##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062962_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781458
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16506709253728	32.0	32.0	32.0	32.0	32.0
2	30.815640763803046	32.0	32.0	32.0	32.0	32.0
3	30.878501467769222	32.0	32.0	32.0	32.0	32.0
4	30.92534083725549	32.0	32.0	32.0	32.0	32.0
5	30.874007304295304	32.0	32.0	32.0	32.0	32.0
6	34.50367390186037	36.0	36.0	36.0	32.0	36.0
7	34.41195943991872	36.0	36.0	36.0	32.0	36.0
8	34.40609220201214	36.0	36.0	36.0	32.0	36.0
9	34.50769075241408	36.0	36.0	36.0	32.0	36.0
10	34.26995564700854	36.0	36.0	36.0	32.0	36.0
11	34.528693800562536	36.0	36.0	36.0	32.0	36.0
12	34.34922030358637	36.0	36.0	36.0	32.0	36.0
13	34.43376483445048	36.0	36.0	36.0	32.0	36.0
14	34.33765602246058	36.0	36.0	36.0	32.0	36.0
15	34.31304817405414	36.0	36.0	36.0	32.0	36.0
16	34.29281420114709	36.0	36.0	36.0	32.0	36.0
17	34.233119374297786	36.0	36.0	36.0	32.0	36.0
18	34.25804841718941	36.0	36.0	36.0	32.0	36.0
19	34.24048509324877	36.0	36.0	36.0	32.0	36.0
20	34.21107852245418	36.0	36.0	36.0	32.0	36.0
21	34.19151381136286	36.0	36.0	36.0	32.0	36.0
22	34.15734050966271	36.0	36.0	36.0	32.0	36.0
23	34.12949384355909	36.0	36.0	36.0	32.0	36.0
24	34.11021705581106	36.0	36.0	36.0	32.0	36.0
25	34.08890944874837	36.0	36.0	36.0	32.0	36.0
26	34.04551364244784	36.0	36.0	36.0	32.0	36.0
27	34.045348566397685	36.0	36.0	36.0	32.0	36.0
28	34.03444842845041	36.0	36.0	36.0	32.0	36.0
29	33.9963516913257	36.0	36.0	36.0	32.0	36.0
30	33.96827084756954	36.0	36.0	36.0	32.0	36.0
31	33.97772113152594	36.0	36.0	36.0	32.0	36.0
32	33.92779778311822	36.0	36.0	36.0	32.0	36.0
33	33.89494253050068	36.0	36.0	36.0	32.0	36.0
34	33.88033649921045	36.0	36.0	36.0	32.0	36.0
35	33.83815380992964	36.0	36.0	36.0	32.0	36.0
36	33.794665868159264	36.0	36.0	36.0	32.0	36.0
37	33.77832333919417	36.0	36.0	36.0	32.0	36.0
38	33.17457112218443	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	2.0
9	2.0
10	6.0
11	7.0
12	5.0
13	1.0
14	179.0
15	354.0
16	400.0
17	457.0
18	604.0
19	761.0
20	934.0
21	1216.0
22	1844.0
23	2582.0
24	3736.0
25	5528.0
26	7648.0
27	11062.0
28	15312.0
29	21020.0
30	27449.0
31	36993.0
32	50030.0
33	74306.0
34	165076.0
35	353943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77753519574832	17.31561630356237	11.52888178226042	25.37796671842889
2	16.313729406658243	20.230289481534975	37.63554442476924	25.82043668703754
3	18.994560696230884	24.690855570486978	28.45677353298778	27.857810200294363
4	12.445852683467425	15.60901574415209	35.78272225513896	36.162409317241526
5	14.345766945590096	36.88558679492282	33.421928525817805	15.346717733669287
6	33.66489433172736	35.52219897498896	17.225560013052572	13.587346680231107
7	29.6102024807507	30.911617657785396	21.19361653936173	18.284563322102176
8	28.449147358641714	32.49442705520734	19.784529863562025	19.271895722588916
9	27.962674801908232	14.151148625130524	18.65057021764501	39.235606355316236
10	16.08642351309226	26.92020259669795	31.541075994236383	25.452297895973402
11	37.13181895544525	21.425491046691825	22.132388414766016	19.310301583096912
12	24.694313951568837	24.2666414141091	28.77332715669425	22.26571747762781
13	29.8959110566186	19.522212168410867	25.241024840308647	25.340851934661885
14	23.89525724326545	19.913716867955582	25.49870037792063	30.692325510858335
15	25.104410787813475	26.927167795783635	23.20165777324396	24.766763643158935
16	25.261496838233505	25.93864342876011	24.262478451227143	24.53738128177924
17	23.725190800613838	25.934676627676744	25.688936444165144	24.651196127544274
18	24.73227951451987	24.780909430620465	26.918834110558837	23.56797694430083
19	25.32022856557823	25.217719591243863	26.101907485970603	23.360144357207304
20	25.571594937356828	24.11960430503833	25.876684455023614	24.432116302581232
21	27.016349889686875	24.345543708056695	24.725752867885316	23.912353534371114
22	25.548979082550023	24.589937356428486	25.39412980464676	24.466953756374735
23	23.98942392108382	24.372973152833556	25.97269467331127	25.664908252771358
24	24.38427238939684	25.478273556604446	25.77557874043965	24.36187531355906
25	24.621029836381137	24.66480146622161	25.688955214711363	25.02521348268589
26	24.07022968763199	25.67136212726635	26.246541118770494	24.011867066331167
27	25.00319943485183	25.04248849483227	25.386875662283014	24.567436408032886
28	24.174986018881228	24.995552916454976	26.241120229943014	24.588340834720782
29	23.98452487448537	25.297965274391686	26.376437675250163	24.34107217587278
30	24.034280390766767	25.44616463494225	26.43600643941042	24.083548534880567
31	24.425530063372133	25.37751978459617	25.18735219140637	25.009597960625324
32	23.972710554504026	25.651746030527217	25.213814673899826	25.161728741068927
33	23.957020236987628	24.963046669337118	25.80922995017859	25.270703143496664
34	24.530977438027847	25.420453338350235	25.832299877521088	24.216269346100834
35	25.32335723263035	25.023357488602826	25.910046151837758	23.743239126929073
36	24.071983658926158	25.897683990662244	25.481094114756115	24.549238235655483
37	24.921485265849483	25.743106444735098	25.4600182753122	23.875390014103218
38	24.182633489119695	25.639739343649705	25.630141333893004	24.5474858333376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	917.0
1	649.0
2	381.0
3	381.0
4	775.5
5	1170.0
6	1170.0
7	1310.5
8	1451.0
9	1391.0
10	1331.0
11	1331.0
12	1661.5
13	1992.0
14	2383.0
15	2774.0
16	2774.0
17	4120.0
18	5466.0
19	5466.0
20	6241.5
21	7017.0
22	6948.0
23	6879.0
24	6879.0
25	7640.0
26	8401.0
27	8401.0
28	10797.0
29	13193.0
30	15972.0
31	18751.0
32	18751.0
33	22769.5
34	26788.0
35	26788.0
36	29660.0
37	32532.0
38	37006.5
39	41481.0
40	41481.0
41	44664.0
42	47847.0
43	54067.0
44	60287.0
45	60287.0
46	64065.0
47	67843.0
48	67843.0
49	70705.5
50	73568.0
51	72586.5
52	71605.0
53	71605.0
54	68127.0
55	64649.0
56	64649.0
57	62787.0
58	60925.0
59	54608.5
60	48292.0
61	48292.0
62	45297.5
63	42303.0
64	34998.0
65	27693.0
66	27693.0
67	22923.5
68	18154.0
69	18154.0
70	14477.5
71	10801.0
72	8384.5
73	5968.0
74	5968.0
75	4520.0
76	3072.0
77	3072.0
78	2991.5
79	2911.0
80	2235.0
81	1559.0
82	1559.0
83	1650.5
84	1742.0
85	1742.0
86	1136.0
87	530.0
88	464.0
89	398.0
90	398.0
91	295.5
92	193.0
93	156.0
94	119.0
95	119.0
96	122.5
97	126.0
98	126.0
99	237.5
100	349.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1236150887187795
2	0.056432975284660215
3	0.01382032047787597
4	0.0031991482587675852
5	1.2796593035070342E-4
6	3.838977910521103E-4
7	1.2796593035070342E-4
8	5.118637214028137E-4
9	2.5593186070140683E-4
10	5.118637214028137E-4
11	0.0019194889552605517
12	0.002431352676663365
13	0.013692354547525267
14	0.009853376637004165
15	0.02162624222926888
16	0.011644899661914012
17	0.018555059900851996
18	0.0058864327961323576
19	0.008061853612094317
20	0.0061423646568337644
21	0.0058864327961323576
22	0.0067821943085872825
23	0.009213546985250646
24	0.013564388617174565
25	0.016635570945591444
26	0.017403366527695665
27	0.008957615124549241
28	0.006014398726483061
29	0.009597444776302757
30	0.002559318607014069
31	0.005374569074729545
32	0.007294058029990095
33	0.008573717333497129
34	0.01266862710471964
35	0.015867775363487227
36	0.01458811605998019
37	0.00972541070665346
38	0.0058864327961323576
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	781458.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.75042354150197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.88140024578743	51.79711992431621
2	10.680231929056433	13.19017690281415
3	2.625535542951392	4.863837954015474
4	0.9705024544419464	2.3971575043942965
5	0.47684152620367487	1.4722583102626567
6	0.27276209426037806	1.010590490798726
7	0.1761861693125786	0.7615699404044556
8	0.13046769507742664	0.6445148343611701
9	0.09301988662320113	0.5169615657088648
>10	0.5322296996023737	6.38230959834023
>50	0.07480181320101072	3.2502576235349876
>100	0.07833308138584472	9.663552583827506
>500	0.0064411817563355045	2.7096746681540624
>1k	0.001246680339935904	1.3400180990672392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3347	0.4283019688838044	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2429	0.31082924482185864	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1332	0.17045061922713697	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1246	0.15944554921697646	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1046	0.1338523631468358	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1044	0.13359643128613438	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	928	0.11875238336545278	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	927	0.11862441743510208	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	885	0.11324984836037254	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	880	0.11261001870861902	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	851	0.10889900672844861	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	840	0.10749138149459088	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	803	0.10275664207161485	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	783	0.1001973234646008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.2796593035070342E-4
9	0.0	0.0	0.0	0.0	1.2796593035070342E-4
10	0.0	0.0	0.0	0.0	1.2796593035070342E-4
11	0.0	0.0	0.0	0.0	1.2796593035070342E-4
12	0.0	0.0	0.0	0.0	5.118637214028137E-4
13	0.0	0.0	0.0	0.0	5.118637214028137E-4
14	0.0	0.0	0.0	0.0	5.118637214028137E-4
15	0.0	0.0	0.0	0.0	7.677955821042206E-4
16	0.0	0.0	0.0	0.0	7.677955821042206E-4
17	0.0	0.0	0.0	0.0	7.677955821042206E-4
18	0.0	0.0	0.0	0.0	8.95761512454924E-4
19	0.0	0.0	0.0	1.2796593035070342E-4	8.95761512454924E-4
20	0.0	0.0	0.0	2.5593186070140683E-4	0.0011516933731563308
21	0.0	0.0	0.0	5.118637214028137E-4	0.0011516933731563308
22	0.0	0.0	0.0	0.0010237274428056273	0.0011516933731563308
23	0.0	0.0	0.0	0.0023033867463126616	0.0011516933731563308
24	0.0	0.0	0.0	0.003838977910521103	0.0011516933731563308
25	0.0	0.0	0.0	0.00524660314437884	0.0011516933731563308
26	0.0	0.0	0.0	0.007422023960340799	0.0011516933731563308
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAC	60	4.7175326E-6	21.333248	3
CTAGACA	95	1.724402E-9	20.207859	4
GTTATTC	130	1.2732926E-11	18.461464	3
TAGCGGC	195	0.0	18.050055	30
TCTAGCG	195	0.0	18.050055	28
ATCGTTT	195	0.0	18.050055	29
ATACCGT	170	0.0	17.879993	6
CTAGCGG	200	0.0	17.598803	29
TTATACC	55	0.0013653226	17.452242	4
ACCGTCG	160	1.8189894E-12	16.998844	8
CGCAAGA	245	0.0	16.988222	2
GCATCGT	220	0.0	16.726135	27
ACGAACG	115	1.8382707E-8	16.695585	15
CGTCGTA	165	1.8189894E-12	16.484781	10
TACCGTC	165	1.8189894E-12	16.483728	7
CATCGTT	215	0.0	16.37098	28
GTCTAAC	60	0.0024233593	16.017363	1
AACGAAC	130	4.793037E-9	15.999935	14
TCGTTTA	220	0.0	15.998913	30
TATACAG	150	1.1459633E-10	15.997889	5
>>END_MODULE