.0	36.0
11	33.15972474408568	36.0	36.0	36.0	21.0	36.0
12	32.97480066314044	36.0	36.0	36.0	21.0	36.0
13	33.01534170153417	36.0	36.0	36.0	21.0	36.0
14	32.956614299623695	36.0	36.0	36.0	21.0	36.0
15	33.091355490644986	36.0	36.0	36.0	21.0	36.0
16	32.92529143969895	36.0	36.0	36.0	21.0	36.0
17	32.80857872161259	36.0	36.0	36.0	14.0	36.0
18	32.910849714481195	36.0	36.0	36.0	21.0	36.0
19	32.830301834162256	36.0	36.0	36.0	14.0	36.0
20	32.51017604799873	36.0	36.0	36.0	14.0	36.0
21	32.3725586168785	36.0	32.0	36.0	14.0	36.0
22	32.45432225467751	36.0	32.0	36.0	14.0	36.0
23	32.61304702507829	36.0	36.0	36.0	14.0	36.0
24	32.687187179284756	36.0	36.0	36.0	14.0	36.0
25	32.67845583010973	36.0	36.0	36.0	14.0	36.0
26	32.68538196363254	36.0	36.0	36.0	14.0	36.0
27	32.751580221573114	36.0	36.0	36.0	14.0	36.0
28	32.726551932843876	36.0	36.0	36.0	14.0	36.0
29	32.657259019499485	36.0	36.0	36.0	14.0	36.0
30	32.524988816083784	36.0	36.0	36.0	14.0	36.0
31	32.52107312965448	36.0	36.0	36.0	14.0	36.0
32	32.361798373727005	36.0	36.0	36.0	14.0	36.0
33	32.12114154890661	36.0	32.0	36.0	14.0	36.0
34	31.88657140601563	36.0	32.0	36.0	14.0	36.0
35	31.699276334833293	36.0	32.0	36.0	14.0	36.0
36	31.6218783716218	36.0	32.0	36.0	14.0	36.0
37	31.554509091866002	36.0	32.0	36.0	14.0	36.0
38	30.633138601615748	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	1.0
9	1.0
10	1.0
11	2.0
12	3.0
13	4.0
14	813.0
15	1175.0
16	1244.0
17	1379.0
18	1671.0
19	1942.0
20	2339.0
21	2852.0
22	3706.0
23	4718.0
24	5345.0
25	6521.0
26	8049.0
27	10275.0
28	13042.0
29	16582.0
30	20942.0
31	25620.0
32	31908.0
33	41639.0
34	69208.0
35	109025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.53156245965607	16.88767458220151	14.761203428279352	13.819559529863074
2	12.1320944976423	25.901811125825724	41.89943104478712	20.06666333174486
3	18.131143657968497	40.339768136538886	26.676667587804143	14.852420617688471
4	9.475049868683518	19.066152979752523	32.32911752167117	39.12967962989279
5	9.057919528433462	45.480118944238306	27.01481539959475	18.44714612773348
6	23.544705520132418	36.74439289595773	22.23263133241581	17.478270251494045
7	23.473066498250045	38.66477197968475	21.014710139206862	16.847451382858345
8	33.438331399158436	25.78741970542648	20.07068290847274	20.703565986942348
9	32.97649780135629	14.51052217459152	20.118313610012446	32.39466641403974
10	18.96570326336094	30.721276187017608	26.9302933893323	23.38272716028915
11	29.50434862699175	28.657254509809082	20.863672846409916	20.974724016789253
12	27.804663671937707	31.13702332362283	27.452296178167952	13.60601682627151
13	32.49725621070196	25.547902481648855	27.49368994259756	14.461151365051625
14	27.786097213705403	22.03559782823245	32.14647587816837	18.03182907989378
15	22.95136338869015	26.19406737053796	37.004835495750186	13.849733745021704
16	16.74351645690885	30.978887362420455	32.121633215953175	20.155962964717524
17	15.546231758933649	28.553191265345657	31.860536124155068	24.040040851565625
18	17.431168516734825	25.918849641828913	39.09249288131668	17.55748896011958
19	17.989894204958155	28.912837517764096	39.60708458339913	13.490183693878624
20	23.387625235865812	20.40012316340468	41.03341465276078	15.178836947968724
21	25.842267873026902	23.186643718452785	34.29021069197286	16.680877716547453
22	22.30336812518751	25.06487285972198	34.98265670809055	17.649102306999954
23	17.839970313211413	27.882158187421542	36.471325141393855	17.806546357973193
24	16.583321928263867	31.34771073936433	36.81556423510586	15.253403097265942
25	19.49007335426663	27.614841407917627	36.058609716874116	16.83647552094163
26	16.338665059340347	28.455729653328138	38.18891523898012	17.016690048351393
27	17.79224094597191	30.805407809584466	37.48016285539084	13.922188389052792
28	15.976778275160598	29.232425149149055	38.50216716799697	16.28862940769338
29	13.973018780528887	31.663455869968	40.08811268317332	14.275412666329796
30	16.434736842105263	30.975526315789477	38.25394736842105	14.33578947368421
31	14.05228586165879	32.5802393759803	36.34404244344559	17.023432318915336
32	13.232047034178205	34.000036844141384	36.18647346300717	16.581442658673243
33	13.86560482565683	33.2198153007219	35.68370051241022	17.230879361211045
34	13.900637170656987	33.91953384689481	36.5455928371596	15.634236145288597
35	15.987008680180868	33.73505708705974	35.404241655393136	14.873692577366256
36	15.2676336589102	36.47355773659594	34.23215709807575	14.026651506418103
37	15.391336616074556	37.609581884742624	34.274555421918215	12.724526077264606
38	14.345942702549069	38.26760214957551	32.73997189340548	14.646483254469949
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5193.0
1	3672.5
2	2152.0
3	2152.0
4	4271.5
5	6391.0
6	6391.0
7	7019.5
8	7648.0
9	7085.0
10	6522.0
11	6522.0
12	8018.0
13	9514.0
14	10655.0
15	11796.0
16	11796.0
17	16187.0
18	20578.0
19	20578.0
20	22018.5
21	23459.0
22	18075.0
23	12691.0
24	12691.0
25	9769.5
26	6848.0
27	6848.0
28	9261.0
29	11674.0
30	14379.5
31	17085.0
32	17085.0
33	13747.5
34	10410.0
35	10410.0
36	9992.5
37	9575.0
38	11573.0
39	13571.0
40	13571.0
41	16966.5
42	20362.0
43	24973.0
44	29584.0
45	29584.0
46	40036.5
47	50489.0
48	50489.0
49	43420.5
50	36352.0
51	27253.0
52	18154.0
53	18154.0
54	15000.5
55	11847.0
56	11847.0
57	11377.5
58	10908.0
59	9636.0
60	8364.0
61	8364.0
62	7626.0
63	6888.0
64	5650.0
65	4412.0
66	4412.0
67	3658.0
68	2904.0
69	2904.0
70	2356.5
71	1809.0
72	1406.0
73	1003.0
74	1003.0
75	756.5
76	510.0
77	510.0
78	463.0
79	416.0
80	333.5
81	251.0
82	251.0
83	259.0
84	267.0
85	267.0
86	174.0
87	81.0
88	73.0
89	65.0
90	65.0
91	46.5
92	28.0
93	29.0
94	30.0
95	30.0
96	25.0
97	20.0
98	20.0
99	89.5
100	159.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12131259703692009
2	0.050261835214862764
3	0.011841793637009553
4	0.0031578116365358804
5	0.0
6	2.631509697113234E-4
7	0.0
8	7.894529091339701E-4
9	7.894529091339701E-4
10	7.894529091339701E-4
11	0.0013157548485566168
12	0.0023683587274019105
13	0.016052209152390726
14	0.011315491697586905
15	0.0292097576379569
16	0.012631246546143522
17	0.025788795031709694
18	0.006315623273071761
19	0.007894529091339702
20	0.007105076182205732
21	0.006315623273071761
22	0.009473434909607642
23	0.011315491697586905
24	0.01631536012210205
25	0.01921002078892661
26	0.02184153048603984
27	0.010789189758164258
28	0.005526170363937791
29	0.011052340727875582
30	0.0026315096971132336
31	0.0057893213336491145
32	0.008157680061051026
33	0.010789189758164258
34	0.012894397515854847
35	0.0168416620615247
36	0.01815741691008131
37	0.011315491697586905
38	0.007368227151917055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	380010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.65901091420378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91396552270015	27.004209814201612
2	11.923686042191289	8.265263293476943
3	4.0347863113696105	4.19525108406718
4	1.8217556338565093	2.525609935873799
5	0.995102836173386	1.724464002984426
6	0.628988194739763	1.3080065223834467
7	0.41205551705612564	0.9997005664034297
8	0.3173286898570517	0.8798638820116432
9	0.23779719506149313	0.7417634021103001
>10	1.3926804909783346	9.255609320855255
>50	0.14767398911336116	3.6234474819411915
>100	0.13101333393134093	9.082372561942128
>500	0.02499098277303035	5.995421034588968
>1k	0.013631445148925645	10.405501772841479
>5k	0.003029210033094588	6.316688670800197
>10k+	0.001514605016547294	7.676826653518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	17256	4.540933133338596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	11992	3.1557064287781897	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	7019	1.847056656403779	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	6070	1.597326386147733	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5638	1.483645167232441	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	5339	1.4049630272887554	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	4554	1.1983895160653668	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4431	1.166021946790874	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	3623	0.9533959632641247	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	2988	0.7862950974974343	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	2679	0.7049814478566354	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2355	0.6197205336701666	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.6107734006999815	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1980	0.5210389200284203	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1932	0.5084076734822768	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1929	0.5076182205731428	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1910	0.5026183521486277	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1688	0.4441988368727139	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1400	0.36841135759585275	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1331	0.3502539406857714	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	1168	0.30736033262282575	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	1149	0.3023604641983106	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1116	0.29367648219783693	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	1090	0.2868345569853425	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	973	0.25604589352911766	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	933	0.24551985474066473	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	919	0.24183574116470619	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	919	0.24183574116470619	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	900	0.23683587274019105	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	838	0.220520512618089	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	831	0.21867845583010975	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	825	0.21709955001184178	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	806	0.21209968158732664	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	785	0.20657351122338885	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	766	0.20157364279887371	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	717	0.18867924528301888	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	706	0.18578458461619432	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	701	0.1844688297676377	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	685	0.18025841425225653	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	668	0.17578484776716402	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	656	0.17262703613062813	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	655	0.17236388516091683	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	643	0.16920607352438094	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	623	0.16394305413015448	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.16262729928159786	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	617	0.16236414831188653	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	612	0.16104839346332991	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	597	0.15710112891766007	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	588	0.15473277019025813	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	565	0.1486802978868977	No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	550	0.14473303334122786	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	547	0.14394358043209388	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	532	0.13999631588642406	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	529	0.13920686297729007	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	523	0.13762795715902212	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	511	0.13447014552248626	No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT	504	0.132628088734507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	499	0.13131233388595037	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	495	0.13025973000710508	No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA	480	0.12631246546143524	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	478	0.12578616352201258	No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA	447	0.11762848346096155	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	436	0.11473382279413699	No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA	423	0.1113128601878898	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA	422	0.11104970921817847	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	405	0.10657614273308597	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	405	0.10657614273308597	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	405	0.10657614273308597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.6315096971132336E-4	0.0
6	0.0	0.0	0.0	2.6315096971132336E-4	0.0
7	0.0	0.0	0.0	2.6315096971132336E-4	0.0
8	0.0	0.0	0.0	2.6315096971132336E-4	0.0
9	0.0	0.0	0.0	2.6315096971132336E-4	0.0
10	2.6315096971132336E-4	0.0	0.0	2.6315096971132336E-4	0.0
11	2.6315096971132336E-4	0.0	0.0	2.6315096971132336E-4	0.0
12	2.6315096971132336E-4	0.0	0.0	2.6315096971132336E-4	0.0
13	2.6315096971132336E-4	0.0	0.0	2.6315096971132336E-4	0.0
14	2.6315096971132336E-4	0.0	0.0	2.6315096971132336E-4	0.0
15	2.6315096971132336E-4	0.0	0.0	2.6315096971132336E-4	0.0
16	2.6315096971132336E-4	0.0	0.0	5.263019394226467E-4	0.0
17	2.6315096971132336E-4	0.0	0.0	5.263019394226467E-4	0.0
18	2.6315096971132336E-4	0.0	0.0	0.0013157548485566168	0.0
19	2.6315096971132336E-4	0.0	0.0	0.0013157548485566168	0.0
20	2.6315096971132336E-4	0.0	0.0	0.0018420567879792638	0.0
21	2.6315096971132336E-4	0.0	0.0	0.0028946606668245572	0.0
22	2.6315096971132336E-4	0.0	0.0	0.006841925212494408	0.0
23	2.6315096971132336E-4	0.0	0.0	0.01947317175863793	0.0
24	2.6315096971132336E-4	0.0	0.0	0.037630588668719246	0.0
25	2.6315096971132336E-4	0.0	0.0	0.04473566485092498	0.0
26	2.6315096971132336E-4	0.0	0.0	0.06210362885187232	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCATTC	30	2.2406512E-5	32.003555	14
TTAAGAC	20	0.0037580163	31.986708	3
TCTAATT	45	3.8401413E-7	28.447603	12
GAACACT	45	3.8489998E-7	28.440113	6
TCTGGGG	35	6.4376916E-5	27.41718	3
AACACTC	55	7.5820935E-8	26.177834	7
AACGCTT	45	1.234169E-5	24.891653	30
TATTCCA	45	1.2379542E-5	24.881826	5
ACTCATT	40	1.5896154E-4	24.002666	13
TCCTATT	40	1.5953042E-4	23.99003	2
CACTCTA	55	2.2098957E-6	23.275312	9
CTCTAAT	55	2.2098957E-6	23.275312	11
TTGAACA	55	2.2200384E-6	23.26306	4
TATAGCA	35	0.0020674192	22.850657	5
TAAGGCG	35	0.0020674192	22.850657	5
GTCCTAT	50	2.7775197E-5	22.414293	1
AAACGCT	50	2.7888489E-5	22.402489	29
ACACTCT	65	3.8081816E-7	22.150475	8
ACTCTAA	60	4.7023022E-6	21.335703	10
GTAAACG	60	4.707672E-6	21.332893	27
>>END_MODULE