FastQCFastQC Report
Thu 2 Feb 2017
SRR4062962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781458
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33470.4283019688838044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24290.31082924482185864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13320.17045061922713697No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12460.15944554921697646No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10460.1338523631468358No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.13359643128613438No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9280.11875238336545278No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9270.11862441743510208No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8850.11324984836037254No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8800.11261001870861902No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8510.10889900672844861No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8400.10749138149459088No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8030.10275664207161485No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG7830.1001973234646008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC604.7175326E-621.3332483
CTAGACA951.724402E-920.2078594
GTTATTC1301.2732926E-1118.4614643
TAGCGGC1950.018.05005530
TCTAGCG1950.018.05005528
ATCGTTT1950.018.05005529
ATACCGT1700.017.8799936
CTAGCGG2000.017.59880329
TTATACC550.001365322617.4522424
ACCGTCG1601.8189894E-1216.9988448
CGCAAGA2450.016.9882222
GCATCGT2200.016.72613527
ACGAACG1151.8382707E-816.69558515
CGTCGTA1651.8189894E-1216.48478110
TACCGTC1651.8189894E-1216.4837287
CATCGTT2150.016.3709828
GTCTAAC600.002423359316.0173631
AACGAAC1304.793037E-915.99993514
TCGTTTA2200.015.99891330
TATACAG1501.1459633E-1015.9978895
Produced by FastQC (version 0.11.5)