FastQCFastQC Report
Thu 2 Feb 2017
SRR4062963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380010
Sequences flagged as poor quality0
Sequence length38
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT222695.8601089445014605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT162464.27515065393016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT103632.7270334991184444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71741.8878450567090341No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA54781.4415410120786296No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC52081.3704902502565721No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA49151.2933870161311545No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35620.9373437541117339No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35050.9223441488381884No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.8102418357411647No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA28640.7536643772532302No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA25950.6828767664008842No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT25180.6626141417331123No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA24790.6523512539143707No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA22430.5902476250624984No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG21910.5765637746375095No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17790.46814557511644433No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA16040.42209415541696277No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC14770.38867398226362465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14180.37314807505065656No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA13720.3610431304439357No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA13330.3507802426251941No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG12300.32367569274492775No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC11570.30446567195600116No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA11310.2976237467435067No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC11300.2973605957737954No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11170.29393963316754823No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11140.2931501802584142No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.283150443409384No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA10320.27157180074208576No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG10010.2634141206810347No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA9210.24236204310412882No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9130.24025683534643827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG8870.23341491013394383No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8780.23104655140654193No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8680.2284150417094287No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA8430.22183626746664561No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC8070.212362832557038No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT8020.21104707770848138No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7980.20999447382963604No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA7740.20367885055656432No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7620.20052103892002843No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA7080.18631088655561695No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7070.18604773558590562No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7040.18525828267677166No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA7040.18525828267677166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT6860.18052156522196786No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6850.18025841425225653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT6570.17289018710033946No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA6530.17183758322149417No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6370.167627167706113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT6310.16604826188784508No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA6140.16157469540275254No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA6080.15999578958448463No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT6040.1589431857056393No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5500.14473303334122786No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA5430.14289097655324862No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG5220.13736480618931082No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC5180.1363122023104655No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC5000.13157548485566167No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5000.13157548485566167No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA4920.1294702770979711No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA4830.1271019183705692No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT4820.12683876740085787No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT4750.12499671061287862No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT4710.12394410673403332No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4660.1226283518854767No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA4530.1192073892792295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT4410.11604957764269361No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG4410.11604957764269361No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3900.10262887818741612No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA3860.10157627430857083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCTC200.003751135231.99868419
CGACCAT308.408466E-426.66557110
CCCTCCA350.00206450522.85620331
AACATGG502.7892975E-522.4020272
GAACATG400.00446208820.0123441
TAGTTCC400.004475708620.001814
ATACATG902.0132575E-819.5676251
CGTTAAA450.00880830917.788751
CTACATG450.00880830917.788751
TTACGCG450.008835074517.7793875
GGACAGC450.008835074517.7793876
CCAATTA450.00884177517.77704628
GTCACTA450.00884177517.77704621
CAAGACA550.001361542617.4561254
AAAGGAT550.001361542617.4561255
GTTCAAA600.002427848316.0098741
TACCCAC600.002438539415.99934330
GCAAATT600.002438539415.99934324
CGCGCAA600.002438539415.99934321
CGCAAAT600.002438539415.99934323
Produced by FastQC (version 0.11.5)