##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380010
Sequences flagged as poor quality	0
Sequence length	38
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.71568642930449	32.0	32.0	32.0	14.0	32.0
2	31.14288045051446	32.0	32.0	32.0	32.0	32.0
3	31.302820978395307	32.0	32.0	32.0	32.0	32.0
4	31.502694665929845	32.0	32.0	32.0	32.0	32.0
5	31.26496934291203	32.0	32.0	32.0	32.0	32.0
6	34.6563406226152	36.0	36.0	36.0	32.0	36.0
7	34.74933817531117	36.0	36.0	36.0	32.0	36.0
8	34.81181547854004	36.0	36.0	36.0	32.0	36.0
9	34.78998447409279	36.0	36.0	36.0	32.0	36.0
10	34.6818583721481	36.0	36.0	36.0	32.0	36.0
11	34.86190363411489	36.0	36.0	36.0	32.0	36.0
12	34.80042367306123	36.0	36.0	36.0	32.0	36.0
13	34.762427304544616	36.0	36.0	36.0	32.0	36.0
14	34.68318202152575	36.0	36.0	36.0	32.0	36.0
15	34.69471856003789	36.0	36.0	36.0	32.0	36.0
16	34.63760164206205	36.0	36.0	36.0	32.0	36.0
17	34.57460066840346	36.0	36.0	36.0	32.0	36.0
18	34.49697113233862	36.0	36.0	36.0	32.0	36.0
19	34.46347464540407	36.0	36.0	36.0	32.0	36.0
20	34.33519907370859	36.0	36.0	36.0	32.0	36.0
21	34.273795426436145	36.0	36.0	36.0	32.0	36.0
22	34.30806031420226	36.0	36.0	36.0	32.0	36.0
23	34.285010920765245	36.0	36.0	36.0	32.0	36.0
24	34.318328465040395	36.0	36.0	36.0	32.0	36.0
25	34.26568774505934	36.0	36.0	36.0	32.0	36.0
26	34.177076919028444	36.0	36.0	36.0	32.0	36.0
27	34.18085050393411	36.0	36.0	36.0	32.0	36.0
28	34.09205020920502	36.0	36.0	36.0	32.0	36.0
29	33.9803084129365	36.0	36.0	36.0	32.0	36.0
30	33.87865056182732	36.0	36.0	36.0	32.0	36.0
31	33.76656930080787	36.0	36.0	36.0	32.0	36.0
32	33.59405805110392	36.0	36.0	36.0	27.0	36.0
33	33.3028209783953	36.0	36.0	36.0	21.0	36.0
34	33.062482566248256	36.0	36.0	36.0	14.0	36.0
35	32.811736533249125	36.0	36.0	36.0	14.0	36.0
36	32.683021499434226	36.0	36.0	36.0	14.0	36.0
37	32.53406226151943	36.0	36.0	36.0	14.0	36.0
38	32.13796742190995	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	11.0
20	29.0
21	48.0
22	106.0
23	216.0
24	532.0
25	1220.0
26	2407.0
27	4748.0
28	8017.0
29	12475.0
30	18062.0
31	24445.0
32	32003.0
33	45693.0
34	82197.0
35	147797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.97381926131788	17.595940774895126	16.023991115181566	14.40624884860543
2	12.342569932370202	26.23378332149154	40.27762427304545	21.146022473092813
3	18.143469908686612	38.40241046288256	28.376884818820557	15.077234809610275
4	9.490539722638879	18.795821162600983	32.66308781347859	39.050551301281544
5	8.747664535143812	43.580958395831686	28.58556353780164	19.08581353122286
6	24.06870381676175	36.64922843729605	20.762194480116207	18.519873265825982
7	21.469491512043135	38.01752063766911	22.499993421208444	18.012994429079313
8	32.496065851591815	25.35091551186034	21.13887675457756	21.014141881970286
9	31.231808923298786	13.122312457565119	21.562445722827203	34.08343289630889
10	18.846231779940894	29.726820821721706	28.185007118289928	23.241940280047473
11	29.49964604424842	26.781423770562984	21.88393935645844	21.834990828730156
12	26.70015710154233	30.959529905870653	29.05431187156094	13.28600112102608
13	33.03500706562213	23.976274246136274	28.99168177595794	13.997036912283656
14	27.278229520275783	20.694455409068183	34.29436067471909	17.732954395936947
15	23.254388042419937	24.713560169469226	38.3568853451225	13.675166442988344
16	16.524565143022553	28.507934001736796	34.15357490592353	20.81392594931712
17	15.17775848004	26.307202442041	34.867240335780636	23.647798742138367
18	17.31533380700508	21.517854793294912	43.19412647035604	17.972684929343966
19	17.94400147364543	25.50196047472435	43.611747059287914	12.942290992342306
20	22.163890423936213	17.497171127075603	45.82800452619668	14.510933922791505
21	24.192784400410517	20.69998157943212	39.068445567221914	16.03878845293545
22	21.139291804383063	21.97822151112608	39.84731900381044	17.03516768068041
23	17.032709665535116	23.926475619062657	41.39470013947001	17.646114575932213
24	15.99931580747875	27.234809610273413	41.606010368148205	15.159864214099628
25	18.837448586744	23.351552200079475	40.84745361294075	16.963545600235783
26	15.88751845351031	24.15416476978177	43.064769518616664	16.893547258091253
27	17.69431776615817	26.193327000149996	42.26663052717172	13.84572470652011
28	15.844583037288492	24.304097260598407	43.46543512012842	16.385884581984683
29	14.176205889318702	26.617720586300358	44.85618799505276	14.349885529328176
30	16.429830793926474	25.84984605668272	43.39412120733665	14.326201942054157
31	14.088839767374543	27.200863135180654	41.63364121996789	17.07665587747691
32	13.51753901213126	28.424515144338308	41.512065471961265	16.54588037156917
33	14.20199468435041	27.628483460961554	41.18075840109471	16.988763453593325
34	14.18383726744033	28.443198863187813	41.71732322833609	15.655640641035761
35	16.490092365990368	28.286360885239866	40.55024867766638	14.67329807110339
36	15.729849214494354	30.506828767664008	39.56895871161285	14.194363306228782
37	16.26615089076603	31.58680034735928	39.445540906818245	12.701507855056446
38	15.138325671234101	31.65951332731593	38.176727393298584	15.025433608151387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2701.0
1	1937.5
2	1174.0
3	1174.0
4	3859.5
5	6545.0
6	6545.0
7	7511.0
8	8477.0
9	8077.0
10	7677.0
11	7677.0
12	9166.0
13	10655.0
14	11856.5
15	13058.0
16	13058.0
17	18993.0
18	24928.0
19	24928.0
20	25990.5
21	27053.0
22	20842.5
23	14632.0
24	14632.0
25	9876.0
26	5120.0
27	5120.0
28	7224.0
29	9328.0
30	11526.5
31	13725.0
32	13725.0
33	11215.0
34	8705.0
35	8705.0
36	8465.5
37	8226.0
38	9822.5
39	11419.0
40	11419.0
41	14911.5
42	18404.0
43	23156.5
44	27909.0
45	27909.0
46	40313.5
47	52718.0
48	52718.0
49	45904.5
50	39091.0
51	28903.5
52	18716.0
53	18716.0
54	15342.0
55	11968.0
56	11968.0
57	11390.0
58	10812.0
59	9641.5
60	8471.0
61	8471.0
62	7677.0
63	6883.0
64	5673.0
65	4463.0
66	4463.0
67	3686.5
68	2910.0
69	2910.0
70	2333.0
71	1756.0
72	1411.5
73	1067.0
74	1067.0
75	769.0
76	471.0
77	471.0
78	452.5
79	434.0
80	328.0
81	222.0
82	222.0
83	205.0
84	188.0
85	188.0
86	114.5
87	41.0
88	38.0
89	35.0
90	35.0
91	22.0
92	9.0
93	9.0
94	9.0
95	9.0
96	8.0
97	7.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009473434909607642
2	0.0
3	0.0
4	0.0
5	0.0
6	5.263019394226468E-4
7	2.631509697113234E-4
8	0.0010526038788452937
9	0.004210415515381175
10	7.894529091339701E-4
11	0.004999868424515145
12	2.631509697113234E-4
13	2.631509697113234E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	5.263019394226468E-4
23	0.0
24	0.0
25	2.631509697113234E-4
26	2.631509697113234E-4
27	2.631509697113234E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.631509697113234E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	380010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.236545856080692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.64016598545373	19.512306660000426
2	15.299684175898904	8.334210992808453
3	5.221100092528593	4.2661419626802655
4	2.3322455873828773	2.5408925555358235
5	1.2887996755740532	1.7551225731537308
6	0.7873928366087435	1.2867516660626097
7	0.5242601934915114	0.9995325760384501
8	0.4056533675904605	0.8838877238440908
9	0.25116617448743006	0.6156809126030928
>10	1.8199580282973342	9.429853315859567
>50	0.1973448463385158	3.8075574336120375
>100	0.17342425890354418	9.745566224630839
>500	0.027907352007466885	5.42494137696442
>1k	0.024917278578095434	13.2805735610415
>5k	0.002990073429371452	4.848351330693782
>10k+	0.002990073429371452	13.268629134470922
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	22269	5.8601089445014605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	16246	4.27515065393016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	10363	2.7270334991184444	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7174	1.8878450567090341	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	5478	1.4415410120786296	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	5208	1.3704902502565721	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	4915	1.2933870161311545	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3562	0.9373437541117339	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3505	0.9223441488381884	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3079	0.8102418357411647	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	2864	0.7536643772532302	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	2595	0.6828767664008842	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	2518	0.6626141417331123	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	2479	0.6523512539143707	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	2243	0.5902476250624984	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	2191	0.5765637746375095	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1779	0.46814557511644433	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1604	0.42209415541696277	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1477	0.38867398226362465	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1418	0.37314807505065656	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1372	0.3610431304439357	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	1333	0.3507802426251941	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	1230	0.32367569274492775	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	1157	0.30446567195600116	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	1131	0.2976237467435067	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	1130	0.2973605957737954	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1117	0.29393963316754823	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	1114	0.2931501802584142	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.283150443409384	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	1032	0.27157180074208576	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	1001	0.2634141206810347	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	921	0.24236204310412882	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	913	0.24025683534643827	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	887	0.23341491013394383	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	878	0.23104655140654193	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	868	0.2284150417094287	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	843	0.22183626746664561	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	807	0.212362832557038	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	802	0.21104707770848138	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	798	0.20999447382963604	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	774	0.20367885055656432	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	762	0.20052103892002843	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	708	0.18631088655561695	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	707	0.18604773558590562	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	704	0.18525828267677166	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	704	0.18525828267677166	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	686	0.18052156522196786	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	685	0.18025841425225653	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	657	0.17289018710033946	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	653	0.17183758322149417	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	637	0.167627167706113	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	631	0.16604826188784508	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	614	0.16157469540275254	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	608	0.15999578958448463	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	604	0.1589431857056393	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	550	0.14473303334122786	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	543	0.14289097655324862	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	522	0.13736480618931082	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	518	0.1363122023104655	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	500	0.13157548485566167	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	500	0.13157548485566167	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	492	0.1294702770979711	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA	483	0.1271019183705692	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	482	0.12683876740085787	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	475	0.12499671061287862	No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT	471	0.12394410673403332	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	466	0.1226283518854767	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	453	0.1192073892792295	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT	441	0.11604957764269361	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	441	0.11604957764269361	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	390	0.10262887818741612	No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAA	386	0.10157627430857083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.6315096971132336E-4	0.0
6	0.0	0.0	0.0	2.6315096971132336E-4	0.0
7	0.0	0.0	0.0	2.6315096971132336E-4	0.0
8	0.0	0.0	0.0	2.6315096971132336E-4	0.0
9	0.0	0.0	0.0	2.6315096971132336E-4	0.0
10	0.0	0.0	0.0	2.6315096971132336E-4	0.0
11	0.0	0.0	0.0	2.6315096971132336E-4	0.0
12	0.0	0.0	0.0	2.6315096971132336E-4	0.0
13	0.0	0.0	0.0	2.6315096971132336E-4	0.0
14	0.0	0.0	0.0	2.6315096971132336E-4	0.0
15	0.0	0.0	0.0	2.6315096971132336E-4	0.0
16	0.0	0.0	0.0	5.263019394226467E-4	0.0
17	0.0	0.0	0.0	5.263019394226467E-4	0.0
18	0.0	0.0	0.0	0.0013157548485566168	0.0
19	0.0	0.0	0.0	0.0013157548485566168	0.0
20	0.0	0.0	0.0	0.0018420567879792638	0.0
21	0.0	0.0	0.0	0.0028946606668245572	0.0
22	0.0	0.0	0.0	0.006841925212494408	0.0
23	0.0	0.0	0.0	0.01947317175863793	0.0
24	0.0	0.0	0.0	0.037630588668719246	0.0
25	0.0	0.0	0.0	0.04420936291150233	0.0
26	0.0	0.0	0.0	0.06131417594273835	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCTC	20	0.0037511352	31.998684	19
CGACCAT	30	8.408466E-4	26.665571	10
CCCTCCA	35	0.002064505	22.856203	31
AACATGG	50	2.7892975E-5	22.402027	2
GAACATG	40	0.004462088	20.012344	1
TAGTTCC	40	0.0044757086	20.00181	4
ATACATG	90	2.0132575E-8	19.567625	1
CGTTAAA	45	0.008808309	17.78875	1
CTACATG	45	0.008808309	17.78875	1
TTACGCG	45	0.0088350745	17.779387	5
GGACAGC	45	0.0088350745	17.779387	6
CCAATTA	45	0.008841775	17.777046	28
GTCACTA	45	0.008841775	17.777046	21
CAAGACA	55	0.0013615426	17.456125	4
AAAGGAT	55	0.0013615426	17.456125	5
GTTCAAA	60	0.0024278483	16.009874	1
TACCCAC	60	0.0024385394	15.999343	30
GCAAATT	60	0.0024385394	15.999343	24
CGCGCAA	60	0.0024385394	15.999343	21
CGCAAAT	60	0.0024385394	15.999343	23
>>END_MODULE