FastQCFastQC Report
Thu 2 Feb 2017
SRR4062962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781458
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41490.5309306450250686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31710.4057799651420806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18220.23315392509898164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.17723281353572423No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12130.15522267351540325No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11650.1490803088585695No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9660.1236150887187795No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8650.11069052975335847No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8510.10889900672844861No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8310.10633968812143454No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8300.10621172219108385No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8280.10595579033038244No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8140.10416426730547258No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8050.10301257393231626No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8030.10275664207161485No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8020.10262867614126414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG200.003753558531.99865727
TACCGGA350.002065687722.8576452
GTTTACG453.5363718E-421.33243830
TATACTG951.718945E-920.2122635
AGACACG400.004479987520.001725
CGACAAT507.195152E-419.19919419
ATATCGA507.195152E-419.19919415
TATCGAC601.1379567E-418.66588216
CCGAGTT450.00884994917.7770331
TATCCGA450.00884994917.7770328
TCGACAA550.001364499717.45381418
TCGTTTA1950.017.23004530
CGGCATC1950.017.23004525
TCTAGCG1551.0913936E-1116.51543428
ATCGTTT2050.016.38955729
TACCGTC1703.6379788E-1216.0013777
ACGATCA1203.1021045E-816.0013759
CGATCAG1407.403287E-1016.00035310
GATTACT600.00244060916.000352
CATCGTT2100.015.99932928
Produced by FastQC (version 0.11.5)